Isolation, Characterization, Distribution and Antifungal Sensitivity of Candida albicans from Oral Cancer Patients

The aim of this study was to investigate the prevalence of oral candidiasis in cancer patients and their antifungal susceptibility. A total of 50 cancer patients were included in this study, oral examination was done and oral swabs were taken from all the participants for yeast culture, identification and susceptibility testing to Fluconazole and two volatile oils by agar test. Oral candidiasis was prevalent in 23 (46%) of all cancer patients. The highest rate of total candidiasis was seen in male 12 (52.2%) than female 11 (47.8%). The highest prevalence of Candida albicans was in the age group 41-60 years which was 13 (56.52%) while the lowest was in children 2(8.96%). The overall sensitivity of C. albicans to Fluconazole and Clove oil was 100% and 99% respectively, and no effect of coriander oil was found. The study revealed that the oral candidiasis was prevalent in patients who use chronic antibiotics, chewing qat, and tobacco smoking

Airborne Bacterial Pollution in Clinical Environment, Sana'a - Yemen

Abstract: This work aims to know the extent of air pollution and common bacterial species in delivery rooms in some hospitals in Sana'a city. Six randomly airborne samples from delivery rooms of three hospitals (Algmhory, Althwrah and Alsabeen hospitals). Out of 65 airborne bacteria were isolated from delivery rooms, 89.2% of them showed Gram positive bacterial isolates and 10.8% Gram negative bacteria. Bacteria isolates were identified as Staphylococcus aureus, Staphylococcus epidemidis, Micrococcus sp., Bacillus sp., Pseudomonas aeruginosa, Corynebacterium sp., Klebsiella sp., Lactobacillus sp. and Proteus mirabilis. The highest percentage of bacterial isolates was recorded in Alsabeen hospital as 66.2%. Staphylococcus was the most dominant organism isolated from the delivery rooms in all examined hospitals (37 isolates, 56.9%), while the lowest percentages were: Lactobacillus (two isolates, 3.1%), Klebsiella and Proteus (one isolate, 1.5%) for each

Effect of Some Volatile Oils on Staphylococcus aureus and Pseudomonas aeruginosa Isolates from Burn Patients

Burn wound infection is a major complication in burn patients after the initial period of shock. Microbial infection of burn wounds causes more than 70% mortality in burn patients. This study was conducted from March 2019 to May 2019 at Teiba Center for Burns Surgery in Al-Jumhory Hospital located in Sana'a city, Yemen. A total of 40 swab specimens of burn wounds were collected. Specimens were inoculated on Blood agar media and MacConkey Agar media and were incubated aerobically for 24 hours at 37 °C. Specimens of third-degree burns were inoculated on Blood Agar media and incubated anaerobically for 75 hours at 37°C. Bacterial colonies were identified by biochemical and diagnostic tests. Screening of volatile oils (Lavandula pubescens, Plectranthus barbatus, and Thymus laevigatus) for antibacterial activities was done by the disk diffusion method. Our results showed that 25 (63%) of patients were males and 15 (37%) were females. The second-degree burns were the most common 18 (45%), followed by third-degree burns 13 (32%), 25 (62%) were flame, 7 (18%) were scalds, 8 (20%) cases were chemical burns. Out of 28 positive cultures, Staphylococcus aureus was the most common organism isolated 20 (71%), followed by Pseudomonas aeruginosa 8 (29%). Lavandula oils and Plectranthus oils showed inhibition against the growth of S. aureus and P. aeruginosa at 20µl

Efficacy of Volatile Oils against Salmonella Isolated from Gizzard in Poultry

Salmonella infection in poultry is one of the most important bacterial diseases which cause heavy economic loss through reduced production and mortality. Transmission of Salmonella is by direct contact with contaminated food of animal origins such as chicken meat and eggs. This study was conducted from April 2018 to May 2018 at store Ali Mohsen in Sana'a city, Yemen. A total of 60 samples of poultry gizzard were collected using a sterile sharp from store Ali Mohsen it was of Amran (20) samples, Thamar (10) samples, Hiziaz (20) samples, Al-hudaidah (10) samples and put it in sterile bottles. Salmonella were isolated from 40 (67%) gizzard samples while 20 (33%) gizzard samples were Negative. Positive samples were 14 (35%) from Amran, Hiziaz 12 (30%), Thamar 10 (25%) and 4 (10%) samples were positive from Alhudaidah. Volatile oils of Origanum majorana, Nepeta delflersina, and Mentha x piperita had antibacterial activities against Salmonella sp while isolated strains of Salmonella sp were resistant against volatile oils such as Coriandrum sativum, Elettaria cardamomum, Trachys permum and Artemi judaica sia. The volatile oil obtained from N. deflersiana contains sixteen identified and three unidentified constituents. The major constituents were 4a alpha, 7 alpha, 7a alpha-Nepetalactone (77.7%). The main chemical constituent in the volatile oil obtained from O. majorana was Terpinen-4-ol (35.2%). The main constituent of Mentha x piperita volatile oil was Limonene (7.9%). N. deflersiana, O.majorana, and M. x piperita volatile oils showed an inhibition zone against the growth of Salmonella isolates at 30µl