Evidence of abundant stop codon readthrough in Drosophila and other Metazoa

While translational stop codon readthrough is often used by viral genomes, it has been observed for only a handful of eukaryotic genes. We previously used comparative genomics evidence to recognize protein-coding regions in 12 species of Drosophila and showed that for 149 genes, the open reading frame following the stop codon has a protein-coding conservation signature, hinting that stop codon readthrough might be common in Drosophila. We return to this observation armed with deep RNA sequence data from the modENCODE project, an improved higher-resolution comparative genomics metric for detecting protein-coding regions, comparative sequence information from additional species, and directed experimental evidence. We report an expanded set of 283 readthrough candidates, including 16 double-readthrough candidates; these were manually curated to rule out alternatives such as A-to-I editing, alternative splicing, dicistronic translation, and selenocysteine incorporation. We report experimental evidence of translation using GFP tagging and mass spectrometry for several readthrough regions. We find that the set of readthrough candidates differs from other genes in length, composition, conservation, stop codon context, and in some cases, conserved stem–loops, providing clues about readthrough regulation and potential mechanisms. Lastly, we expand our studies beyond Drosophila and find evidence of abundant readthrough in several other insect species and one crustacean, and several readthrough candidates in nematode and human, suggesting that functionally important translational stop codon readthrough is significantly more prevalent in Metazoa than previously recognized.National Institutes of Health (U.S.) (U54 HG00455-01)National Science Foundation (U.S.) (CAREER 0644282)Alfred P. Sloan Foundatio

Molecular Characterization of Haemaphysalis Species and a Molecular Genetic Key for the Identification of Haemaphysalis of North America

Haemaphysalis longicornis (Acari: Ixodidae), the Asian longhorned tick, is native to East Asia, but has become established in Australia and New Zealand, and more recently in the United States. In North America, there are other native Haemaphysalis species that share similar morphological characteristics and can be difficult to identify if the specimen is damaged. The goal of this study was to develop a cost-effective and rapid molecular diagnostic assay to differentiate between exotic and native Haemaphysalis species to aid in ongoing surveillance of H. longicornis within the United States and help prevent misidentification. We demonstrated that restriction fragment length polymorphisms (RFLPs) targeting the 16S ribosomal RNA and the cytochrome c oxidase subunit I (COI) can be used to differentiate H. longicornis from the other Haemaphysalis species found in North America. Furthermore, we show that this RFLP assay can be applied to Haemaphysalis species endemic to other regions of the world for the rapid identification of damaged specimens. The work presented in this study can serve as the foundation for region specific PCR-RFLP keys for Haemaphysalis and other tick species and can be further applied to other morphometrically challenging taxa

Politiques de la colère. L'Amérique cherche un renouveau moral dans le brasier de la violence

Irwin Alec. Politiques de la colère. L'Amérique cherche un renouveau moral dans le brasier de la violence. In: Autres Temps. Cahiers d'éthique sociale et politique. N°72, 2001. pp. 69-77

Situation. Une ville blessée

Irwin Alec. Situation. Une ville blessée. In: Autres Temps. Cahiers d'éthique sociale et politique. N°72, 2001. pp. 102-104

Le chrétien comestible. Nourriture et transformation spirituelle chez Simone Weil

Irwin Alec. Le chrétien comestible. Nourriture et transformation spirituelle chez Simone Weil. In: Autres Temps. Cahiers d'éthique sociale et politique. N°62, 1999. pp. 40-50

Le spectacle de la sainteté. Sainteté, sacrifice et signification chez Simone Weil et dans l'éthique américaine contemporaine

Irwin Alec. Le spectacle de la sainteté. Sainteté, sacrifice et signification chez Simone Weil et dans l'éthique américaine contemporaine. In: Autres Temps. Cahiers d'éthique sociale et politique. N°57, 1998. pp. 67-81

A Conceptual Framework for Action on the Social Determinants of Health

When he announced his intention to create the Commission on Social Determinants of Health (CSDH), WHO Director-General Lee Jong-wook identified the Commission as part of a comprehensive effort to promote greater equity in global health, in a spirit of social justice1. The Commission’s goal, then, is to advance health equity, driving action to reduce health differences among social groups, within and between countries. Getting to grips with this mission requires finding answers to three fundamental problems: 1. Where do health differences among social groups originate, if we trace them back to their deepest roots? 2. What pathways lead from root causes to the stark differences in health status observed at the population level? 3. In light of the answers to the first two questions, where and how should we intervene to reduce health inequities? This paper seeks to make explicit a shared understanding of these issues that can orient the work of the CSDH