Inhibition of cyclophosphomide mutagenicity using Glycyrrihza glabra root extract

The aims of the studying is using methanolic - water extract of the Glycyrrihza glabra root for inhibition the mutagenicity of anticancer drug by cytogenetic assays in vivo in three interaction between the drug and extract. The result shows that the extract is bioantimutagenesis. The treatment by extract after drug causes increase mitotic index and decrease chromosome aberration and sperm head abnormalities. The thin layer chromatography of the extract show that is contains different polar compounds

Protective Activity of Glycyrrihzaglabra against Histopathological Changes in White Albino Rats

The study was aimsto evaluation the protective activity of methanol-water extract of Glycyrrihzaglabraroot (GL) against histopathological changes induced by cyclophosphamide in white albino rats. Multiple doses for drug and extract were used to investigate the accumulation effect of drug and extract onliver and kidney. Results show that drug causes different harmful changes in rat organs in all doses concentrations used in study and extract have protective activity to prevent changes in tissue in 1000, 750 and 250 mg \kg , but dose 500 was failure to protect liver and lowest effect on kidney. Conclusion of present study is Glycyrrihzaglabrauseful for protective body organs against side effect of drugs and harmful effect of oxidative stress. Keywords: Glycyrrihzaglabra  ,cyclophosphamide, liver, kidney

Cytotoxic and Genotoxic Effect of Aflatoxin B1

The aims of study is to determine cytogenetic and genotoxic effect of aflatoxin B1 in vivo, cytogenetic tests are used in studying include chromosome malformation, cell proliferation and plast index test in bone marrow cell in Wight albino rats, the results show that toxin causes Varity in chromosome malformation and increased the rate of it, so it increased in cell proliferation and plast index value compare with negative control

In vivo study of antimutagenic and antioxidant activity of Glycyrrihza glabra root extract

The studying was aimed to inhibition the mutagenecity and oxidation of genomic DNA by use methanol-water extract of Glycyrrihza glabra root (GL) against anticancer drug cyclophosphomide in rats by use DNA fragmentation test, the extract characterized by thin layer chromatography TLC, and antioxidant activity by use β- carotene spray, the experiment design to use tow single dose of GL and single dose of the drug in three interaction, extract before, with and after the drug to investigate the mechanism of which extract effect inside cell. Four multiple doses for drug and extract to investigate the accumulation effect in DNA the results show that extract is contain of different polar compound and have antioxidant activity, so the extract is bio-antimutagenesis and poor dismutagenesis activity, the extract can inhibit accumulation effect of mutagenecity and oxidation activity of the drug

Effect of Pollutant Water on Some Organs and Blood Parameters in Rats

Water pollution causes dangerous problems in our life. The present study aims to determination effect of chemical polluted water on blood parameter and histological changes in rat organs using Wight albino rats. For this study Hilla fabric plant waste water is used as drinking water to the rats for 30 days. Results show that polluted water causes increased in W.B.Cs count and histopathological changes in liver and kidne

Polymorphisms of MicroRNA-146a Gene in Behcet’s Disease in Iraqi Patients

The present study was carried out to detect the association of miR-146a haplotypes polymorphisms with Behcet’s Disease in Iraqi patients, PCR-SSCP technique used in present study, blood was used to DNA extraction, the results show that there was strong association between miR-146a and Behçet’s Disease there were two patterns (A and B), polymorphisms show significant differences (p>0.05) between patients and control where haplotype A was appeared in control with (4% ) while in patients (43%) and haplotype B was appeared in control with (96% ) while in patients (57%). The present study concluded that there was association between miR-146a polymorphisms with Behçet’s Disease, our finding need more investigation to use this polymorphism as early indication of Behçet’s Disease incidence

Estimation Micr-RNA146a Gene Polymorphism in Breast Cancer Tissue

MicroRNAs (miRNAs) are short non-coding RNAs consist of 20-24 nucleotides, it play major roles in all biological pathways in mammals and other multicellular organisms. it effected in numerous cancer-relevant processes like proliferation, cell cycle control, programed cell death, differentiation, migration and metabolism. The present study carried out to estimate Micr-RNA146a gene polymorphism in breast cancer tissue, about 30 sample of embedded tissue were collected after diagnostic it, also 30 sample of healthy blood were collected for comparison , micro-RNA gene was detected using PCR-SSCP technique, the results show that there were 4 haplotypes in this gene A,B,C,D . The Variations in these haplotypes appeared in A and D, haplotypes A and was appeared in 75%, and 100% in patients and control respectively, D disappeared in control but it was 100% in patients . The present study concluded that this gene can be conducted as risk factor in breast cancer, but it need more investigations to detect SNPs and sequencing of micro-RNA146a gene