Inhibition of cyclophosphomide mutagenicity using Glycyrrihza glabra root extract

The aims of the studying is using methanolic - water extract of the Glycyrrihza glabra root for inhibition the mutagenicity of anticancer drug by cytogenetic assays in vivo in three interaction between the drug and extract. The result shows that the extract is bioantimutagenesis. The treatment by extract after drug causes increase mitotic index and decrease chromosome aberration and sperm head abnormalities. The thin layer chromatography of the extract show that is contains different polar compounds

Cytogenetic Effects of Benzene on Human Blood Cells

The study aims to investigate the cytogenetic effect of benzene on human blood cells in vitro using chromosomes abnormalities and mitotic index test. Different concentrations of benzene were added to human blood culture at 24 hour, then cells were arrested at metaphase to detect chromosomes malformations and its proliferation, the result show that benzene causes increased in mitotic index level and different aberrations in chromosomes which increased with benzene concentrations. Keywords: Benzene, Chromosomes abnormalities, Mitotic index

Cytotoxic and Genotoxic Effect of Aflatoxin B1

The aims of study is to determine cytogenetic and genotoxic effect of aflatoxin B1 in vivo, cytogenetic tests are used in studying include chromosome malformation, cell proliferation and plast index test in bone marrow cell in Wight albino rats, the results show that toxin causes Varity in chromosome malformation and increased the rate of it, so it increased in cell proliferation and plast index value compare with negative control

The Prevalence of Microalbuminuria in Type 2 Diabetes Mellitus Patients in Al-Husain Hospital in Karbala Province- Iraq

This study is performed to assessment of the long term glycaemic hemoglobin (HbA1c) to determine the prevalence of microalbuminuria and to find the risk factors for developing microalbuminuria and consequence nephropathy in patients with type 2 diabetes. The prevalence of microalbuminuria in our study is high (59%) and the percent of and the risk factors that accompanied microalbuminuria are high blood pressure, elevated fasting blood glucose and poor glycemic Diabetes mellitus (D.M.) is a group of metabolic diseases characterized by hyperglycemia poor glycemic control also high  (92.3%) with control

Total and differential leukocytes count in type 2 diabetes mellitus patients in Iraq

To find the correlation between total and differential leukocytes count and the presence of microalbuminuria in diabetic patients. The results show significant positive correlation between total leukocytes count and microalbuminuria in males and females also there is significant positive correlation between neutrophils count and microalbuminuria in males and females but there is no correlation with other types of leukocytes

In vivo study of antimutagenic and antioxidant activity of Glycyrrihza glabra root extract

The studying was aimed to inhibition the mutagenecity and oxidation of genomic DNA by use methanol-water extract of Glycyrrihza glabra root (GL) against anticancer drug cyclophosphomide in rats by use DNA fragmentation test, the extract characterized by thin layer chromatography TLC, and antioxidant activity by use β- carotene spray, the experiment design to use tow single dose of GL and single dose of the drug in three interaction, extract before, with and after the drug to investigate the mechanism of which extract effect inside cell. Four multiple doses for drug and extract to investigate the accumulation effect in DNA the results show that extract is contain of different polar compound and have antioxidant activity, so the extract is bio-antimutagenesis and poor dismutagenesis activity, the extract can inhibit accumulation effect of mutagenecity and oxidation activity of the drug

Effect of Pollutant Water on Some Organs and Blood Parameters in Rats

Water pollution causes dangerous problems in our life. The present study aims to determination effect of chemical polluted water on blood parameter and histological changes in rat organs using Wight albino rats. For this study Hilla fabric plant waste water is used as drinking water to the rats for 30 days. Results show that polluted water causes increased in W.B.Cs count and histopathological changes in liver and kidne

Inhibition the mutagenicity of vitamin a by using Olea europaea extract

This study aims to investigate the predisposition of the extraction of the extract of olive leaves (Olea europaea of the family Oleaceae, or the olive family) to frustrate the excretion of the inherent toxic caused by vitamin A (henceforth VA). After being sure that the extraction is inane of any negative effects on the living creature, the following tests are adopted, the mitotic index test of the medulla ossium of the bones, and the chromosomal aberration test. The results showed that toxic dose of vitamin A have cytogenetic effect. It cusses increased in bone marrow cell proliferation and increase in chromosome malformation, the olea leaf extract showed anti-mutagenic activity against the cytogenetic effect of vitamin A it decrees chromosome malformation and kept the Mitotic index in normal level

The effect of Cyclophosphamide on spermatogenesis in rats

This study aimed to evaluate the effects of cyclophosphamide on spermatogenesis; we used three doses with variable time interval to determine the effect of the low and high doses of cyclophosphamide. The results showed that low doses for long time interval caused a considerable increase in the percentage of sperm head abnormalities (Tertatospermia), without any significant changes in tissue sections, the percentage of sperm head abnormalities was increased to 20.72% with the dose 5mg/Kg, while high doses caused a significant tissue changes in testes, and epididymis as well as head sperm abnormalities but less than the 5mg/Kg dose. The percentage of head sperm abnormalities were 14.75 and 13.19 for the doses 15mg/Kg and 10mg/Kg respectively