Specific recognition of a multiply phosphorylated motif in the DNA repair scaffold XRCC1 by the FHA domain of human PNK.

Short-patch repair of DNA single-strand breaks and gaps (SSB) is coordinated by XRCC1, a scaffold protein that recruits the DNA polymerase and DNA ligase required for filling and sealing the damaged strand. XRCC1 can also recruit end-processing enzymes, such as PNK (polynucleotide kinase 3'-phosphatase), Aprataxin and APLF (aprataxin/PNK-like factor), which ensure the availability of a free 3'-hydroxyl on one side of the gap, and a 5'-phosphate group on the other, for the polymerase and ligase reactions respectively. PNK binds to a phosphorylated segment of XRCC1 (between its two C-terminal BRCT domains) via its Forkhead-associated (FHA) domain. We show here, contrary to previous studies, that the FHA domain of PNK binds specifically, and with high affinity to a multiply phosphorylated motif in XRCC1 containing a pSer-pThr dipeptide, and forms a 2:1 PNK:XRCC1 complex. The high-resolution crystal structure of a PNK-FHA-XRCC1 phosphopeptide complex reveals the basis for this unusual bis-phosphopeptide recognition, which is probably a common feature of the known XRCC1-associating end-processing enzymes

INFLUENCE OF COMPOST, MINERAL AND EFFECTIVE MICROORGANISMS APPLICATION ON SANDY SOIL-GROWN BERMUDA TURFGRASS

Two field experiments were conducted during 2016 and 2017 seasons at the Experimental Farm, Fac. Agric., Minia Univ. to study the effect of compost, mineral NPK and/or Effective microorganisms (E.M.) on the performance and chemical constituents of bermuda grass grown in sandy soil. Obtained results revealed that compost, especially when added at the high level (22.5 ton/fed), as well as, 75 % NPK + E.M. caused considerable increase in all studied vegetative growth characters, as well as, the three photosynthetic pigments and N, P and K %. However, the high compost level (22.5 ton/fed) in combination with the dual fertilization treatment (75 % NPK + E.M.) resulted in the highest overall grown and the best performance of bermuda turf grass grown in sandy soil

Supersymmetric Effects on Isospin Symmetry Breaking and Direct CP Violation in B→ργB \to \rho \gamma

We argue that one can search for physics beyond the standard model through measurements of the isospin-violating quantity $\Delta^{-0} \equiv \Gamma(B^- \to \rho^- \gamma)/2\Gamma(B^0 \to \rho^0 \gamma)-1$, its charge conjugate $\Delta^{+0}$, and direct CP violation in the partial decay rates of $B^\pm \to \rho^\pm \gamma$. We illustrate this by working out theoretical profiles of the charge-conjugate averaged ratio $\Delta \equiv {1 \over 2}(\Delta^{+0} +\Delta^{-0})$ and the CP asymmetry $A_{CP}(B^\pm \to \rho^\pm \gamma)$ in the standard model and in some variants of the minimal supersymmetric standard model. We find that chargino contributions in the large $\tan \beta$ region may modify the magnitudes and flip the signs of $\Delta$ and $A_{CP}(B^\pm \to \rho^\pm \gamma)$ compared to their standard-model values, providing an unmistakeable signature of supersymmetry.Comment: 10 pages, 7 figures (requires graphicx

Double Penguins and the Contribution of Vector Meson--like States to the Decays B→K∗γ, B→ργB \to K^* \gamma, \, B \to \rho \gamma

Using perturbative QCD, the contribution at the leading twist, leading $\alpha_s$ level, of charm and up quark loops to the decays $B \rightarrow K^* \gamma$ and $B \rightarrow \rho \gamma$ is presented. In the case of $B \rightarrow \rho \gamma$, the relative importance of these contributions depend upon the unknown CKM matrix elements $V_{bu}$ and $V_{td}$. Assuming that the ratio $r = V_{bc}V^*_{cd}/V_{bt}V^*_{td}$ is bounded between $-2.25 \le r \le -.5$ as is suggested by the Particle Data Group, the error in extracting $|V_{td}/V_{ts}|$ by these decays is estimated.Comment: 4 double column pages, revtex, 3 figures attached, 2 figures added indicating less optimism concerning the accuracy at which $|V_{td}/V_{ts}|$ can be extracted: to appear in PR

Examination of the Resonance Contributions to Dileptonic Rare B-Decays

We analyse the long-distance contribution to $B\to X_s\ell^+\ell^-$ differential decay rate when the momentum dependence of $\psi$ and $\psi'$-$\gamma$ conversion strength is taken into account. The results indicate that the resonance to nonresonance interference in the dilepton invariant mass distribution is substantially reduced.Comment: 10 pages, Latex, one figure (included

Exclusive Radiative B-Decays in the Light-Cone QCD Sum Rule Approach

We carry out a detailed study of exclusive radiative rare $B$-decays in the framework of the QCD sum rules on the light cone, which combines the traditional QCD sum rule technique with the description of final state vector mesons in terms of the light-cone wave functions of increasing twist. The decays considered are: $B_{u,d} \to K^* +\gamma, B_{u,d}\to \rho+\gamma, B_d\to \omega+\gamma$ and the corresponding decays of the $B_s$ mesons, $B_s\to \phi+\gamma$ and $B_s\to K^*+\gamma$. Based on our estimate of the transition form factor F_1^{B \to K^*\pg}(0) =0.32\pm0.05, we find for the branching ratio $BR(B \to K^* + \gamma) = (4.8\pm 1.5)\times 10^{-5}$, which is in agreement with the observed value measured by the CLEO collaboration. We present detailed estimates for the ratios of the radiative decay form factors, which are then used to predict the rates for the exclusive radiative B-decays listed above. This in principle allows the extraction of the CKM matrix element $|V_{td}|$ from the penguin-dominated CKM-suppressed radiative decays when they are measured. We give a detailed discussion of the dependence of the form factors on the $b$-quark mass and on the momentum transfer, as well as their interrelation with the CKM-suppressed semileptonic decay form factors in $B\to \rho+\ell+\nu$, which we also calculate in our approach.Comment: 32 pages, 10 uuencoded figures, LaTeX, preprint CERN-TH 7118/9

Calculation of two-loop virtual corrections to b --> s l+ l- in the standard model

We present in detail the calculation of the virtual O(alpha_s) corrections to the inclusive semi-leptonic rare decay b --> s l+ l-. We also include those O(alpha_s) bremsstrahlung contributions which cancel the infrared and mass singularities showing up in the virtual corrections. In order to avoid large resonant contributions, we restrict the invariant mass squared s of the lepton pair to the range 0.05 < s/mb^2 < 0.25. The analytic results are represented as expansions in the small parameters s/mb^2, z = mc^2/mb^2 and s/(4 mc^2). The new contributions drastically reduce the renormalization scale dependence of the decay spectrum. For the corresponding branching ratio (restricted to the above s-range) the renormalization scale uncertainty gets reduced from +/-13% to +/-6.5%.Comment: 41 pages including 9 postscript figures; in version 2 some typos and inconsistent notation correcte

Using b→sγb \to s\gamma to Probe Top Quark Couplings

Possible anomalous couplings of the top-quark to on-shell photons and gluons are constrained by the recent results of the CLEO Collaboration on both inclusive and exclusive radiative $B$ decays. We find that the process \bsg\ can lead to reasonable bounds on both the anomalous electric and magnetic dipole moments of the top-quark, while essentially no limits are obtained on the corresponding chromoelectric and chromomagnetic moments, which enter the expression for the decay rate only through operator mixing.Comment: 10 pages plus 6 figures (available by request), LaTex, ANL-HEP-PR-93-3

Analysis of Charge Asymmetry in Rare Dilepton BB Decays

We analyze forward-backward charge asymmetry of the lepton production in rare decays $B\rightarrow X_s l^+l^-$ and $B\rightarrow K^* l^+l^-$, including vector-resonance effects. Certain regions of phase space, in which the asymmetry is sensitive to individual short-distance coefficients, are pointed out. In particular, we suggest a method to test the coupling of the leptonic axial vector current to the left-handed quark current experimentally.Comment: 27 pages, 4 figures available up to requiremen