Imunohistokemijski dokaz antigena goveđeg herpevirusa 1 i goveđeg adenovirusa 3 u smrznutim i parafiniranim rezovima pluća u pneumoničnih ovnova i jaraca uzgojenih za klanje.

The aim of this study was to determine Bovine Adenovirus type 3 (BAV3) and Bovine Herpesvirus type 1 (BHV1) antigens in frozen and formalin-fixed paraffin-embedded lung tissues of pneumonic sheep and goats, using direct fluorescent a ntibody technique (DFAT) and immunoperoxidase (IP ) staining. For this purpose, the lungs of 1276 male sheep and 1087 male goats, which were raised in various farms in the Elazig province and surrounding areas and were brought to the abattoir for slaughtering between January and December 2011, were examined. Macroscopic pneumonia findings were detected only in the apical and cardiac lobes of the lungs of 132 sheep and 145 goats. The rates of mild, moderate and severe consolidations observed in the apical and cardiac lobes of pneumonic lungs were 57.5 %, 29.5 % and 12.8 % for sheep, respectively and 62.0 %, 20.0 % and 17.9 % for goats, respectively. Pneumonias were microscopically classified in sheep and goats as catarrhalpurulent (40.1 % and 33.1 %), interstitial (26.5 % and 31.7 %), fibrinous (21.2 % and 26.9 %), verminous (9.4 % and 6.2 %) and pulmonary adenomatosis (1.5 % and 2.0 %). In the examination with IP and DFAT in terms of BAV3 and BHV1 antige ns, a total of 249 (116 sheep and 133 goats) pneumonic lungs, but not verminous and pulmonary adenomatosis, were considered. BAV3 and BHV1 antigens were determined to be 5.1 % and 1.7 % in sheep and 0.7 % and 2.2 % in goats by the IP method, respectively. BAV3 and BHV1 antigens were found to be 9.4 % and 5.1 % in sheep and 4.5 % and 7.5 % in goats by DFAT, respectively. In conclusion, the presence of viral antigens in lung tissues of sheep and goats may indicate that natural pneumonia may be induced by BAV3 and BHV1 or possibly other species-specific adenoviruses and herpesviruses. In addition, it is thought that sheep and goats might have a role in the transmission of these viruses to cattle.Cilj ovog istraživanja bio je dokazati antigene goveđeg adenovirusa 3 i goveđeg herpesvirusa 1 izravnom imunofluorescencijom i imunoperoksidaznim testom u smrznutom i formalinom fiksiranom te u parafin uklopljenom tkivu pluća pneumoničnih ovnova i jaraca uzgojenih za klanje. Pretraženo je tkivo pluća od 1276 ovnova i 1087 jaraca, uzgajanih na različitim farmama na području Elaziga i okolice te dovedenih na klanje između siječnja i prosinca 2011. Upala pluća dokazana je makroskopski samo u apikalnim i kardijalnim režnjevima 132 ovna i 145 jaraca. Blaga konsolidacija dokazana je u 57,5 %, srednje jaka u 29,5 % te jaka u 12,8 % ovnova, dok je u 62,0 % jaraca dokazana blaga, 20,0 % srednje jaka te 17,9 % jaka konsolidacija plućnog tkiva. Upala pluća bila je mikroskopski dijagnosticirana kao kataralno-purulentna u 40,1 % ovnova i 33,1 % jaraca, intersticijska u 26,5 % ovnova i 31,7 % jaraca, fibrinozna u 21,2 % ovnova i 26,9 % jaraca, verminozna u 9,4 % ovnova i 6,2 % jaraca te kao plućna adenomatoza u 1,5 % ovnova i 2,0 % jaraca. Imunoperoksidaznim i izravnim fluorescentnim testom na prisutnost antigena za goveđi adenovirus 3 i goveđi herpesvirus 1 bilo je ukupno pretraženo 249 (116 od ovnova i 133 od jaraca) pneumoničnih pluća. U tu brojku nije bila uključena verminozna pneumonija ni plućna adenomatoza. Antigeni za goveđi adenovirus 3 bili su dokazani imunoperoksidaznim testom u 5,1 % ovnova i 0,7 % jaraca, dok su antigeni za goveđi herpesvirus 1 bili dokazani u 1,7 % ovnova i 2,2 % jaraca. Izravnim imunofluorescentnim testom antigeni za goveđi adenovirus 3 bili su dokazani u 9,4 % ovnova i 4,5 % jaraca, dok su antigeni za goveđi herpesvirus 1 bili dokazani u 5,1 % ovnova i 7,5 % jaraca. Prisutnost virusnih antigena u plućnom tkivu ovaca i koza može upućivati na zaključak da u njih upala pluća u terenskim uvjetima može biti uzrokovana goveđim adenovirusom 3 i goveđim herpesvirusom 1 ili nekim drugim adenovirusima i herpesvirusima specifičnima za vrstu. Smatra se da ovce i koze mogu imati određenu ulogu u prijenosu tih virusa na govedo