In Silico Screening of Sesquiterpene Lactones as Aldose Reductase Inhibitors

Aldose reductase, a key enzyme of the polyol pathway catalyses NADPH-dependent reduction of glucose to sorbitol. Increased activity of this enzyme is considered a major factor contributing to the development of diabetic complications hence could be an important target in the treatment of these complications. In this work, a database of sesquiterpenes was prepared and screened for their drug-like properties based on the Lipinski’s rule of 5. The co-crystallised structure of aldose reductase was obtained from the Protein Databank and prepared for docking. In silico docking experiments was performed on Autodock tools using 198 sesquiterpene lactones that passed screening, and compounds with the lowest binding energy and favourable binding interactions were selected for molecular docking simulation. Six of the best ranking compounds selected had binding energies ranging from–11.96 Kcal/mol to -9.45 Kcal/mol  and were comparable to the energy of the standard inhibitor Idd594 used in the study. They also show good complementarity in their binding to the residues of the binding pocket. The results suggest that dehydrooopodin (1), 11(S),13-dihydrolactucopicrin (2), and Chrysanin (3) offered potential inhibitory activities toward aldose reductase and may serve as lead compounds for in vivo validation as aldose reductase inhibitors. Keywords: Sesquiterpene lactones, Aldose reductase, Binding energy, Molecular docking, Autodoc

Small Earth Dams in Nigeria: Prospects and Challenges

The study investigates the challenges and prospects of earth dams in Nigeria with emphasis on dams owned by the River Basin Development Authorities of the Federal Ministry of Agriculture and Water resources using Cham dam in Gombe State as case study. The general causes of earth dam failure were considered with emphasis on failures due to engineering factors. The dam water provides raw water for the supply of potable wholesome water to the community all year round and some for irrigated agriculture that not only boost the local economy and reduces poverty but also support a viable livestock management. Cham dam failed in September 1998 after commissioning in December 1992 as a result of poor planning, inadequate study, inconsistent design, un-engineered construction and lack of dam safety monitoring team at site. Based on the findings, suggestions were given on how some earth dam failures in Nigeria can be minimized in terms of adequate feasibility studies, good design, appropriate construction and an improved maintenance culture. Keywords: Small earth dams, un-engineered construction, environmental management, prospects SED, hydraulic failure. DOI: 10.7176/JETP/9-5-02 Publication date:June 30th 201

Expression of Wild-Type p53 by Curcumin, Alpinetin and Flavokawain B in Colorectal Cancer cells Expressing R273H Mutant p53

A mutation in p53 is frequently reported in nearly 50% of all of human cancers arising from DNA-binding core domain of p53. DNA-contact mutant R273H rendered p53 at dysfunctional state due to the substitution of single residue Arg273 for His273. Here, natural bioactive compounds curcumin, alpinetin and flavokawain B were investigated for possible stabilisation of wild-type p53 expression in vitro using HT-29 cells harbouring R273H rendered p53. Accordingly, all the bioactive compounds were able to induce the expression of wild-type p53 both at the levels of gene and protein expression. A dose-dependent induction of p53 was evident at 12.5, 25 and 50 μM concentration. The present study has shown that the bioactive compounds may have restored the wild-type p53 functional activity in tumour cells expressing R273H mutant p53. Keywords: Curcumin, Alpinetin, Flavokawain B, p53, R273

Identification of Lubricating Oil-Degrading Microorganisms in Oil Polluted Soils from Five Auto- mechanic Workshops in Accra, Ghana

Trichothecium, Trichoderma, Aspergillus niger, Fusarium, and Penicillium spp. from oil contaminated soil from 5 Mechanic Shops in Accra, Ghana showed zones of clearance of oil on Minimum Salt Enrichment Medium (MSEM) Agar seeded with 1000ppm Engen™ Lubricating Oil (ELO), so were counted as presumptive lubricating oil-utilizing moulds. Significant increases (P ? 0.05) in viable counts, fungal dry weights and optical densities; significant decreases (P ? 0.05) in pH’s of pure cultures of the moulds in MSEM+1.0%(v/v) ELO medium at 30°C for 0 - 25 day’s; positive correlations between viable counts and fungal dry weights, viable counts and optical densities, and fungal dry weights and optical densities; and negative correlations between pH and viable counts, and pH and optical densities, confirmed the moulds as lubricating oil consuming fungi with potential for use in bioremediation of oil polluted soils. Aspergillus niger exhibited the highest bioremediation capacity and Trichothecium the least. Keywords: Lubricating Oil, Pollution, Fungi, Bioremediatio

Assessing Municipal Solid Waste Management Practices and Challenges in the Techiman Municipality, Ghana

Managing waste efficiently is essential for building sustainable, livable and healthy communities but this remains a challenge for many municipal governments due to limited municipal budget and other logistical challenges. Such challenges result in ineffective waste collection and disposal. However, identifying the challenges associated with municipal solid waste management often lead to developing solutions to mitigate the problem. This paper assesses the waste management practices and challenges within the Techiman municipality, the regional capital of the Bono East Region. By sampling residents’ perceptions and experiences it was observed that households and patrons were dissatisfied with Techiman’s Municipal Solid Waste Management (MSWM). Statistically the per capita per day rate of waste generation was 0.48kg/ per capita/per day, being higher than Ghana’s municipal waste generation of 0.40 kg/per capita/per day. Lack of collection of waste from the transfer stations to the landfill sites has resulted in about 67 heaps of uncollected waste in the municipality. The results show that lack of source separation and recycling, broken down trucks, low participation of private sector in waste collection, non-compliance of by-laws, poor road infrastructure leading to the landfill site, and inefficient landfill site have contributed to the waste problem in the municipality. Resorting to source separation, educating the public on waste management bye laws, increasing private sector participation and establishing engineered landfill sites can substantially contribute to sustainable Municipal Waste Management in the Techiman Municipality

Effect of ascorbic acid on behaviour of Wistar rats exposed to environmental heat stress

Introduction: The hot-dry season in the Northern Guinea Savannah zone of Nigeria is characterized by high ambient temperature and high relative humidity which imposes heat stress on the body resulting in enormous generation of free radicals and behavioural changes. Aim: The aim of the study was to determine the effect of ascorbic acid (AA) on behavior of Wistar rats exposed to environmental in heat stress. Methods: Thirty Wistar rats weighing 140-160g were divided into 3 groups of 10 each. Group 1 served as control and was administered normal saline 1ml/kg orally, groups 2 exposed to environmental temperature of 38 ± 1 0C and group 3 were exposed to environmental temperature of 38 ± 1 0C and administered AA at 100mg/kg orally for 21 days. Behavioral activity in the open-field was assessed on days 1, 7, 14 and 21. Results: The results showed a significant increase in pellets count, urination and immobilization in the control and heat exposed group compared to AA treated on days 1 and 7. Rearing, grooming and ambulation were significantly decreased in AA treated group on day 1, 7 and 14. There was no significant behavioral change on the 21st day indicating habituation. Conclusion: It was concluded that ascorbic acid ameliorated some of the behavioral changes induced by environmental heat stress and therefore, individuals working in high temperature zones should be advised to be taking vitamin C supplements

Heavy Metal Content of Agricultural Soils in a Tropical Sudan Savannah Area: Katsina State, North-Western Nigeria

This work contributes to the monitoring of Agricultural soil pollution in Katsina State North western Nigeria by assessing the degree of heavy metal pollution in Agricultural soil samples The study was conducted in the year 2017 within some catchment areas located within the 3 senatorial zones that constitute to make up the state Katsina senatorial zone Birchi Dutsinma and Katsina Daura senatorial zone Daura Ingawa and Zango Funtua senatorial zone Dabai Funtua Kafur Malunfashi and Matazu Analysis for the concentration of these heavy metals Cr Cd Fe Ni Mn Pb and Zn was conducted by the use of AAS by Atomic Absorption Spectrophotometry method Several indices were used to assess the metal contamination levels in the Agricultural soil samples namely Geo-accumulation Index Igeo Enrichment Factor EF Contamination Factor CF Degree of Contamination Cd and Pollution Load Index PL

Factors Influencing Farmers’ Awareness and Choice of Indigenous Practices in Adapting to Climate Change and Variability in Northern Ghana

Farmers in Northern Ghana adapt to the adverse effects of climate change using traditionalpractices. However, farmers’ awareness of these practices is a spring-board to their choicesof available indigenous climate related adaptation strategies. This study examined farmers’awareness and usage of indigenous adaptation strategies using the Heckman Two-Stage Sample Selection Model. Questionnaire was administered to 285 randomly selected households to determine the factors influencing farmers’ awareness and choice of indigenous adaptation strategies. The empirical results showed that majority of farmers are aware of and employed soil related indigenous adaptation strategies. Education, membership of farmer-based organisation, farmer-farmer extension contacts and farming experience significantly determine farmers’ awareness of indigenous adaptation strategies. Farmers’ level of education, farming experience, farmer-farmer extension contacts, membership of farmer group, labour hours and age significantly influenced farmers’ choice of indigenous climate related adaptation strategies.Based on these findings, it is recommended that farmers should endeavour to form groups toserve as a platform to share knowledge on indigenous farming practices for effective climatechange adaptation

Determination of Some Heavy Metals in Soil Samples Obtained From Rimi Local Government in Katsina State, Nigeria

Abstract: The concentration of lead, chromium, iron, zinc, potassium, magnesium and sodium in soil samples obtained from Rimi local government in Katsina state was analyzed using Atomic Absorption Spectroscopy Technique. The Highest Mean concentration of   the metals for each sample were as follows:  Lead (8.3 ± 0.000 mg/g), Chromium (3.125±4.4.42 mg/g), Iron (14.2±0.000 mg/g), Zinc (4.2 ± 0.000 mg/g), Potassium (218.7 ± 44.19 mg/g), Magnesium (68.75 ± 8.84 mg/g)  and Sodium (1446.45 ± 25.24 mg/g), The results were discussed in terms of effects of the concentration of these metals on the agricultural soil understudy and the concentration obtained was below the ECDG (2004), WHO (2008), Kabata-Pendias (1995), SEPA (2000) and  DPR (1991) Permissible limits of heavy metals in Agricultural land. Although it was concluded that the soil is suitable for agricultural purposes, Remediation of heavy metal contaminated soils is necessary to reduce the associated risks, make the land resource available for agricultural production, enhance food security and scale down land tenure problems arising from changes in the land use pattern. Keyword: Agricultural Soil Pollution, Trace and Heavy Metals, Environmental Pollution