Comparative Study of Wound Healing After Treatment With Crude Date Extract and Silver Sulphadiazine

Crude date extract in comparison with silver sulphadiazine was tested to evaluate their effects on wound healing and their antimicrobial activities against the growth of Staphylococcus aureus. Crude date extract 5% (w/v) and silver sulphadiazine 20% (w/v) inhibited the growth of Staphylococcus aureus by 67% and 87% respectively. However,10% and 20% (w/v) date extract showed 100% inhibition. Date extract was fractionated using different solvents with different polarities; all fractions were tested for their antimicrobial activities. Ethyl acetate fraction was found to have inhibitory activity against the growth of Staphylococcus aureus. Preparative TLC was done for ethyl acetate fraction which was further identified to be terpenoidal compound. The histological changes in healing wounds have also been investigated after treatment with 20% and 40% crude date extract ,and silver sulphadiazine and amoxicillin by light microscopy.  The results obtained from 20% CDE treatment as compared to untreated control indicated that, this treatment promotes the process of wound healing and stimulates fibroblast, collagen and epithelialization significantly. This effect was comparable with the effect of silver sulphadiazine treatment