Genetic Study of TORCH Infections in Women with Bad Obstetric History: Multiplex Polymerase Chain Reaction for Detection of Common Pathogens and Agents of Congenital Infections

To revealed the incidence of TORCH infections among pregnancy wastage in women which had bad obstetric history (BOH). METHODS: The study included 132 women with bad obstetric history. Genetic evaluation for TORCH infections was carried out by specific primers designed for that purpose using PCR method. RESULT: Toxoplasma was 36.36%, rubella 20.45%, cytomegalovirus 29.55% and herpes simplex virus 13.64%. Maximum number of cases of abortion 52 (39.39%), preterm labor 29 (21.96%) was associated with toxoplasma infection, early neonatal deaths 19 (14.39%) were maximally associated with toxoplasma and CMV infections. while congenital malformations 14 (10.6%) were evident maximally with toxoplasma infection and intrauterine death 8 (6.06%). CONCLUSIONS: Women with BOH are significantly higher in infection compared with that in control. A previous history of pregnancy wastage, genetic infestation using specific primers for TORCH agent’s detection and the serological reaction for TORCH infections during current pregnancy must be considered while managing BOH cases so as to reduce the adverse fetal outcome

Seroprevalence study of IgG and IgM Antibodies to Toxoplasma, Rubella, Cytomegalovirus, Chlamydia trachomatis and Herpes simplex II in Pregnancy women in Babylon Province

In this work 180 blood samples was collected from pregnant women in Babylon province, Babylon maternity and children hospital from October/2008 to April/2009. It revealed that TORCH infections was; Cytomegalovirus formed (CMV) 57.2% followed by Toxoplasma gondii 55.5% Rubella 53.9%, Herpes simplex II 28.9% and Chlamydia trachomatis 24.4%. Seroprevalence of Toxoplasma, Rubella, CMV, Chlamydia trachomatis and Herpes IgM Antibodies according to various obstetric losses showed that Abortions happened in all causes with high percentage (Over than 30%) except Herpes infections (less than 6%), while congenital anomalies and premature delivery formed high ratio with some different in some cases. Neonatal deaths are very low under 1% except in CMV infections which formed 4.9%. Distribution of age with type of infection according IgM Antibodies to Toxoplasma, Rubella, CMV, Chlamydia trachomatis and Herpes simplex revealed that major age group for infection was between <20 to 40 years which formed more than two third of all infection cases. Residential distribution with type of infection according IgM Antibodies shows that most infection occurred in rural area (over than 50% in all agents) except in Herpes simplex infections which formed 82.7% in urban area. TORCH (Toxoplasma gondii, Rubella, Cytomegalovirus and Herpes simplex) infections with incidence of abortion in pregnant women in this study revealed that First trimester was the highest ratio of infection than other two trimesters