14 research outputs found
Structural and ultrastructural study of the rabbit kidney exposed to carbamate insecticide
The effects of simulated microgravity on skeletal muscle of Japanese quail: transmission electron microscopic study
Potential influence of prenatal 2.45 GHz radiofrequency electromagnetic field exposure on Wistar albino rat testis
An ever-increasing use of wireless devices
over the last decades has forced scientists to clarify their
impact on living systems. Since prenatal development is
highly sensitive to numerous noxious agents, including
radiation, we focused on the assessment of potential
adverse effects of microwave radiation (MR) on
testicular development. Pregnant Wistar albino rats (3
months old, weighing 282±8 g) were exposed to pulsed
MR at a frequency of 2.45 GHz, mean power density of
2.8 mW/cm2, and a specific absorption rate of 1.82 W/kg
for 2 hours/day throughout pregnancy. Male offspring
were no longer exposed to MR following birth. Samples
of biological material were collected after reaching
adulthood (75 days). In utero MR exposure caused
degenerative changes in the testicular parenchyma of
adult rats. The shape of the seminiferous tubules was
irregular, germ cells were degenerated and often
desquamated. The diameters of the seminiferous tubules
and the height of the germinal epithelium were
significantly decreased (both at **p<0.01), while the
interstitial space was significantly increased (**p<0.01)
when compared to the controls. In the group of rats
prenatally exposed to MR, the somatic and germ cells
were rich in vacuoles and their organelles were often
altered. Necrotizing cells were more frequent and empty
spaces between Sertoli cells and germ cells were
observed. The Leydig cells contained more lipid
droplets. An increased Fluoro Jade - C and superoxide
dismutase 2 positivity was detected in the rats exposed
to MR. Our results confirmed adverse effects of MR on
testicular development
The potential adverse effect of 2.45 GHz microwave radiation on the testes of prenatally exposed peripubertal male rats
In utero development of organs is easily
influenced by many environmental factors. The aim of
this study was to elucidate the effect of microwave
radiation (MR) at a frequency of 2.45 GHz and a
specific absorption rate of 1.73 W/kg on intrauterine
development of testis. Pregnant albino rats were exposed
to whole-body MR for 2 hours per day throughout the
pregnancy. Male offspring (n=12, age 35 days) were not
exposed to MR after birth. The study revealed that MR
applied in utero induced apparent structural changes in
the testes, such as irregular shape of seminiferous
tubules, significant decrease in the diameter of
seminiferous tubules (p<0.05) and in the height of the
germinal epithelium (p<0.01), disorganisation of germ
cells, desquamations of immature germ cells, formation
of giant multinucleated cells, and significant (p<0.01)
expansion of the interstitium. At the level of
transmission electron microscopy, there were observed
basement membrane irregularities in seminiferous
tubules, vacuolation of the cytoplasm and adversely
affected organelles in Sertoli cells, germ cells, Leydig
cells, peritubular and endothelial cells. The tight
junctions between adjacent Sertoli cells were often
incomplete, and necrotizing germ cells were more
numerous in experimental animals compared to controls.
Enhanced necrotizations of germ cells proved by a
Fluoro Jade C method, and declined germ cells
proliferation confirmed by proliferating cell nuclear
antigen analysis, were detected in MR exposed animals.
Our results revealed that the prenatal exposure to MR
had an adverse effect on the postnatal testicular
development in rats