In vitro apoptotic and DNA damaging potential of nanobarium oxide

Saud Alarifi,1 Daoud Ali,1 Widad Al-Bishri2 1Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Department of Biochemistry, Faculty of Science-Al Faisaliah Campus, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: Barium oxide nanoparticles (BaONPs) are an important industrial compound and are widely used in polymers and paints. In this study, apoptotic and genotoxic effects of BaONPs in mouse embryonic fibroblast (L929) cells were determined by using single-cell gel test. In vitro cytotoxicity assays were performed to assess BaONPs’ toxicity in L929 cells. Mild cytotoxicity was observed in L929 cells due to BaONPs. BaONPs increased lipid peroxidation, catalase, and superoxide dismutase levels and lowered glutathione levels in L929 cells. This was accompanied by concomitant generation of reactive oxygen species and activation of caspase-3 in BaONPs-treated L929 cells. On the other hand, when we exposed L929 cells to BaONPs for 24 and 48 hours (comet assay), there was a duration- and dose-dependent increase in DNA impairment detected in the single-cell gel test. Thus, BaONPs exhibit genotoxic and apoptotic effects in L929 cells, most likely due to initiation of oxidative damage. Keywords: nanobarium oxide, L929 cells, DNA damage, apoptosi