Extraction and Purification of DNA from Escherichia coli by Kit Promega and Electrophoresis

Orphan Extraction DNA from Escherichia coli by kit promega (# A1120) and accentuated this method positive result to procuration across being quantum from DNA, After that to knowledge any DNA we use Electrophoresis By Agarose gel and accentuated result being genomic DNA and compare with DNA Marker (10 kb) and find that genomic DNA heavydid n't migrant distance about well. Keywords: E.coli , Genomic DNA , Kit Prpmega (#A1120) , Agarose gel

Studying and Determination of Escherichia Coli from for Kinds of Food Samples are Fit for Human Consumption

Twenty plant and animal food samples were collected by from different commercial stores. These samples were placed in a sterilezed plastic bags not to be polluted and were moved to the laboratory in special thermal containers to be tested. The solid samples were processed in the Digester to study each sample separately, while the liquid samples were tested immediately after being diluted. After that they were studied with biochemical tests to identify the type of germ that existed. Results showed that, through these twenty samples the positive samples of the Escherichia coli were (13); (65%) while the negative ones were (7);(35%). Keywords: Escherichia coli- Food samples-Biochemical test