Razlike u razinama anti-Müllerovog hormona u krvnoj plazmi i reproduktivnih parametara između dvije vrste goveda Bos indicus i Bubalus bubalis

The aim of this study was to evaluate the possible relationships between reproductive parameters and Anti Mullerian Hormone (AMH) levels in two closely related bovine species. AMH levels and the reproductive parameters of 50 water buffaloes (Bubalus bubalis) and 50 Zebu cattle (Bos indicus) of the same age and raised in similar conditions were evaluated. Clinical data were obtained from the farm in specialized designed format, and AMH was measured by ELISA. The results show that Zebu cattle had higher AMH levels than water buffaloes (P<0.0001), while water buffaloes exhibited better reproductive parameters: days open to pregnancy (P<0.0001), parity (P<0.0005), age at first calving (P<0.0001), and intercalving period (P<0.0001). Although both species have different reproductive parameters, no correlations were found between AMH and the reproductive parameters evaluated in either species. Finally, pregnant Zebu cattle and water buffalo showed a tendency to have lower AMH levels than non-pregnant counterparts. The results are paradoxical as the species with lower AMH levels exhibited better reproductive parameters, suggesting that the reproductive parameters within the herd do not depend solely on the ovarian reserve of females.Cilj je ovog rada bio procijeniti je li moguće ustanoviti povezanost između reproduktivnih parametara i razina anti-Müllerovog hormona (AMH) u dvije blisko srodne vrste goveda. Analizirane su razine AMH i reproduktivni parametri 50 bivola (Bubalus bubalis) i 50 zebu goveda (Bos indicus) iste dobi uzgojenih u sličnim uvjetima. Klinički podatci dobiveni su s farme u posebno osmišljenom pokusu. AMH je određivan pomoću ELISA metode. Rezultati pokazuju da su razine AMH više u goveda nego u bivola (P<0,0001). Bivoli su pokazali bolje reproduktivne parametre: servisno razdoblje (P<0,0001), paritet (P<0,0005), dob kod prvog teljenja (P<0,0001) i međutelidbeno razdoblje (P<0,0001). Premda obje vrste imaju različite reproduktivne parametre, nije ustanovljena povezanost između AMH i analiziranih reproduktivnih parametara niti u jedne od istraživanih vrsta. Zaključno, steone ženke goveda i bivola pokazuju tendenciju manjih razina AMH u usporedbi s nesteonim. Rezultati dobiveni u ovoj studiji su paradoksalni, jer vrste s nižim AMH razinama pokazuju bolje reproduktivne parametre. Ovi rezultati ukazuju na to da reproduktivni parametri unutar krda ne ovise samo o rezervi jajnih stanica u ženki

Resveratrol supplementation promotes recovery of lower oxidative metabolism after vitrification and warming of in vitro -produced bovine embryos

Although vitrification is the current method of choice for oocyte and embryo cryopreservation, it may have detrimental effects on reduction-oxidation status and mitochondrial activity. The aim of this study was to evaluate the effect of supplementing in vitro culture (IVC) media and/or vitrification solutions with the antioxidant resveratrol on active mitochondria, mitochondrial superoxide production and lipid peroxidation. Abattoir-derived oocytes were matured and fertilised in vitro using standard procedures. Following IVF (21 h later), zygotes were cultured in IVC medium supplemented with 0 or 0.5M resveratrol. On Day 7, blastocysts were vitrified using the Cryotech Vitrification Kit (Cryo Tech Laboratory) with or without 0.5M resveratrol. After warming, active mitochondria, mitochondrial superoxide production and lipid peroxidation were evaluated using Mito Tracker Green FM, MitoSOX Red and BODIPY 581/591 C 11 staining respectively. The vitrification-warming process significantly increased active mitochondria and mitochondrial superoxide production in bovine embryos (P < 0.05, ANOVA). The addition of 0.5M resveratrol to the IVC medium or vitrification solutions significantly attenuated the increase in active mitochondria (P < 0.05), but not in mitochondrial superoxide production, whereas embryos cultured and vitrified with resveratrol showed the highest values for both parameters (P < 0.05). Regarding lipid peroxidation, no significant differences were detected between treatments. In conclusion, resveratrol supplementation of IVC medium or vitrification solutions contributes to recovery of an embryo's 'quieter' state (i.e. lower oxidative metabolism) after vitrification. However, supplementation of both solutions with resveratrol seemed to have a pro-oxidant effect.Fil: Madrid Gaviria, Stephania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Morado, Sergio Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Lopez Herrera, Albeiro. Universidad Nacional de Colombia. Sede Medellin; ColombiaFil: Betancur, Giovanni Restrepo. Universidad Nacional de Colombia. Sede Medellin; ColombiaFil: Urrego Alvarez, Rodrigo A.. Universidad Ces.; ColombiaFil: Echeverri Zuluaga, Julián. Universidad Nacional de Colombia. Sede Medellin; ColombiaFil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentin