Studying the potential of xanthan gum production by local bacterial isolate of Iran

Background and Aim: Xanthan gum is a polysaccharide biopolymer which is produced by bacteria of the genus Xanthomonas. The purpose of this study is evaluation of  increase potential of xanthan gum production by local bacterial isolate from Iran. Materials and Methods: In this study, xanthan production in a new strain and native of Xanthomonas campestris strain saba.ton and bacterium of X. campestris PTCC 1473.To produce the gum, bacteria after becoming active in YDC Broth and producing have been studied biomass to were transferred  Gum (Production Culture). To produce to a sufficient a mount cell dry weight, diluted fermentation medium and centrifuged, the supernatant was removed for isolation of xanthan cell mass deposition, resuspension and isolated by ethanol precipitation of xanthan and xanthan dry weight was determined by centrifugation done. Results: Based on comparison between native species with standard species, the native one has had a faster growth (within 24 hours of native strains, reference strains during 48 hours). The Colonies of native species in comparison with standard strains were more yellow, larger and more highly viscous but two bacteria have had the same biochemical properties. Dry weight of xanthan produced by the native strain was 1.08 g /100 ml and standard strain was 0.73 g /100 ml. Conclusions: The results showed that the bacterium of X. campestris strain saba.ton as a native strain, in comparison with standard strain of X. campestris than PTCC 1473 without optimization in conditions of growth have high in the production of xanthon and it can be used in different industries with purifying produced xanthan

Identification of Microbial Agents Indifferent Parts of the Gastric Juice Company

Background and Aim: The main principle of ensuring the health and pharmaceutical medicine.In accordance with its combination of drugs can be the perfect environment for growth of microorganisms.The aim this research was to identify microorganisms in raw materials, water and environment, and other antimicrobial disinfectant effect of in eliminating them. Materials and Methods: In this descriptive study, 5 step 52 series production of gastric juice manufacturing factory sampling was performed before and after sterilization.Plate count method was used to measure microorganisms. If there is contamination of the culture media and biochemical tests for the detection of specific microorganisms such as Staphylococcus aureus, E.coli, Pseudomonas aeruginosa, Aspergillus niger and Salmonella spp. were used. Results: The result showed that Micrococcus in 3 cases, 2 cases of Serratia spp., Bacillus subtilis, in two instances, in one instance, Salmonella spp., S. aureus and A.niger in one sample were present in one sample. Conclusions: The experiments showed that the infected samples 19.2% are. 13.5%, which includes microorganisms, non-pathogenic, with over 100 CFU/ml are 5/7% of the sample remained, including microorganisms, virulence such as S. aureus, 1.1%, Salmonella 1.1% the A.niger and 1.1% respectively

Identification of Microbial Agents Indifferent Parts of the Gastric Juice Company

Background and Aim: The main principle of ensuring the health and pharmaceutical medicine.In accordance with its combination of drugs can be the perfect environment for growth of microorganisms.The aim this research was to identify microorganisms in raw materials, water and environment, and other antimicrobial disinfectant effect of in eliminating them. Materials and Methods: In this descriptive study, 5 step 52 series production of gastric juice manufacturing factory sampling was performed before and after sterilization.Plate count method was used to measure microorganisms. If there is contamination of the culture media and biochemical tests for the detection of specific microorganisms such as Staphylococcus aureus, E.coli, Pseudomonas aeruginosa, Aspergillus niger and Salmonella spp. were used. Results: The result showed that Micrococcus in 3 cases, 2 cases of Serratia spp., Bacillus subtilis, in two instances, in one instance, Salmonella spp., S. aureus and A.niger in one sample were present in one sample. Conclusions: The experiments showed that the infected samples 19.2% are. 13.5%, which includes microorganisms, non-pathogenic, with over 100 CFU/ml are 5/7% of the sample remained, including microorganisms, virulence such as S. aureus, 1.1%, Salmonella 1.1% the A.niger and 1.1% respectively