Analysis of Varroa destructor infestation of southern African honeybee populations

The discovery of the honeybee-specific ectoparasitic mite Varroa destructor in South Africa in October 1997 raised the spectre of massive honeybee colony losses as has occurred in most parts of the world where the varroa mite has been found. This was particularly concerning in Africa because of the importance of honeybees in the pollination of indigenous and commercial crops, and because of the numbers of small-scale beekeepers in Africa. The mite has now spread throughout South Africa and is found in almost all honeybee populations, both commercial and wild, and is also now present in most neighbouring countries. Varroa has not left a trail of destruction in South Africa as had been expected and no large scale collapse of the honeybee population occurred, despite the majority of beekeepers deciding not to protect their hives with chemical varroacides. Some colony losses did occur at the front of the varroa spread, and all colonies were found to be deleteriously affected by the mite which developed populations of 50 000 and more in some colonies. Infected colonies were also not as efficient as pollinators as uninfected colonies. Colonies exhibited all the same varroa effects witnessed in other parts of the world, with the exception that the majority of colonies did not die as a result of the infestation. The relative tolerance of African bees to the varroa mite has been confirmed by the long-term monitoring of both wild honeybee populations and commercial stock, and by population dynamic studies of the mites. In both wild and managed honeybee populations varroa appears to have been reduced to the status of an incidental pest. The development of mite tolerance took 3-5 years in the Cape honeybee (Apis mellifera capensis) and 6-7 years in the Savanna honeybee (Apis mellifera scutellata). The rapid development of mite tolerance in the Cape bee is thought to be due to the well developed removal of varroa-infested brood and the short post-capping period of worker brood. Together these resulted in a very rapid increase in infertile mites in the colony, the collapse of the mite population, and varroa tolerance. Tolerance does not develop as rapidly in Savanna honeybees as the post-capping period in these bees is similar to that of European bees and does not result in as many infertile mites. Nonetheless, varroa tolerance in Savanna bees develops more rapidly than would be the case in European bees because of more effective hygienic removal of varroa-infested brood. In both Cape and Savanna bees, the absence of varroacide applications and a “live-and-let-die” approach to the wild and commercial honeybee populations was crucial to the developed of population-wide varroa tolerance, in contrast to the selective breeding and pesticide treadmill practised in most parts of the world in an effort to get rid of the varroa mite. Varroa destructor is concluded not to be a serious threat to honeybees and beekeeping in Africa, and efforts should be made to prevent the use of pesticides and techniques that could hinder the development of natural mite tolerance in Africa.Dissertation (MSc (Entomology))--University of Pretoria, 2007.Zoology and Entomologyunrestricte

A worldwide survey of genome sequence variation provides insight into the evolutionary history of the honeybee Apis mellifera

The honeybee Apis mellifera has major ecological and economic importance. We analyze patterns of genetic variation at 8.3 million SNPs, identified by sequencing 140 honeybee genomes from a worldwide sample of 14 populations at a combined total depth of 634×. These data provide insight into the evolutionary history and genetic basis of local adaptation in this species. We find evidence that population sizes have fluctuated greatly, mirroring historical fluctuations in climate, although contemporary populations have high genetic diversity, indicating the absence of domestication bottlenecks. Levels of genetic variation are strongly shaped by natural selection and are highly correlated with patterns of gene expression and DNA methylation. We identify genomic signatures of local adaptation, which are enriched in genes expressed in workers and in immune system– and sperm motility–related genes that might underlie geographic variation in reproduction, dispersal and disease resistance. This study provides a framework for future investigations into responses to pathogens and climate change in honeybees.Swedish Research Council Formas (grant 2010-1295).http://www.nature.comhb201

Estimating the density of honeybee colonies across their natural range to fill the gap in pollinator decline censuses

Although pollinator declines are a global biodiversity threat, the demography of the western honeybee (Apis mellifera) has not been considered by conservationists because it is biased by the activity of beekeepers. To fill this gap in pollinator decline censuses and to provide a broad picture of the current status of honeybees across their natural range, we used microsatellite genetic markers to estimate colony densities and genetic diversity at different locations in Europe, Africa, and central Asia that had different patterns of land use. Genetic diversity and colony densities were highest in South Africa and lowest in Northern Europe and were correlated with mean annual temperature. Confounding factors not related to climate, however, are also likely to influence genetic diversity and colony densities in honeybee populations. Land use showed a significantly negative influence over genetic diversity and the density of honeybee colonies over all sampling locations. In Europe honeybees sampled in nature reserves had genetic diversity and colony densities similar to those sampled in agricultural landscapes, which suggests that the former are not wild but may have come from managed hives. Other results also support this idea: putative wild bees were rare in our European samples, and the mean estimated density of honeybee colonies on the continent closely resembled the reported mean number of managed hives. Current densities of European honeybee populations are in the same range as those found in the adverse climatic conditions of the Kalahari and Saharan deserts, which suggests that beekeeping activities do not compensate for the loss of wild colonies. Our findings highlight the importance of reconsidering the conservation status of honeybees in Europe and of regarding beekeeping not only as a profitable business for producing honey, but also as an essential component of biodiversity conservation.This project was funded by the BEESHOP European network (FOOD-CT-2006-022568) and the National Research Foundation of South Africa