Chemical Composition and Antimicrobial Activity of the Levisticum Officinale W.D.J. Koch Essential Oil

The chemical composition of industrially obtained Levisticum officinale W.D.J. Koch (lovage) essential oil of Moldovan origin was analysed by means of chromatographic (GC-MS) and spectral (IR, 1H and 13C NMR) methods. The obtained results show that the main components of L. officinale essential oil are monoterpenic hydrocarbons which make up to 53.50% of the total number of components. L. officinale essential oil is also characterized by a high content of oxygenated monoterpenes (alcohols, cetones and esters), which reaches up to 33.60%. For the first time the presence of 6-butyl-cyclohepta-1,4-diene (0.56%) and 7-formyl-4-methyl-cumarine (0.15%) in lovage essential oil is reported. Antibacterial and antifungal activities of mentioned oil were evaluated in vitro on five strains of microorganisms. It was found that lovage volatile oil (L .officinale) exhibits high antibacterial and antifungal properties in the range of concentrations 0.015-0.030%

Chromatographic Analysis of Silybum Marianum (L.) Gaertn. Fatty Oil

The present paper describes biochemical (fatty oil) composition of Silybummarianum (L.) Gaertn. of Moldovan origin. The oil content of the seeds was approximately 25%. Linoleic acid (C18:2), an essential polyunsaturated fatty acid, is the most abundant (48.88%), followed by monounsaturated oleic acid (C18:1, 31.94%) and saturated palmitic acid (C16:0, 7.61%). The RP-HPLC analysis of tocopherols composition, showed as main components α-tocopherol (23.45 mg/100g) and γ–tocopherol (5.60 mg/100g). Based on the obtained results, it was shown that the extracted oil from milk thistle seeds is rich in essential fatty acids (about 50%) and tocopherols (29.09 mg/100g) and it can be used in food preparation

Synthesis and Evaluation of Antimicrobial Activity of Tetranorlabdane Compounds Bearing 1,3,4-Thiadiazole Units

Synthesis of novel tetranorlabdane compounds bearing 1,3,4-thiadiazole units and intermediary tetranorlabdane compounds with thiosemicarbazone fragment has been reported. The structures of the new synthesized compounds were confirmed using IR and 1H, 13C, and 15N NMR spectroscopy. The in vitro antifungal and antibacterial activities of the mentioned compounds have been evaluated. Results of this study have shown that the 1,3,4-thiadiazole-2-imine has excellent activity against tested strains of fungi and species of bacteria at minimum inhibitory concentration values of 0.125 and 2.5 μg/mL, respectively

Synthesis of New Homodrimane Sesquiterpenoids Containing Diazine, 1,2,4-triazole and Carbazole Rings

The study describes the synthesis of 11-homodrim-6,8-dien-12-oic acid N-substituted amides containing diazine, 1,2,4-triazole and carbazole rings based on commercially available sclareolide. The mentioned compounds were prepared for the first time by interaction of the generated in situ acyl chloride with some heterocyclic amines: 2- and 4-aminopyrimidine, 2-aminopyrazine, 3-amino-1,2,4-triazole and N-aminocarbazole. Their structures were fully elucidated by elemental and spectral analyses (IR, 1H and 13C NMR)

Chemical Composition and Assessment of Antimicrobial Activity of Lavender Essential Oil and Some By-Products

The producers of essential oils from the Republic of Moldova care about the quality of their products and at the same time, try to capitalize on the waste from processing. The purpose of the present study was to analyze the chemical composition of lavender (Lavanda angustifolia L.) essential oil and some by-products derived from its production (residual water, residual herbs), as well as to assess their “in vitro” antimicrobial activity. The gas chromatography-mass spectrometry analysis of essential oils produced by seven industrial manufacturers led to the identification of 41 constituents that meant 96.80–99.79% of the total. The main constituents are monoterpenes (84.08–92.55%), followed by sesquiterpenes (3.30–13.45%), and some aliphatic compounds (1.42–3.90%). The high-performance liquid chromatography analysis allowed the quantification of known triterpenes, ursolic, and oleanolic acids, in freshly dried lavender plants and in the residual by-products after hydrodistillation of the essential oil. The lavender essential oil showed good antibacterial activity against Bacillus subtilis, Pseudomonas fluorescens, Xanthomonas campestris, Erwinia carotovora at 300 μg/mL concentration, and Erwinia amylovora, Candida utilis at 150 μg/mL concentration, respectively. Lavender plant material but also the residual water and ethanolic extracts from the solid waste residue showed high antimicrobial activity against Aspergillus niger, Alternaria alternata, Penicillium chrysogenum, Bacillus sp., and Pseudomonas aeroginosa strains, at 0.75–6.0 μg/mL, 0.08–0.125 μg/mL, and 0.05–4.0 μg/mL, respectively

GC-MS ANALYSIS OF THE ESSENTIAL OIL OF SATUREJA SUBSPICATA BARTL. EX VIS. OF MOLDOVAN ORIGIN

For the first time the results of GC-MS analysis of Satureja subspicata L. oil of Moldovan origin are reported. The chemical profile includes forty-four constituents and consists mostly (97.86%) of phenolic monoterpenes, monoterpene hydrocarbons, bicyclic sesquiterpenes and their oxygenated derivatives. A substantial quantitative and qualitative chemical differentiation of S. subspicata oil of Moldovan origin and reported oil of Croatian origin were found. The essential oil of S. subspicata L. plants cultivated in Republic of Moldova belongs to the carvacrol chemotype

Study of biotransformation compounds in callusar culture of Rhodiola rosea specie

Rhodiola rosea L. is a well known species of plants, which has been used medicinally for decades, but study of its pharmacological effects and the compounds responsible for it use still continues. We present data about induced accumulation of secondary metabolites and as well the results of biotransformation of cinamic alcohol in callus culture of R. rosea of Carpathian origin under the influence of same stress factors. The presence of secondary metabolites was investigated by HPLC-MS analysis. The obtained results can be used for selection of valuable genotypes and their future cultivation in artificial conditions

Chemical Profile, Elemental Composition, and Antimicrobial Activity of Plants of the Teucrium (Lamiaceae) Genus Growing in Moldova

Teucrium L. is a widely distributed genus often used for the treatment of digestive disorders and respiratory problems. The aim of the present study was to determine the chemical composition of essential oils and elemental content of the plant species Teucrium polium, Teucrium hircanicum, Teucrium botrys, Teucrium chamaedrys, Teucrium flavum, Teucrium orientale, and Teucrium scordium of Moldovan origin, as well as to evaluate the antimicrobial activity of their extracts. The composition of essential oils was determined using gas chromatography–mass spectrometry (GC–MS), and neutron activation analysis (NAA) was used to assess the elemental composition of plants. Antimicrobial tests were performed in vitro on the Bacillus subtilis, Pseudomonas fluorescens, Xanthomonas campestris, Erwinia amylovora, Erwinia carotovora, and Candida utilis strains using the double-dilution method. GC–MS allowed the identification of 59 components of the analyzed essential oils, and showed that the analyzed species belong to four different chemotypes. Using NAA, 18 major and minor elements, the contents of which fell within the value ranges reported for other medicinal herbs of this genus, were identified. The hydroalcoholic extracts from Teucrium spp. exhibited in vitro antibacterial and antifungal activity at 0.03–0.06% and 0.015–0.03%, respectively. The extracts from Teucrium spp. exhibited high antibacterial and antifungal activity, enabling their application for medical purposes

Chemical Profile, Elemental Composition, and Antimicrobial Activity of Plants of the <i>Teucrium</i> (Lamiaceae) Genus Growing in Moldova

Teucrium L. is a widely distributed genus often used for the treatment of digestive disorders and respiratory problems. The aim of the present study was to determine the chemical composition of essential oils and elemental content of the plant species Teucrium polium, Teucrium hircanicum, Teucrium botrys, Teucrium chamaedrys, Teucrium flavum, Teucrium orientale, and Teucrium scordium of Moldovan origin, as well as to evaluate the antimicrobial activity of their extracts. The composition of essential oils was determined using gas chromatography–mass spectrometry (GC–MS), and neutron activation analysis (NAA) was used to assess the elemental composition of plants. Antimicrobial tests were performed in vitro on the Bacillus subtilis, Pseudomonas fluorescens, Xanthomonas campestris, Erwinia amylovora, Erwinia carotovora, and Candida utilis strains using the double-dilution method. GC–MS allowed the identification of 59 components of the analyzed essential oils, and showed that the analyzed species belong to four different chemotypes. Using NAA, 18 major and minor elements, the contents of which fell within the value ranges reported for other medicinal herbs of this genus, were identified. The hydroalcoholic extracts from Teucrium spp. exhibited in vitro antibacterial and antifungal activity at 0.03–0.06% and 0.015–0.03%, respectively. The extracts from Teucrium spp. exhibited high antibacterial and antifungal activity, enabling their application for medical purposes