AIRBORNE MYCOBIOTA DETERMINED IN THE NEONATAL UNIT OF THE EGE UNIVERSITY HOSPITAL, BORNOVA, TURKEY

WOS: 000384033100014Pathogenic or opportunist fungi in hospital environments may be a source of infection in susceptible hosts. Especially for newborn intensive care units of hospitals, the control of the fungal spore and knowing the incidence of mycobiota in hospitals are of great importance in order to understand the types of infections and the problems caused by them. In this study, the level of endogenous airborne fungal concentrations and the types of fungi were determined at Ege University Hospital, Faculty of Medicine, Neonatal Unit, which is located centre of Izmir. For this purpose, air samples were collected at 14 selected stations monthly during twelve months with the Merck MAS 100 air sampler. As a result, a total of 2631 fungal colonies were counted. Sixty two fungal species belonging to 12 genera were identified. These species consisted of Aspergillus (12), Penicillium (24), Cladosporium (10), Alternaria (6) and other species (10). The 6 microfungi were identified only in genus level. The most prevalent genera were the common moulds; Aspergillus (39.33 +/- 51.43), Penicillium (23.00 +/- 23.50), Cladosporium (74.33 +/- 63.43) and Alternaria (20.17 +/- 25.09). In this study, aimed to emphasize the importance of monitoring the presence of airborne fungal biota, particularly Aspergillus spp. in the newborn intensive care units where newborns and especially premature babies were hospitalized and opportunistic pathogenic species such as A.flavus, A. fumigatus, A. niger and A. terreus were isolated.Ege University, Science Faculty; BAP Project, Ege UniversityEge University [12 FEN 014]The authors would like to extend their thanks to Ege University, Science Faculty. This work was supported by BAP Project (12 FEN 014), Ege University

ISOLATION AND IDENTIFICATION OF ENTOMOPATHOGENIC FUNGI BEAUVERIA BASSIANA FROM TURKEY

WOS: 000391345600012The control of the agricultural pests is crucial for commercial products. There is a growing interest for biological control of these pests and one of the most effective and safe biocontrol agents is Beauveria bassiana (Balsamo-Crivelli) Vuillemin, which is an entomopathogenic fungus. The soil environment is a significant resource for entomopathogenic fungi. In this study the diversity and distribution of entomopathogenic fungus Beauveria bassiana (Bals.-Criv.) Vuill. was assessed throughout an extensive soil survey in Turkey carried out during 2013 and 2014. Soil samples from the seven regions of Turkey were taken and Beauveria spp. isolates were obtained from six of these regions, with 32 positive sites out of 150 sites sampled. The isolates were identified as Beauveria bassiana based on phenotypic and molecular data. Beauveria bassiana was isolated from the European (Marmara) as well as the Asiatic peninsula (Anatolia) of Turkey

Synthesis, characterization and electrochemical behavior of oxo-bridged (arylimido)[tris(3,5-dimethylpyrazolyl)borato] molybdenum(V) complexes

International audienceReaction of the oxo-molybdenum(V) precursor [MoTp*(O)Cl2] [Tp* = hydrotris(3,5-dimethyl-1-pyrazolyl)borate] with H2NC6H4R-4 (R = OEt; OPr) in refluxing toluene in the presence of Et3N afforded the binuclear oxo-bridged oxo(arylimido) molybdenum(V) complexes [Tp*Mo(O)Cl](μ-O)[Tp*Mo(NC6H4OR-4)Cl]. Surprisingly, a similar reaction between [MoTp*(O)Cl2] and C6H5NH2 yielded the previously reported compound [{MoTp*(O)Cl}2(μ-O)] as the only product. The new compounds were characterized by microanalytical data, mass spectrometry, IR and 1H NMR spectroscopy. Cyclic voltammetric studies of the new compounds, of the previously reported compounds [Tp*Mo(O)Cl](μ-O)[Tp*Mo(NAr)Cl] (Ar = C6H4OMe-4, C6H4F-3, C6H4Cl-4, C6H4Br-4, and C6H4I-3), and of [{MoTp*(O)Cl}2(μ-O)] revealed a reversible one-electron oxidation process that is little affected by the nature of the substituent on the aryl group, whereas it is greatly affected by replacement of the imido ligand with an oxo ligand. The [{MoTp*(O)Cl}2(μ-O)] compound also shows a one-electron reduction process

AIRBORNE MICROFUNGUS FLORA DETERMINED IN THE DIFFERENT UNITS OF THE DEPARTMENT OF TULAY AKTAS ONCOLOGY HOSPITAL, EGE UNIVERSITY

WOS: 000351332500008Recently, there has been a dramatic increase in the prevalence of nosocomial fungal infections.; Especially with the increase in the number of immunosuppressed patients, rare fungal pathogens which were difficult to detect in the past are now frequently isolated. In this study, the level of endogenous airborne fungal concentrations and the types of fungi were determined at Ege University, Faculty of Medicine, Tulay Aktas Oncology Hospital. For this purpose, air samples were collected at 34 different locations monthly during six months with the Merck MAS 100 air sampler. As a result, a total of 5590 fungal colonies in 204 petri dishes were counted. Thirty-two fungal species belonging to 10 genera were identified. The most prevalent genera were the common moulds; Aspergillus (3.91 +/- 1.01), Penicillium (16.08 +/- 3.10), Cladosporium (40.82 +/- 4.15) and Alternaria (7.51 +/- 1.82). The results revealed that the most common genus was Cladosporium. Fungal concentration levels in different parts of the stem cell transplantation department ranged from 0 to 340 cfu/m(3). In other units of the hospital, fungal load was found to be between 0 and 45600 cfu/m3. When the fungal density of the stem cell transplantation department and that of the other units were compared, fungal density of the stem cell transplantation department was found to be significantly lower (p = 0.000 <0.05). The highest values in our study were determined during the 5th month of this study, because natural gas pipelines were installed around the hospital in this month. In this study, we aimed to emphasize the importance of monitoring the presence of airborne fungal flora, particularly Aspergillus spp, in the departments of a hospital where patients of high-risk groups are hospitalized.BAP Project, Ege UniversityEge University [03-FEN-009]The authors would like to extend their thanks to Ege University, Science Faculty. This work was supported by BAP Project (03-FEN-009), Ege University

Indoor airborne fungal pollution in newborn units in Turkey

WOS: 000404652900059PubMed ID: 28667414Pathogenic and/or opportunistic fungal species are major causes of nosocomial infections, especially in controlled environments where immunocompromised patients are hospitalized. Indoor fungal contamination in hospital air is associated with a wide range of adverse health effects. Regular determination of fungal spore counts in controlled hospital environments may help reduce the risk of fungal infections. Because infants have inchoate immune systems, they are given immunocompromised patient status. The aim of the present study was to evaluate culturable airborne fungi in the air of hospital newborn units in the Thrace, Marmara, Aegean, and Central Anatolia regions of Turkey. A total of 108 air samples were collected seasonally from newborn units in July 2012, October 2012, January 2013, and April 2013 by using an air sampler and dichloran 18% glycerol agar (DG18) as isolation media. We obtained 2593 fungal colonies comprising 370 fungal isolates representing 109 species of 28 genera, which were identified through multi-loci gene sequencing. Penicillium, Aspergillus, Cladosporium, Talaromyces, and Alternaria were the most abundant genera identified (35.14, 25.40, 17.57, 2.70, and 6.22% of the total, respectively).The Scientific and Technological Research Council of Turkey-TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [111T925]; Management of CBS-KNAW Fungal Biodiversity Centre (Utrecht-The Netherlands)We are very grateful to the "The Scientific and Technological Research Council of Turkey-TUBITAK" for financially supporting our study, Project Number: 111T925. Also special thanks to the Management of CBS-KNAW Fungal Biodiversity Centre (Utrecht-The Netherlands) and Prof. Dr. Robert A. SAMSON for their support in molecular studies in their laboratory for some problematic fungal species from our project and for their kind permission for some of our project staff for working there for certain periods. We wish to thanks Neonatal Managers of Trakya University Medical Faculty, Istanbul University Istanbul Medical Faculty, Eskisehir Osmangazi University Medical Faculty, Ege University Medical Faculty, and Celal Bayar University Medical Faculty for allow our study in mentioned units