5 research outputs found

    ΠžΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΠΎΠ½Π½ΠΎ-мСтодичСскиС ΠΈ экономичСскиС основы создания ΡΠ΅Π»ΡŒΡΠΊΠΈΡ… ΠΊΡƒΡ€ΠΎΡ€Ρ‚ΠΎΠ² Π² Π³Π»ΡƒΠ±ΠΈΠ½Π½Ρ‹Ρ… тСрриториях Π³. Π‘ΠΎΡ‡ΠΈ

    Get PDF
    Π“ΠΎΡ€ΠΎΠ΄-ΠΊΡƒΡ€ΠΎΡ€Ρ‚ Π‘ΠΎΡ‡ΠΈ Π²ΠΊΠ»ΡŽΡ‡Π°Π΅Ρ‚ ΠΏΡ€ΠΈΠ±Ρ€Π΅ΠΆΠ½Ρ‹Π΅ ΠΈ Π³ΠΎΡ€Π½Ρ‹Π΅ ΠΊΡƒΡ€ΠΎΡ€Ρ‚Π½ΠΎ-туристскиС кластСры. ΠΠΊΡ‚ΡƒΠ°Π»ΡŒΠ½ΠΎΠΉ ΠΏΡ€ΠΎΠ±Π»Π΅ΠΌΠΎΠΉ Π² соврСмСнных условиях являСтся Π½Π΅ΠΎΠ±Ρ…ΠΎΠ΄ΠΈΠΌΠΎΡΡ‚ΡŒ вовлСчСния Π² ΠΈΠ½Π΄ΡƒΡΡ‚Ρ€ΠΈΡŽ Ρ‚ΡƒΡ€ΠΈΠ·ΠΌΠ° Π³ΠΎΡ€ΠΎΠ΄Π° ΠΏΡ€ΠΈΡ€ΠΎΠ΄Π½ΠΎ-Ρ€Π΅ΠΊΡ€Π΅Π°Ρ†ΠΈΠΎΠ½Π½Ρ‹Ρ… рСсурсов ΠΈ ΠΎΠ±ΡŠΠ΅ΠΊΡ‚ΠΎΠ² историко-ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€Π½ΠΎΠ³ΠΎ наслСдия Π΅Ρ‰Π΅ Π½Π΅ освоСнных Π³Π»ΡƒΠ±ΠΈΠ½Π½Ρ‹Ρ… Π½ΠΈΠ·ΠΊΠΎΠ³ΠΎΡ€Π½Ρ‹Ρ… Ρ‚Π΅Ρ€Ρ€ΠΈΡ‚ΠΎΡ€ΠΈΠΉ, ΠΈΠΌΠ΅ΡŽΡ‰ΠΈΡ… субтропичСскоС ΡΠ΅Π»ΡŒΡΠΊΠΎΡ…ΠΎΠ·ΡΠΉΡΡ‚Π²Π΅Π½Π½ΠΎΠ΅ Π½Π°Π·Π½Π°Ρ‡Π΅Π½ΠΈΠ΅. Π—Π°Π΄Π°Ρ‡Π° исслСдования β€” ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΠΈΡ‚ΡŒ Π½Π°ΡƒΡ‡Π½ΠΎ-мСтодичСскиС, ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΠΎΠ½Π½Ρ‹Π΅ ΠΈ финансовыС прСдпосылки для устойчивого развития этих Ρ‚Π΅Ρ€Ρ€ΠΈΡ‚ΠΎΡ€ΠΈΠΉ. Π’ исслСдовании сформулированы ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΠΎΠ½Π½Ρ‹Π΅, управлСнчСскиС ΠΈ Π·Π°ΠΊΠΎΠ½ΠΎΠ΄Π°Ρ‚Π΅Π»ΡŒΠ½Ρ‹Π΅ аспСкты основного противорСчия, ΠΊΠΎΡ‚ΠΎΡ€ΠΎΠ΅ связано с Ρ€Π΅ΡˆΠ΅Π½ΠΈΠ΅ΠΌ Π΄Π°Π½Π½Ρ‹Ρ… ΠΏΡ€ΠΎΠ±Π»Π΅ΠΌ: нСсоотвСтствиС ΠΏΡ€Π°Π²ΠΎΠ²ΠΎΠ³ΠΎ полоТСния ΠΈ статуса ΡΠ΅Π»ΡŒΡΠΊΠΈΡ… Ρ‚Π΅Ρ€Ρ€ΠΈΡ‚ΠΎΡ€ΠΈΠΉ Ρ€Π΅ΠΊΡ€Π΅Π°Ρ†ΠΈΠΎΠ½Π½Ρ‹ΠΌ Π·Π°Π΄Π°Ρ‡Π°ΠΌ ΠΊΡƒΡ€ΠΎΡ€Ρ‚Π½ΠΎΠΉ мСстности Π³. Π‘ΠΎΡ‡ΠΈ. Для прСодолСния этого противорСчия прСдлагаСтся совмСстноС Ρ€Π°Π·Π²ΠΈΡ‚ΠΈΠ΅ Π°Π³Ρ€Π°Ρ€Π½ΠΎΠΉ Π΄Π΅ΡΡ‚Π΅Π»ΡŒΠ½ΠΎΡΡ‚ΠΈ ΠΈ пСрспСктивных Π²ΠΈΠ΄ΠΎΠ² Ρ‚ΡƒΡ€ΠΈΠ·ΠΌΠ° ΠΈ Ρ€Π΅ΠΊΡ€Π΅Π°Ρ†ΠΈΠΈ Π² Π³Π»ΡƒΠ±ΠΈΠ½Π½Ρ‹Ρ… Π·ΠΎΠ½Π°Ρ… Π³ΠΎΡ€ΠΎΠ΄Π°, Ρ‚Π°ΠΊΠΈΡ… ΠΊΠ°ΠΊ Π»Π΅Ρ‡Π΅Π±Π½ΠΎ-ΠΎΠ·Π΄ΠΎΡ€ΠΎΠ²ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹ΠΉ, сСльский, этничСский ΠΈ Π½Π°ΡƒΡ‡Π½ΠΎ-ΠΏΠΎΠ·Π½Π°Π²Π°Ρ‚Π΅Π»ΡŒΠ½Ρ‹ΠΉ Ρ‚ΡƒΡ€ΠΈΠ·ΠΌ. Π‘ΠΎΡ†ΠΈΠ°Π»ΡŒΠ½ΠΎ-экономичСскоС влияниС развития Ρ‚ΡƒΡ€ΠΈΠ·ΠΌΠ° ΠΈ Ρ€Π΅ΠΊΡ€Π΅Π°Ρ†ΠΈΠΈ Π½Π° сСльской Ρ‚Π΅Ρ€Ρ€ΠΈΡ‚ΠΎΡ€ΠΈΠΈ ΠΊΡƒΡ€ΠΎΡ€Ρ‚Π° обСспСчит рост занятости ΠΈ ΠΏΡ€Π΅Π΄ΠΏΡ€ΠΈΠ½ΠΈΠΌΠ°Ρ‚Π΅Π»ΡŒΡΠΊΠΎΠΉ активности Π² связи с появлСниСм спСциализированной Ρ€Π΅ΠΊΡ€Π΅Π°Ρ†ΠΈΠΎΠ½Π½ΠΎΠΉ инфраструктуры, Π° Ρ‚Π°ΠΊΠΆΠ΅ сдСлаСт Π²ΠΎΠ·ΠΌΠΎΠΆΠ½Ρ‹ΠΌ ΠΏΠΎΠ²Ρ‹ΡˆΠ΅Π½ΠΈΠ΅ уровня ΠΆΠΈΠ·Π½ΠΈ мСстного Π°Π²Ρ‚ΠΎΡ…Ρ‚ΠΎΠ½Π½ΠΎΠ³ΠΎ насСлСния. Π‘ΠΎΠ·Π΄Π°Π½ΠΈΠ΅ ΡΠ΅Π»ΡŒΡΠΊΠΈΡ… ΠΊΡƒΡ€ΠΎΡ€Ρ‚ΠΎΠ² ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΡ‚ ΠΏΠΎΠ»Π½ΠΎΡ†Π΅Π½Π½ΠΎ ΠΈΡΠΏΠΎΠ»ΡŒΠ·ΠΎΠ²Π°Ρ‚ΡŒ Π±Π°Π»ΡŒΠ½Π΅ΠΎΠ»ΠΎΠ³ΠΈΡ‡Π΅ΡΠΊΠΈΠ΅ рСсурсы, ΠΏΡ€ΠΈΡ€ΠΎΠ΄Π½Ρ‹Π΅ Π»Π΅Ρ‡Π΅Π±Π½Ρ‹Π΅ Ρ„Π°ΠΊΡ‚ΠΎΡ€Ρ‹, ΡƒΠ½ΠΈΠΊΠ°Π»ΡŒΠ½ΠΎΠ΅ историко-ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€Π½ΠΎΠ΅ наслСдиС, ΠΏΡ€ΠΈΡ€ΠΎΠ΄Π½Ρ‹Π΅ Π»Π°Π½Π΄ΡˆΠ°Ρ„Ρ‚Ρ‹ ΠΈ возмоТности субтропичСского сСльского хозяйства Π² туристских цСлях. Π Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ‹ исслСдования Π΄Π°ΡŽΡ‚ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡ‚ΡŒ Ρ€Π°ΡΡΠΌΠ°Ρ‚Ρ€ΠΈΠ²Π°Ρ‚ΡŒ пространствСнноС Ρ€Π°Π·Π²ΠΈΡ‚ΠΈΠ΅ Ρ‚ΡƒΡ€ΠΈΠ·ΠΌΠ° Π½Π° ΠΊΡƒΡ€ΠΎΡ€Ρ‚Π΅, Ρ€Π°Π·Ρ€Π°Π±Π°Ρ‚Ρ‹Π²Π°Ρ‚ΡŒ ΠΈ Ρ€Π΅Π°Π»ΠΈΠ·ΠΎΠ²Π°Ρ‚ΡŒ обоснованныС ΠΏΡ€ΠΎΠ³Ρ€Π°ΠΌΠΌΡ‹ создания ΡΠ΅Π»ΡŒΡΠΊΠΈΡ… ΠΊΡƒΡ€ΠΎΡ€Ρ‚ΠΎΠ² ΠΈ туристских агродСстинаций. ΠŸΠΎΡΠ»Π΅Π΄ΡƒΡŽΡ‰ΠΈΠ΅ исслСдования Π±ΡƒΠ΄ΡƒΡ‚ посвящСны ΠΈΠ·ΡƒΡ‡Π΅Π½ΠΈΡŽ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½Ρ‹Ρ… способов трансформации рСсурсов ΡΠ΅Π»ΡŒΡΠΊΠΈΡ… Ρ‚Π΅Ρ€Ρ€ΠΈΡ‚ΠΎΡ€ΠΈΠΉ Π² Ρ„Π°ΠΊΡ‚ΠΎΡ€Ρ‹ производства комплСксного туристского ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚Π°, ΠΎΠ±Π»Π°Π΄Π°ΡŽΡ‰Π΅Π³ΠΎ ΡƒΠ½ΠΈΠΊΠ°Π»ΡŒΠ½Ρ‹ΠΌΠΈ цСнностными свойствами

    ΠžΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΠΎΠ½Π½ΠΎ-мСтодичСскиС ΠΈ экономичСскиС основы создания ΡΠ΅Π»ΡŒΡΠΊΠΈΡ… ΠΊΡƒΡ€ΠΎΡ€Ρ‚ΠΎΠ² Π² Π³Π»ΡƒΠ±ΠΈΠ½Π½Ρ‹Ρ… тСрриториях Π³. Π‘ΠΎΡ‡ΠΈ

    Get PDF
    Π“ΠΎΡ€ΠΎΠ΄-ΠΊΡƒΡ€ΠΎΡ€Ρ‚ Π‘ΠΎΡ‡ΠΈ Π²ΠΊΠ»ΡŽΡ‡Π°Π΅Ρ‚ ΠΏΡ€ΠΈΠ±Ρ€Π΅ΠΆΠ½Ρ‹Π΅ ΠΈ Π³ΠΎΡ€Π½Ρ‹Π΅ ΠΊΡƒΡ€ΠΎΡ€Ρ‚Π½ΠΎ-туристскиС кластСры. ΠΠΊΡ‚ΡƒΠ°Π»ΡŒΠ½ΠΎΠΉ ΠΏΡ€ΠΎΠ±Π»Π΅ΠΌΠΎΠΉ Π² соврСмСнных условиях являСтся Π½Π΅ΠΎΠ±Ρ…ΠΎΠ΄ΠΈΠΌΠΎΡΡ‚ΡŒ вовлСчСния Π² ΠΈΠ½Π΄ΡƒΡΡ‚Ρ€ΠΈΡŽ Ρ‚ΡƒΡ€ΠΈΠ·ΠΌΠ° Π³ΠΎΡ€ΠΎΠ΄Π° ΠΏΡ€ΠΈΡ€ΠΎΠ΄Π½ΠΎ-Ρ€Π΅ΠΊΡ€Π΅Π°Ρ†ΠΈΠΎΠ½Π½Ρ‹Ρ… рСсурсов ΠΈ ΠΎΠ±ΡŠΠ΅ΠΊΡ‚ΠΎΠ² историко-ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€Π½ΠΎΠ³ΠΎ наслСдия Π΅Ρ‰Π΅ Π½Π΅ освоСнных Π³Π»ΡƒΠ±ΠΈΠ½Π½Ρ‹Ρ… Π½ΠΈΠ·ΠΊΠΎΠ³ΠΎΡ€Π½Ρ‹Ρ… Ρ‚Π΅Ρ€Ρ€ΠΈΡ‚ΠΎΡ€ΠΈΠΉ, ΠΈΠΌΠ΅ΡŽΡ‰ΠΈΡ… субтропичСскоС ΡΠ΅Π»ΡŒΡΠΊΠΎΡ…ΠΎΠ·ΡΠΉΡΡ‚Π²Π΅Π½Π½ΠΎΠ΅ Π½Π°Π·Π½Π°Ρ‡Π΅Π½ΠΈΠ΅. Π—Π°Π΄Π°Ρ‡Π° исслСдования β€” ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΠΈΡ‚ΡŒ Π½Π°ΡƒΡ‡Π½ΠΎ-мСтодичСскиС, ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΠΎΠ½Π½Ρ‹Π΅ ΠΈ финансовыС прСдпосылки для устойчивого развития этих Ρ‚Π΅Ρ€Ρ€ΠΈΡ‚ΠΎΡ€ΠΈΠΉ. Π’ исслСдовании сформулированы ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΠΎΠ½Π½Ρ‹Π΅, управлСнчСскиС ΠΈ Π·Π°ΠΊΠΎΠ½ΠΎΠ΄Π°Ρ‚Π΅Π»ΡŒΠ½Ρ‹Π΅ аспСкты основного противорСчия, ΠΊΠΎΡ‚ΠΎΡ€ΠΎΠ΅ связано с Ρ€Π΅ΡˆΠ΅Π½ΠΈΠ΅ΠΌ Π΄Π°Π½Π½Ρ‹Ρ… ΠΏΡ€ΠΎΠ±Π»Π΅ΠΌ: нСсоотвСтствиС ΠΏΡ€Π°Π²ΠΎΠ²ΠΎΠ³ΠΎ полоТСния ΠΈ статуса ΡΠ΅Π»ΡŒΡΠΊΠΈΡ… Ρ‚Π΅Ρ€Ρ€ΠΈΡ‚ΠΎΡ€ΠΈΠΉ Ρ€Π΅ΠΊΡ€Π΅Π°Ρ†ΠΈΠΎΠ½Π½Ρ‹ΠΌ Π·Π°Π΄Π°Ρ‡Π°ΠΌ ΠΊΡƒΡ€ΠΎΡ€Ρ‚Π½ΠΎΠΉ мСстности Π³. Π‘ΠΎΡ‡ΠΈ. Для прСодолСния этого противорСчия прСдлагаСтся совмСстноС Ρ€Π°Π·Π²ΠΈΡ‚ΠΈΠ΅ Π°Π³Ρ€Π°Ρ€Π½ΠΎΠΉ Π΄Π΅ΡΡ‚Π΅Π»ΡŒΠ½ΠΎΡΡ‚ΠΈ ΠΈ пСрспСктивных Π²ΠΈΠ΄ΠΎΠ² Ρ‚ΡƒΡ€ΠΈΠ·ΠΌΠ° ΠΈ Ρ€Π΅ΠΊΡ€Π΅Π°Ρ†ΠΈΠΈ Π² Π³Π»ΡƒΠ±ΠΈΠ½Π½Ρ‹Ρ… Π·ΠΎΠ½Π°Ρ… Π³ΠΎΡ€ΠΎΠ΄Π°, Ρ‚Π°ΠΊΠΈΡ… ΠΊΠ°ΠΊ Π»Π΅Ρ‡Π΅Π±Π½ΠΎ-ΠΎΠ·Π΄ΠΎΡ€ΠΎΠ²ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹ΠΉ, сСльский, этничСский ΠΈ Π½Π°ΡƒΡ‡Π½ΠΎ-ΠΏΠΎΠ·Π½Π°Π²Π°Ρ‚Π΅Π»ΡŒΠ½Ρ‹ΠΉ Ρ‚ΡƒΡ€ΠΈΠ·ΠΌ. Π‘ΠΎΡ†ΠΈΠ°Π»ΡŒΠ½ΠΎ-экономичСскоС влияниС развития Ρ‚ΡƒΡ€ΠΈΠ·ΠΌΠ° ΠΈ Ρ€Π΅ΠΊΡ€Π΅Π°Ρ†ΠΈΠΈ Π½Π° сСльской Ρ‚Π΅Ρ€Ρ€ΠΈΡ‚ΠΎΡ€ΠΈΠΈ ΠΊΡƒΡ€ΠΎΡ€Ρ‚Π° обСспСчит рост занятости ΠΈ ΠΏΡ€Π΅Π΄ΠΏΡ€ΠΈΠ½ΠΈΠΌΠ°Ρ‚Π΅Π»ΡŒΡΠΊΠΎΠΉ активности Π² связи с появлСниСм спСциализированной Ρ€Π΅ΠΊΡ€Π΅Π°Ρ†ΠΈΠΎΠ½Π½ΠΎΠΉ инфраструктуры, Π° Ρ‚Π°ΠΊΠΆΠ΅ сдСлаСт Π²ΠΎΠ·ΠΌΠΎΠΆΠ½Ρ‹ΠΌ ΠΏΠΎΠ²Ρ‹ΡˆΠ΅Π½ΠΈΠ΅ уровня ΠΆΠΈΠ·Π½ΠΈ мСстного Π°Π²Ρ‚ΠΎΡ…Ρ‚ΠΎΠ½Π½ΠΎΠ³ΠΎ насСлСния. Π‘ΠΎΠ·Π΄Π°Π½ΠΈΠ΅ ΡΠ΅Π»ΡŒΡΠΊΠΈΡ… ΠΊΡƒΡ€ΠΎΡ€Ρ‚ΠΎΠ² ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΡ‚ ΠΏΠΎΠ»Π½ΠΎΡ†Π΅Π½Π½ΠΎ ΠΈΡΠΏΠΎΠ»ΡŒΠ·ΠΎΠ²Π°Ρ‚ΡŒ Π±Π°Π»ΡŒΠ½Π΅ΠΎΠ»ΠΎΠ³ΠΈΡ‡Π΅ΡΠΊΠΈΠ΅ рСсурсы, ΠΏΡ€ΠΈΡ€ΠΎΠ΄Π½Ρ‹Π΅ Π»Π΅Ρ‡Π΅Π±Π½Ρ‹Π΅ Ρ„Π°ΠΊΡ‚ΠΎΡ€Ρ‹, ΡƒΠ½ΠΈΠΊΠ°Π»ΡŒΠ½ΠΎΠ΅ историко-ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€Π½ΠΎΠ΅ наслСдиС, ΠΏΡ€ΠΈΡ€ΠΎΠ΄Π½Ρ‹Π΅ Π»Π°Π½Π΄ΡˆΠ°Ρ„Ρ‚Ρ‹ ΠΈ возмоТности субтропичСского сСльского хозяйства Π² туристских цСлях. Π Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ‹ исслСдования Π΄Π°ΡŽΡ‚ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡ‚ΡŒ Ρ€Π°ΡΡΠΌΠ°Ρ‚Ρ€ΠΈΠ²Π°Ρ‚ΡŒ пространствСнноС Ρ€Π°Π·Π²ΠΈΡ‚ΠΈΠ΅ Ρ‚ΡƒΡ€ΠΈΠ·ΠΌΠ° Π½Π° ΠΊΡƒΡ€ΠΎΡ€Ρ‚Π΅, Ρ€Π°Π·Ρ€Π°Π±Π°Ρ‚Ρ‹Π²Π°Ρ‚ΡŒ ΠΈ Ρ€Π΅Π°Π»ΠΈΠ·ΠΎΠ²Π°Ρ‚ΡŒ обоснованныС ΠΏΡ€ΠΎΠ³Ρ€Π°ΠΌΠΌΡ‹ создания ΡΠ΅Π»ΡŒΡΠΊΠΈΡ… ΠΊΡƒΡ€ΠΎΡ€Ρ‚ΠΎΠ² ΠΈ туристских агродСстинаций. ΠŸΠΎΡΠ»Π΅Π΄ΡƒΡŽΡ‰ΠΈΠ΅ исслСдования Π±ΡƒΠ΄ΡƒΡ‚ посвящСны ΠΈΠ·ΡƒΡ‡Π΅Π½ΠΈΡŽ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½Ρ‹Ρ… способов трансформации рСсурсов ΡΠ΅Π»ΡŒΡΠΊΠΈΡ… Ρ‚Π΅Ρ€Ρ€ΠΈΡ‚ΠΎΡ€ΠΈΠΉ Π² Ρ„Π°ΠΊΡ‚ΠΎΡ€Ρ‹ производства комплСксного туристского ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚Π°, ΠΎΠ±Π»Π°Π΄Π°ΡŽΡ‰Π΅Π³ΠΎ ΡƒΠ½ΠΈΠΊΠ°Π»ΡŒΠ½Ρ‹ΠΌΠΈ цСнностными свойствами

    Associations between SNPs and vegetation indices: unraveling molecular insights for enhanced cultivation of tea plant (Camellia sinensis (L.) O. Kuntze)

    No full text
    Background Breeding programs for nutrient-efficient tea plant varieties could be advanced by the combination of genotyping and phenotyping technologies. This study was aimed to search functional SNPs in key genes related to the nitrogen-assimilation in the collection of tea plant Camellia sinensis (L.) Kuntze. In addition, the objective of this study was to reveal efficient vegetation indices for phenotyping of nitrogen deficiency response in tea collection. Methods The study was conducted on the tea plant collection of Camellia sinensis (L.) Kuntze of Western Caucasus grown without nitrogen fertilizers. Phenotypic data was collected by measuring the spectral reflectance of leaves in the 350–1100 nm range calculated as vegetation indices by the portable hyperspectral spectrometer Ci710s. Single nucleotide polymorphisms were identified in 30 key genes related to nitrogen assimilation and tea quality. For this, pooled amplicon sequencing, SNPs annotation and effect prediction with SnpEFF tool were used. Further, a linear regression model was applied to reveal associations between the functional SNPs and the efficient vegetation indices. Results PCA and regression analysis revealed significant vegetation indices with high R2 values (more than 0.5) and the most reliable indices to select ND-tolerant genotypes were established: ZMI, CNDVI, RENDVI, VREI1, GM2, GM1, PRI, and Ctr2, VREI3, VREI2. The largest SNPs frequency was observed in several genes, namely F3’5’Hb, UFGTa, UFGTb, 4Cl, and AMT1.2. SNPs in NRT2.4, PIP, AlaDC, DFRa, and GS1.2 were inherent in ND-susceptible genotypes. Additionally, SNPs in AlaAT1, MYB4, and WRKY57, were led to alterations in protein structure and were observed in ND-susceptible tea genotypes. Associations were revealed between flavanol reflectance index (FRI) and SNPs in ASNb and PIP, that change the amino acids. In addition, two SNPs in 4Cl were associated with water band index (WBI). Conclusions The results will be useful to identify tolerant and susceptible tea genotypes under nitrogen deficiency. Revealed missense SNPs and associations with vegetation indices improve our understanding of nitrogen effect on tea quality. The findings in our study would provide new insights into the genetic basis of tea quality variation under the N-deficiency and facilitate the identification of elite genes to enhance tea quality

    Population Analysis of <i>Diospyros lotus</i> in the Northwestern Caucasus Based on Leaf Morphology and Multilocus DNA Markers

    No full text
    Diospyros lotus is the one of the most frost-tolerant species in the Diospyros genera, used as a rootstock for colder regions. Natural populations of D. lotus have a fragmented character of distribution in the Northwestern Caucasus, one of the coldest regions of Diospyros cultivation. To predict the behavior of D. lotus populations in an extreme environment, it is necessary to investigate the intraspecific genetic diversity and phenotypic variability of populations in the colder regions. In this study, we analyzed five geographically distant populations of D. lotus according to 33 morphological leaf traits, and the most informative traits were established, namely, leaf length, leaf width, leaf index (leaf to length ratio) and the length of the fourth veins. Additionally, we evaluated the intraspecific genetic diversity of D. lotus using ISSR and SCoT markers and proposed a new parameter for the evaluation of genetic polymorphism among populations, in order to eliminate the effect of sample number. This new parameter is the relative genetic polymorphism, which is the ratio of polymorphism to the number of samples. Based on morphological and genetic data, the northernmost population from Shkhafit was phenotypically and genetically distant from the other populations. The correspondence between several morphological traits (leaf width, leaf length and first to fifth right vein angles) and several marker bands (SCoT5, SCoT7, SCoT30: 800–1500 bp; ISSR13, ISSR14, ISSR880: 500–1000 bp) were observed for the Shkhafit population. Unique SCoT and ISSR fragments can be used as markers for breeding purposes. The results provide a better understanding of adaptive mechanisms in D. lotus in extreme environments and will be important for the further expansion of the cultivation area for persimmon in colder regions

    Population Analysis of Diospyros lotus in the Northwestern Caucasus Based on Leaf Morphology and Multilocus DNA Markers

    No full text
    Diospyros lotus is the one of the most frost-tolerant species in the Diospyros genera, used as a rootstock for colder regions. Natural populations of D. lotus have a fragmented character of distribution in the Northwestern Caucasus, one of the coldest regions of Diospyros cultivation. To predict the behavior of D. lotus populations in an extreme environment, it is necessary to investigate the intraspecific genetic diversity and phenotypic variability of populations in the colder regions. In this study, we analyzed five geographically distant populations of D. lotus according to 33 morphological leaf traits, and the most informative traits were established, namely, leaf length, leaf width, leaf index (leaf to length ratio) and the length of the fourth veins. Additionally, we evaluated the intraspecific genetic diversity of D. lotus using ISSR and SCoT markers and proposed a new parameter for the evaluation of genetic polymorphism among populations, in order to eliminate the effect of sample number. This new parameter is the relative genetic polymorphism, which is the ratio of polymorphism to the number of samples. Based on morphological and genetic data, the northernmost population from Shkhafit was phenotypically and genetically distant from the other populations. The correspondence between several morphological traits (leaf width, leaf length and first to fifth right vein angles) and several marker bands (SCoT5, SCoT7, SCoT30: 800–1500 bp; ISSR13, ISSR14, ISSR880: 500–1000 bp) were observed for the Shkhafit population. Unique SCoT and ISSR fragments can be used as markers for breeding purposes. The results provide a better understanding of adaptive mechanisms in D. lotus in extreme environments and will be important for the further expansion of the cultivation area for persimmon in colder regions
    corecore