ELK1 Gene Transfection Effect in Prostate Cancer Cell Line Proliferation Activity

Three type of prostate cancer cell line was selected for this study (PC3, DU145 & LNCaP) as a model, transfected by liposome with the ELK1 gene ( control & knock down) , then detecting the proliferation ability of the cultured cell lines by the Mtt or (proliferation) assay that shows clear effect of ELK1 gene in this cells comparing with the control cell transfected with the knock down gene, using for each type of cells 6 repeats ,for each type there was two groups 1st for control ELK1 and the 2nd was knock down or (sh) ELK1, all works was done in Johns Hopkins University / School of Medicine / Pathology Department ( MD, USA

The Elk1 gene effect on prostate cancer cell line wound healing ability

In this study prostate cancer cell line model was used selecting three type of them (Pc3, DU145 & LNCaP) , gene transfecting was done by using the liposome into the cell culture cells then detecting the healing ability by the Wound Healing or (scratching) test that shows clear effect of ELK1 gene in this cells comparing with the control cell transfected with the knock down gene, using for each type of cells 6 repeats ,for each type there was two groups 1st for control ELK1 and the 2nd was knock down or (sh) ELK1, all works was done in Johns Hopkins University / School of Medicine / Pathology Department ( MD, USA