Bandwidth Consideration for Cellular System Upgrade in Developing Countries

Copyright © 2023 Copyright held by the owner/author(s). This work looks into employing simulation-based approach to consider bandwidth aspects when designing/upgrading current/future cellular systems in developing countries. Bandwidth is an important resource that needs to be used optimally and plays a significant role in determining the maximum number users a system can accommodate. Bandwidth directly impacts both the capacity that can be supported and limiting end to end delay within acceptable ranges. This paper presents a scheme to maximize the use of bandwidth considering both capacity and delay aspects and helps to identify major parameters that influence system design

A Clb/Cdk1-mediated regulation of Fkh2 synchronizes CLB expression in the budding yeast cell cycle

Precise timing of cell division is achieved by coupling waves of cyclin-dependent kinase (Cdk) activity with a transcriptional oscillator throughout cell cycle progression. Although details of transcription of cyclin genes are known, it is unclear which is the transcriptional cascade that modulates their expression in a timely fashion. Here, we demonstrate that a Clb/Cdk1-mediated regulation of the Fkh2 transcription factor synchronizes the temporal mitotic CLB expression in budding yeast. A simplified kinetic model of the cyclin/Cdk network predicts a linear cascade where a Clb/Cdk1-mediated regulation of an activator molecule drives CLB3 and CLB2 expression. Experimental validation highlights Fkh2 as modulator of CLB3 transcript levels, besides its role in regulating CLB2 expression. A Boolean model based on the minimal number of interactions needed to capture the information flow of the Clb/Cdk1 network supports the role of an activator molecule in the sequential activation, and oscillatory behavior, of mitotic Clb cyclins. This work illustrates how transcription and phosphorylation networks can be coupled by a Clb/Cdk1-mediated regulation that synchronizes them. © 2017, The Author(s)