DETECTION OF THE EFFECT OF GFP ON CELLS AND THE INFLUENCE OF GENE ENGINEERING MANIPULATIONS ON CHICKEN EMBRYOS

Information about the methods of detection of the effect of GFP on cells and influence of genetic engineering on the morphometric trials of chick embryos were studied. Green fluorescent protein (GFP) is rapidly becoming one of the most frequently employed molecular reporters. Its use in monitoring gene expression and protein localization has been well documented. For all morphological parameters, the difference was statistically unreliable. On the length of the torso the difference varied from 0,3 to 5,6%, the length of the head from 0,25 to 5,7%, the length of the neck from 0,35 to 5,8%. Lower length values for all parameters were recorded in eggs obtained from a non-transgenic bird. The change in the bird's genotype did not affect the morphological indices of embryos during incubation. From 3 to 7 days of incubation, the length indicators increased by an average of 2.4 times, and the width by 8.1 times. From 7 to 10 days of incubation, the length increased by an average of 30.7%, and the width by 32,4%

Sex-related of some haematological and serum biochemical changes, fed high-protein diet in Japanese quail (Coturnix japonica)

This study aimed to investigate the effect of highly protein diet on some haematological and serum biochemical parameters in female and male Japanese quail (Coturnix japonica) at different age stages. Three different age stages of birds (0-30, 30-44 and >44 days) were included. After measuring the weight of birds, they were slaughtered and a blood samples were taken. Based on the results, there was an increase in the general protein and albumin in females and males at the age 3 months. The globin was higher in males. Reagrding the ratio of phosphorus and calcium, they were higher in females than males and alkaline phosphate and hemoglobin were elevated in males at 3 months of age, and in females was raised at 1st month. An increase from the normal level of total protein, albumin, calcium, hemoglobin and, alkaline phosphatase and a decrease of phosphorous and globin were observed by ageing. Many of the parameters were different significantly between sexes. © 2021 Iranian Society of Ichthyology

Prevalence and surgical treatment of swelling occurring in Syrian Awassi sheep

This study was conducted on 29740 Syrian Awassi Sheep in different ages and of both genders. The percentage of prevalence swellings was 6.63%, that were caseous lymphadenitis, abscesses, hernias, hygromas, granulomas, heamtomas, and cysts with percentage of 4.875, 1.277, 0.305, 0.147, 0.016, 0.006, and 0.003%, respectively. The percentage of swellings according to gender was 9.91% for females and 1.90% for males. The prevalence percentage of caseous lymphadenitis was 7.525 and 1.065%, abscesses 1.824 and 0.491%, hernia 0.393 and 0.180% in female and male, respectively. There were different types of hernias, where sheep to be affected i.e. abdominal hernia (0.221%), umblica (0.030%), congenital cord umbilical (0.047%) and scrotal hernia (0.006%). The percentage of hygroma in males (0.142%) was higher than females (0.155%); however, the granuloma in females (0.022%) was higher than males (0.008%), and heamatoma was equal in both sexes (0.005%). In addition, the cysts in females (0.005%) was higher than males (0%). The result revealed that infections were higher in females than males in any type of swellings. © 2021 Iranian Society of Ichthyology