Mammoth calf Lyuba had a hump on the neck consisting of special fat cavities.

<p>Mammoth calf Lyuba had a hump on the neck consisting of special fat cavities.</p

Sample characteristics of frozen mammals.

<p>Sample characteristics of frozen mammals.</p

Gas-liquid chromatogram of fatty acid methyl esters from under skin fat from the belly of mammoth Lyuba.

<p>As noted in the chromatogram, palmitic acid (16∶0) is the main FA component, but the peaks due to the monounsaturated 18∶1<i>n</i>-9<i>E</i> and 18∶1<i>n</i>-9<i>Z</i> FAs can be clearly seen too.</p

Total fatty acids and saturated fatty acids composition of the fat from frozen mammals ^a.

<p>^a Mean ± SD of three independent determinations performed by GLC-MS.</p

Approximate original fatty acid profiles calculated for frozen mammals.

<p>^a Only the major FAs have been considered.</p><p>^b A superscript number indicates the FA percentages found in frozen samples, while all derived percentages appear in italics. In reconstructed figures, C18 PUFA percentages are partially subtracted from PA figures; C20 PUFAs from EA. For both bison, reconstructed OA percentages are partially subtracted from PA.</p><p>^c PUFA ratios and PA percentage in agreement with those of grass-fed elephants <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084480#pone.0084480-Meyer1" target="_blank">[19]</a>.</p><p>^d PUFA ratios and PA percentage as grass-fed Siberian <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084480#pone.0084480-Mordovskaya1" target="_blank">[20]</a> and Galician horses <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084480#pone.0084480-GuilGuerrero1" target="_blank">[21]</a>.</p><p>^e PUFA ratios and PA percentage as grass-fed Bison subcutaneous fat <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084480#pone.0084480-Turner1" target="_blank">[22]</a>.</p

Unsaturated fatty acids composition of the fat from frozen mammals^a.

<p>^a Mean ± SD of three independent determinations performed by GLC-MS.</p

Reference database EMBL (ITSCyp)

Fasta file of the EMBL reference database (ITSCyp

Reference database EMBL (gh)

Fasta file of the EMBL reference database (P6 loop of the trnL

Megafauna sequence data

Fasta file containing the sequence data

Reference database Arctic (gh)

Fasta file of the Arctic reference database (P6 loop of the trnL