ELECTRICAL AND THERMODYNAMIC PROPERTIES OF A COLLAGEN SOLUTION

This paper focuses on measurements of the electrical properties, the specific heat capacity and the thermal conductivity of a collagen solution (7.19% mass fraction of native bovine collagen in water). The results of our experiments show that specific electrical conductivity of collagen solution is strongly dependent on temperature. The transition region of collagen to gelatin has been observed from the measured temperature dependence of specific electrical conductivity, and has been confirmed by specific heat capacity measurements by a differential scanning calorimetry

UNSTEADY FLOW OF THIXOTROPIC COLLAGEN SUBSTANCE IN PIPES

Unsteady flow of thixotropic liquid in pipes is solved by 1D and 2D numerical methods using the same constitutive equation — the only difference is in the radial diffusion of the structural parameter. Comparison shows that the neglected diffusion of structural parameter implicates a much stronger effect of thixotropy. The models are applied for analysis of the observed hysteresis of hydraulic characteristic of collagen

UNSTEADY FLOW OF THIXOTROPIC COLLAGEN SUBSTANCE IN PIPES

Unsteady flow of thixotropic liquid in pipes is solved by 1D and 2D numerical methods using the same constitutive equation — the only difference is in the radial diffusion of the structural parameter. Comparison shows that the neglected diffusion of structural parameter implicates a much stronger effect of thixotropy. The models are applied for analysis of the observed hysteresis of hydraulic characteristic of collagen

Quantitative Risk Assessment of <i>Bacillus cereus</i> Growth during the Warming of Thawed Pasteurized Human Banked Milk Using a Predictive Mathematical Model

Bacillus cereus is relatively resistant to pasteurization. We assessed the risk of B. cereus growth during warming and subsequent storage of pasteurized banked milk (PBM) in the warmed state using a predictive mathematical model. Holder pasteurization followed by storage below −18 °C was used. Temperature maps, water activity values, and B. cereus growth in artificially inoculated PBM were obtained during a simulation of manipulation of PBM after its release from a Human Milk Bank. As a real risk level, we chose a B. cereus concentration of 100 CFU/mL; the risk was assessed for three cases: 1. For an immediate post-pasteurization B. cereus concentration below 1 CFU/mL (level of detection); 2. For a B. cereus concentration of 10 CFU/mL, which is allowed in some countries; 3. For a B. cereus concentration of 50 CFU/mL, which is approved for milk formulas. In the first and second cases, no risk was detected after 1 h of storage in the warmed state, while after 2 h of storage, B. cereus concentrations of 102 CFU/mL were occasionally encountered. In the third case, exceeding the B. cereus concentration of 102 CFU/mL could be regularly expected after 2 h of storage. Based on these results, we recommend that post-pasteurization bacteriological analysis be performed as recommended by the European Milk Bank Association (EMBA) and using warmed PBM within 1 h after warming (no exceptions)

Bacillus cereus as a Major Cause of Discarded Pasteurized Human Banked Milk: A Single Human Milk Bank Experience

A systematic study, performed from 2017&ndash;2020 looked at the rate of positive post-pasteurization B. cereus findings, the quantity of B. cereus in pasteurized banked human milk (PBM), and the rate of B. cereus toxicogenic isolates from PBM. During the study period, 6815.71 L (30,943 tested bottles) of PBM were tested, with an average amount per year of 1703.93 L (7736 tested bottles). The PBM discard rate per year due to bacterial contamination varied between 8.7&ndash;10.0% and contamination with B. cereus was the most frequent reason. The total number of B. cereus positive tests was 2739 and the proportion of its positivity from all positive tests was between 56.7&ndash;66.6%. The prevalence of B. cereus positive tests rose significantly in the summer months. The production of enterotoxin was found in 3 of the 20 tested samples (15.0%). The B. cereus CFU-quantities in the PBM were below 10 CFU/mL in 80% of cases (16 of 20 samples tested). The quantitative data can be used in the risk assessment of cold storage of PBM at temperatures above zero and manipulation of PBM prior to its administration