How to improve the sensitivity of coplanar electrodes and micro channel design in electrical impedance flow cytometry: a study

This paper describes a comprehensive analysis of the geometrical parameters in uencing the sensitivity of a coplanar elec- trode layout for electrical impedance ow cytometry. The designs presented in this work have been simulated, fabricated, and tested. 3D nite element method was applied to simulate and improve the sensitivity of the coplanar designs for two spacings between electrodes. The proposed model uses conditional expressions to de ne spatially dependent material properties. The vertical and lateral sensitivities were evaluated for all the designs. The experimental results obtained with polystyrene beads show good agreement with the simulations. Precentering particles with dielectrophoresis allowed to control their position in the microchannel. The optimized designs are envisioned to be used for sizing and characterizing particles from single cells to cell aggregates

Creation of cell aggregates under flow with dielectrophoresis using planar electrodes

International audienc

Towards more sensitive coplanar electrode designs for analysis of single cells and small cell aggregates by Electrical Impedance Spectroscopy

International audienc

How to improve the sensitivity of coplanar electrodes and micro channel design in electrical impedance flow cytometry: a study

This paper describes a comprehensive analysis of the geometrical parameters in uencing the sensitivity of a coplanar elec- trode layout for electrical impedance ow cytometry. The designs presented in this work have been simulated, fabricated, and tested. 3D nite element method was applied to simulate and improve the sensitivity of the coplanar designs for two spacings between electrodes. The proposed model uses conditional expressions to de ne spatially dependent material properties. The vertical and lateral sensitivities were evaluated for all the designs. The experimental results obtained with polystyrene beads show good agreement with the simulations. Precentering particles with dielectrophoresis allowed to control their position in the microchannel. The optimized designs are envisioned to be used for sizing and characterizing particles from single cells to cell aggregates

Dielectrophoresis‐assisted creation of cell aggregates under flow conditions using planar electrodes

We present a microfluidic platform allowing dielectrophoresis-assisted formation of cell aggregates of controlled size and composition under flow conditions. When specific ex- perimental conditions are met, negative dielectrophoresis allows efficient concentration of cells towards electric field minima and subsequent aggregation. This bottom-up assembly strategy offers several advantages with respect to the targeted application: first, dielec- trophoresis offers precise control of spatial cell organization, which can be adjusted by optimizing electrode design. Then, it could contribute to accelerate the establishment of cell-cell interactions by favoring close contact between neighboring cells. The trapping ge- ometry of our chip is composed of eight electrodes arranged in a circle. Several parameters have been tested in simulations to find the best configurations for trapping in flow. Those configurations have been tested experimentally with both polystyrene beads and human embryonic kidney cells. The final design and experimental setup have been optimized to trap cells and release the created aggregates on demand

Creation of cell aggregates under flow with dielectrophoresis using planar electrodes

International audienc

Improvement of the sensitivity of a coplanar electrode layout for Electrical Impedance Spectroscopy

International audienc

A new planar electrode design for dielectrophoresis-assisted creation of cell aggregates under flow conditions

International audienc

Cell trapping and aggregation in flow with dielectrophoresis using planar electrodes

prix “early career researcher award”International audienc

Analysis of independent microarray datasets of renal biopsies identifies a robust transcript signature of acute allograft rejection

Transcriptomics could contribute significantly to the early and specific diagnosis of rejection episodes by defining 'molecular Banff' signatures. Recently, the description of pathogenesis-based transcript sets offered a new opportunity for objective and quantitative diagnosis. Generating high-quality transcript panels is thus critical to define high-performance diagnostic classifier. In this study, a comparative analysis was performed across four different microarray datasets of heterogeneous sample collections from two published clinical datasets and two own datasets including biopsies for clinical indication, and samples from nonhuman primates. We characterized a common transcriptional profile of 70 genes, defined as acute rejection transcript set (ARTS). ARTS expression is significantly up-regulated in all AR samples as compared with stable allografts or healthy kidneys, and strongly correlates with the severity of Banff AR types. Similarly, ARTS were tested as a classifier in a large collection of 143 independent biopsies recently published by the University of Alberta. Results demonstrate that the 'in silico' approach applied in this study is able to identify a robust and reliable molecular signature for AR, supporting a specific and sensitive molecular diagnostic approach for renal transplant monitoring