Bacteriophages or “bacteria eaters” alternative to antibiotics, vaccine preparation and treatment of severe non-infectious diseases

Bacteriophages are viruses with a simple structure that attack and kill bacteria, but like other more complex relatives they are perfect parasites that survive by infecting a host, in their case bacteria. Bacteriophages are among the most common and diverse organisms in the biosphere, ubiquitous where there are also bacteria. Without being harmful to macro organisms, bacteriophages can be found in ocean and sea water, soils, plants, animals, people, wastewater and food of animal origin contaminated by bacteria. It is believed that there are over 1.031 bacteriophages on earth. Currently, the expansion and intensification of the use of antibiotics has led to the increase of antibiotic resistance at the global level, and the focus of treatments on intestinal microecology has determined the intensification of research on its role in human health

Seasonal syndromic surveillance by sentinel system of ILI, ARI, SARI and pneumonia in Romania – season 2023/2024

Syndromic surveillance was initially developed for early detection compared to traditional methods of an epidemiological event with an impact on public health, but as it was applied by more countries it was observed that it can provide information on the size, dynamics of the spread with an emphasis on the potential for national, regional and global evolution, the severity and the most affected population groups, but also to the development of a quick, specific response. Globalization, which involves the intensification of the movement of people (including sick or incubating infectious diseases), live animals, or products of animal origin in and from any part of the planet, but also climate change and pollution, amplifies the danger of the spread of communicable infectious diseases from areas so-called “specific” diseases in new areas, unknown to populations and healthcare systems. In this context, the One Health initiative needs efficient surveillance methods to help promote the health of people, animals, the environment, and ultimately the planet Earth. Recently, there have been substantial changes in the surveillance and control of infectious diseases with an impact on public health aimed at assessing the risks of the emergence of infectious agents with epidemic and pandemic potential by identifying and analyzing favorable factors related to the infectious agent (virulence, variability, transmissibility, etc.), the host organism (immunity, physiological factors, vaccination status, associated chronic diseases, nutritional status, living conditions, etc.), demographic factors (birth rate, mortality, population agglomerations), climatic factors and insect populations vectors, rodents and wild animals (including birds) and last but not least domestic animal populations (including birds). Among these methods, syndromic surveillance stands out, which in addition has the quality of using automatic data acquisition and generating statistical alerts, monitors disease indicators in real-time or near real-time to detect disease outbreaks earlier than would be possible with conventional methods traditional public health. In this context, we will present the Romanian experience regarding the syndromic surveillance of ILI, ARI and SARI in Romania through the national sentinel system season 2023/2024

The Effect of Clay Type on the Physicochemical Properties of New Hydrogel Clay Nanocomposites

This study focuses on the investigation of clay type effect on the final properties of semi-interpenetrated Salecan/poly(methacrylic acid)/clay hydrogel nanocomposites. Previous studies have indicated that the presence of clay in polymer composites leads to better swelling capacity and mechanical properties as functions of clay type. On the other hand, Salecan, which is a water soluble extracellular polysaccharide, was proved to assure greater flexibility to hydrogels. These properties recommend clay and Salecan for semi-interpenetrated hydrogels preparation with specific application in biomedicine. The purpose was to determine the most suitable type of clay as well as Salecan influence for developing the desired water retention/delivery ability and mechanically enhanced semi-interpenetrating polymer network (SIPN) nanocomposites. For our investigations, we have chosen commercially available montmorillonite (ClNa) and different commercial organomodified clay (Cl30B, Cl20A and Cl15A). Several analyses results (FTIR, TGA, DMA, XRD, microscopy and swelling studies) demonstrated that not only the presence of Salecan but also the clay type influenced the structure and properties of the final nanocomposites

The role of inflammation in diabetic retinopathy in patients with type II diabetes; potential therapeutic perspectives

Diabetic retinopathy is one of the major causes of visual impairment and blindness in adult population. The pathology is complex, the metabolic changes induced by the hyperglycemic environment leading to neurodegeneration, microvascular damage, with secondary ischemic and inflammatory changes in the retina. This review aims to update the literature data related to the role of inflammation in the onset and progression of diabetic retinopathy. Thus, the molecular and biochemical mechanisms triggered by excess glucose increase the expression of genes involved in inflammatory processes, which leads to the synthesis of inflammatory cytokines such as Il1, Il6, TNF alpha as well as complement activation. Furthermore, recent evidence has demonstrated that both systemic and ocular prolonged inflammation are correlated with the progression of diabetic retinopathy. In conclusion, preventing and/or reducing the progression of diabetic retinopathy requires both rigorous glycemic control in diabetic patients and targeted interference of the specific inflammatory pathways involved

Formation of Amyloid-Like Fibrils by Y-Box Binding Protein 1 (YB-1) Is Mediated by Its Cold Shock Domain and Modulated by Disordered Terminal Domains

YB-1, a multifunctional DNA- and RNA-binding nucleocytoplasmic protein, is involved in the majority of DNA- and mRNA-dependent events in the cell. It consists of three structurally different domains: its central cold shock domain has the structure of a β-barrel, while the flanking domains are predicted to be intrinsically disordered. Recently, we showed that YB-1 is capable of forming elongated fibrils under high ionic strength conditions. Here we report that it is the cold shock domain that is responsible for formation of YB-1 fibrils, while the terminal domains differentially modulate this process depending on salt conditions. We demonstrate that YB-1 fibrils have amyloid-like features, including affinity for specific dyes and a typical X-ray diffraction pattern, and that in contrast to most of amyloids, they disassemble under nearly physiological conditions

Nanocomposites Based Electrosensitive Platforms for Nitrite and Biogenic Amines Determination

Highly electrosensitive platforms have been developed using different nanocomposite materials based on carbon allotropes and different metallic nanoparticles for determination of nitrite and biogenic amines (BAs). The nitrification process occurred in soil represents an important source of pollution. The nitrification consists in biological oxidation of the relatively immobile ammonium (NH4+) to highly mobile nitrate, via nitrite. This process is carried out mainly by the ammonia–oxidizing bacteria (Nitrosomonas sp. and Nitrobacter sp.) present in the soil microbial population [1,2]. The nitrite contamination of ground and surface waters represents the major concern associated with the nitrification process. Additionally, the growing needs for food and environmental safety has led to an increase in research for the detection of biogenic amines (BAs) in recent years. Despite the fact that BAs are increasingly present in food and beverages, causing toxic effects in the body, legislation that limits their presence in food chains needs to be updated, thus requiring sensitive tools for their detection [3,4]. Miniaturized analytical tools have been developed based on nanocomposite materials obtained through combination of different carbon allotrope materials (nanoribbons, nanotubes—single and multiwalled—and nanofibers) with metallic nanoparticles (Ag, Au-Ag, Pt, Cu). Thus, carbon based screen-printed electrodes (SPE) were chemically modified with the obtained nanocomposite materials and further characterized using different electrochemical techniques. In order to allow a selective and sensitive determination of analytes, an electropolymerized film was deposed on the modified sensors. For BAs determination were realized with two configurations of biosensors, a bienzymatic one consisting in immobilization of diamine oxidase (DAO) and horseradish peroxidase (HRP) onto the modified sensors, and, respectively, a mono-enzymatic system based on immobilization of DAO onto the modified sensors. It was taken into account that the charge of carbon-based nanomaterials on the surface of the sensors should not exceed 5%, in order to ensure a low based current. Morpho-structural and electrochemical characterization studies of the modified SPEs have been performed in order to achieve a high sensitivity and selectivity of detection, applying a low overvoltage. The co-polymeric film ensured a better stability of the nanocomposite material layers at the electrode surface and an optimal matrix for enzymes immobilization. Optimization of the nanocomposite-based sensors were performed, and finally detection of biogenic amines was carried out using biosensors based on single-walled carbon nanotubes and Pt nanoparticles, while nitrite determination was performed using multi-walled carbon nanotubes and AgNP modified sensors at applied potentials between −0.45 and +0.6 V vs. Ag/AgCL. The developed sensors and biosensors showed good sensitivities of nitrite and BAs detection. Although the enzyme DAO has a low enough activity to catalyze the oxidation of amine of interest, the detection limits were lowered due to the electrocatalytic activity of nanocomposite materials and the HRP enzyme used

Composite Hydrogels Based on Poly (Methacrilyc Acid) Reinforced with Laponite Inorganic Filler

Polymeric hydrogels are soft materials that can absorb large amounts of water or biological [...