ESTIMATION OF THE EFFECTS OF CHITOSAN-IRON NANOCOMPOSITES DEVELOPED BY DIFFERENT PROCESSES ON RH. GRACILIS CNMN-Y-30 YEAST

Abstract. The paper provides information on the estimation of the effects of iron chitosan nanocomposites, elaborated by different procedures on pigmented yeast Rh. gracilis CNMN-Y-30. It was found that the initial amount of chitosan, the concentration of Fe3O4 nanoparticles and the volume of nanocomposite used for growing yeasts are the main factors that influence the efficiency of chitosan-iron nanocomposites. Microbiological indices adequately reflect the effects of chitosan-iron nanocomposites in the process of evaluating the action of nanocomposites obtained by different processes on the representative yeast Rh. gracilis CNMN-Y-30 and it is recommended to test the degree of influence of the nanocomposite. This information can be used by specialists in the food industry, microbiology, medicine, cosmetology, environmental protection, etc., where nanocomposites have applications

ESTIMATION OF THE EFFECTS OF CHITOSAN-IRON NANOCOMPOSITES DEVELOPED BY DIFFERENT PROCESSES ON RH. GRACILIS CNMN-Y-30 YEAST

Abstract. The paper provides information on the estimation of the effects of iron chitosan nanocomposites, elaborated by different procedures on pigmented yeast Rh. gracilis CNMN-Y-30. It was found that the initial amount of chitosan, the concentration of Fe3O4 nanoparticles and the volume of nanocomposite used for growing yeasts are the main factors that influence the efficiency of chitosan-iron nanocomposites. Microbiological indices adequately reflect the effects of chitosan-iron nanocomposites in the process of evaluating the action of nanocomposites obtained by different processes on the representative yeast Rh. gracilis CNMN-Y-30 and it is recommended to test the degree of influence of the nanocomposite. This information can be used by specialists in the food industry, microbiology, medicine, cosmetology, environmental protection, etc., where nanocomposites have applications

CAROTENOID BIOSYNTHESIS POTENTIAL AND MORPHOLOGICAL CHARACTERS OF Rhodotorula gracilis YEAST UNDER THE ACTION OF FE3O4 NANOPARTICLES

This paper presents the results of the influence of Fe3O4 nanoparticles with dimensions of 10 nm in different con­centrations on the biosynthesis potential of carotenoid pigments and morphological characters of strain of  Rhodotorula gracilis CNMN-Y-30 yeast strain. Fe3O4 (10 nm) nanoparticles in concentrations of 0.5 to 15 mg/L have been found to induce significant changes in carotenoid formation and morphological carbohydrate formation in the yeast strain expressed by decreasing the amount of carotenoids in cell biomass and cell sizes. The correlation between the amount of carotenoids and the cell range is strong. The results obtained can contribute to further research in order to develop the theoretical and practical bases for the use of Fe3O4 nanoparticles in nanobiotechnologies.POTENȚIALUL DE BIOSINTEZĂ AL CAROTENOIDELOR ȘI CARACTERELE MORFOLOGICE ALE LEVURII Rhodotorula gracilis SUB ACȚIUNEA NANOPARTICULELOR FE3O4În lucrare sunt prezentate rezultatele influenței nanoparticulelor Fe3O4 cu dimensiuni de 10 nm, în diferite concentrații, asupra potențialului de biosinteză al pigmenților carotenoizi și caracterelor morfologice ale tulpinii de levuri Rhodotorula gracilis CNMN-Y-30. S-a constatat că nanoparticulele Fe3O4 (10 nm) în concentrații de la 0,5 până la 15 mg/L induc modificări semnificative ale capacității de formare a carotenoidelor și caracterelor morfologice la tulpina de levuri, expri­mate prin micșorarea cantității de carotenoide în biomasa celulară și a dimensiunilor celulelor. Raportul co­relațional dintre cantitatea de carotenoide și aria celulelor este unul puternic. Rezultatele obținute pot contribui la cer­cetă­rile ulterioare în scopul dezvoltării bazelor teoretice și practice de utilizare a nanoparticulelor Fe3O4 în nanobio­tehnologii.</p

THE EFFECT OF ZnO NANOPARTICLES ON THE ACTIVITY OF ANTIOXIDANT ENZYMES AND CAROTENOID CONTENT AT RHODOSPORIDIUM TORULOIDES CNMN-Y-30 YEAST

The present research paper provides new information on the influence of ZnO nanoparticles (ZnO NPs) of size &lt;50 nm and &lt;100 nm on Rhodosporidium toruloides CNMN-Y-30 pigmented yeast. It was established that the activity of antioxidant enzymes such as catalase, superoxide dismutase and content of carotenoid pigments in the studied strain has been modified depending on the size and concentrations of NPs. There were no significant differences between the activity of antioxidant enzymes and content of carotenoid pigments in experimental group and control at the use of significantly low concentration of ZnO NPs. The use of nanoparticles in concentration of 30 mg/l caused a decrease in activity of antioxidant enzyme catalase and contributed to the increase in the activity of superoxide dismutase. This study has revealed that the concentration of 30 mg/L of ZnO NPs initiates an significant decrease in the content of carotenoid pigments - β-carotene, torulene and torularhodin in cell biomass. The results provided opportunities for modeling cell cycle processes and highlighting of carotenoid pigments and antioxidant enzymes as parameters for determining the mode of action of nanoparticles. &nbsp

Biosynthesis of β-glucans and morphological features Saccharomyces cerevisiae CNMN-Y-20 yeasts under the action of zinc oxide nanoparticles

The paper provides new information on β-glucans biosynthesis capacity and the morphological features modification of cells and colonies of Saccharomyces cerevisiae CNMN-Y-20 yeast strain known as producer of β-glucans. It was found that the development cycle and bioproductive capacity of the yeast was affected by cultivation in the presence of ZnO in a concentrations and the contact duration manner. Within 6-24 hours, the reproduction of the nanomodified cell was decreased compared to the control, but after 120 hours of submerged cultivation an insignificant increase in biomass content relative to 1L culture medium was recorded predominantly related to nanoparticle concentrations 0.5-1.0 mg/L. ZnO nanoparticles (<100 nm). Concentrations of 0.5-15 mg/L initiated a 13-15% increase in average dimensions of Saccharomyces cerevisiae CNMN-Y-20 cells and 7-12% increase in the ability to form of β-glucans, especially at concentrations of 1 and 5 mg/L. The correlation coefficient between the cells area and the β-glucans amount is strong (R² = 0.8021). The results provide the possibility of enhancing of the range of analyzes and the formation of reference bases necessary for the strategy to enlarge the biotechnological performance of yeasts

INFLUENŢA NANOPARTICULELOR TiO2 ŞI Fe3O4 ASUPRA CREŞTERII ŞI COMPOZIŢIEI BIOCHIMICE A TULPINII DE LEVURI RHODOTORULA GRACILIS CNMN-Y-30

În lucrare sunt prezentate rezultatele cercetării privind influenţa nanoparticulelor anorganice TiO2 şi Fe3O4 cu dimen­­siu­nea de 30 nm, în diferite concentraţii, asupra procesului de creştere şi componenţei biochimice a biomasei tulpinii de levuri Rhodotorula gracilis CNMN-Y-30. S-a constat că nanoparticulele în concentraţii de 0,5 până la 5,0 mg/L nu modi­fică în mod semnificativ procesul de reproducere a celulelor şi productivitatea levurii. Efectul pozitiv se mani­festă prin mo­di­ficări favorabile ale conţinutului de carbohidraţi şi proteine la cultivare în prezenţa nanoparticulelor TiO2 şi Fe3O4. INFLUENCE NANOPARTICLES Fe3O4 AND TiO2 ON GROWTH AND BIOCHEMICAL COMPOSITION OF YEAST STRAIN RHODOTORULA GRACILIS CNMN-Y-30The present work provides the results of the research of the influence of the inorganic nanoparticles TiO2 and Fe3O4 with the size of 30 nm, in different concentrations, on the growth process and biochemical composition of biomass stalk of the yeast Rhodotorula gracilis CNMN-Y-30. It has been found that nanoparticles at concentrations of 0.5 to 5.0 mg/L did not significantly alter the reproduction of the cells and the productivity of the yeast. The positive effect is manifested by favorable changes in carbohydrate and protein content, at the cultivation in the presence of the nanoparticles TiO2 and Fe3O4. </p

OPTIMIZATION OF THE PROCESS FOR OBTAINING ANTIOXIDANT EXTRACTS RICH IN β-CAROTENE FROM THE RESIDUAL BIOMASS OF ARTHROSPIRA PLATENSIS

This study aimed to optimize the process of obtaining antioxidant extracts rich in β-carotene from the remaining biomass of Arthrospira platensis. Optimal extraction procedures using ultrasound, homogenization and temperature were investigated at the laboratory level. The results demonstrated that the highest yield of β-carotene in the extracts was obtained using 96 % ethylic alcohol at a temperature of +45 °C for 30 minutes and a biomass/solvent ratio of 1 : 50 g·mL-1 and 1 : 10 g·mL-1 . The activity of the antioxidant enzymes catalase and superoxide dismutase varied depending on the extraction method, and the highest activity was obtained in the experimental variants that were placed at the temperature of +35 °C and +45 °C. Considering the valuable biochemical composition and enzymatic activity, the antioxidant extracts obtained have a relevant potential for implementation in various fields such as animal husbandry, medicine and food