Recommendations for safety and sustainability measures of the EU FP7 Project UDRIVE

The aim of Task 5.1 is to identify and select, among the outcomes of SP4, the results that are relevant to infer recommendations for measures improving road safety and sustainability. Due to time constraint, the analyses and the recommendations have been done in less time that it was planned at the beginning of the project. The key outcomes of the SP4 work with particular reference to crash risk, unsafe driving, and eco-driving will be studied and organized in terms of relevance to safety and sustainability policies and potential actions towards road users, vehicle and road. Recommendations have been developed to propose actions to stakeholders that can be implemented in the near future to increase safety and sustainability of road transport. This work integrates several reviews of different measures implemented previously in France, Germany, Netherlands and United Kingdom in terms of road safety measures. Then, the recommendations consider possible updates of existing measures and the development of new measures. They will include four kinds of areas: • Recommendations in terms of regulation and enforcement measures; • Recommendations for awareness campaigns and training; • Recommendations for design of road infrastructure; • Recommendations for vehicle safety. Looking at road fatalities statistics, we have identified vulnerable road users as a topic which is important to create recommendations for. We have also identified factors that can have an influence on fatality occurrence like age and infrastructure. A report by the World Health Organization in 2015 (WHO, 2015) identified some area’s wherein there is a need for recommendations to improve road safety. We have selected from the by WHO recommended topics, 3 topics which could be explored by naturalistic studies: seat belt, speed, distraction. Another topic that we are looking into is critical situations. The difficulties with investigating critical situations with road fatalities data bases, is that these databases often do not provide fully detailed information about the dynamic of the accident. Naturalistic studies have the ability to explore incidents more in-depth. Another objective of UDRIVE is to improve sustainability by looking into eco-driving. We will look at recommendations for this topic in this report as well

MOESM3 of Enhancing antibody folding and secretion by tailoring the Saccharomyces cerevisiae endoplasmic reticulum

Additional file 3. Screening results for all the strains with double gene additions grouped by strain background, temperature and induction strength. First, the values for the background strain standards are listed, to which the normalization is done always using the reference from the same condition. [AB] stands for antibody titer and SD for standard deviation and specific product yield is defined as [AB]/OD600. The values are means from three biological and two technical replicates

Allooimmunosensitization in left ventricular assist device recipients and impact on post-transplantation outcome

Background: In recent years mechanical circulatory assist devices became an established option in bridging patients with refractory heart failure to heart transplantation. One of the alleged limitations of mechanical devices is a high degree of antibody production with possible deleterious effect on subsequent heart transplantation outcome. Aim: The main goal of this study is to assess the role of antibodies on the outcome of surgical treatment of patients with end- stage heart failure. Method: Firstly, we present a literature review on the current state of knowledge of possible immunologic mechanisms involved in antibody production in left ventricular assist device (LVAD) recipients, new methods of antibody detection, desensitization strategies and overview of published evidence assessing the impact of sensitization on post-transplantation outcome. In the experimental part of our study we prospectively evaluated the presence of anti-Angiotensin II Type 1 Receptor (AT1R) antibodies in 83 Heart Mate II (HMII) recipients who were implanted at our institution between 2008 and 2012 and survived the first 60 days. On-device survival and device malfunction, major infection, major bleeding and neurologic dysfunction were compared between antibody positive and antibody negative recipients. Out of a total..

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Additional file 5. Result from the frequency analysis showing the strains in the 95th and 5th percentile of individual measurements of specific product yield in each temperature and strain background. To determine which strains were enriched among the highest and lowest values of specific product yield, we conducted a frequency analysis for each strain background, separately for each temperature. Frequency represents the absolute count of measurements from the strain inside the percentile and the relative frequency is the frequency normalized to the total count of measurements in the percentile. P values were calculated with Wilcoxon signed rank test using the respective background strain measurements in the same temperature as the reference

MOESM1 of Enhancing antibody folding and secretion by tailoring the Saccharomyces cerevisiae endoplasmic reticulum

Additional file 1. Results from the screening process for all the strains with single gene additions, divided by the different experimental conditions. Values without defined gene or promoter are the strain background measurements for each conditions. The normalization of samples is always done to the background strain values from the same condition. Specific product yield is defined as antibody titer (denoted [AB]) divided by OD600-value. SD is an abbreviation for standard deviation. The values are means from three biological and two technical replicates