FOOD SAFETY PRACTICES AMONG FOOD CARTS IN -NORTH LEBANON

Street food carts serve different types of popular and traditional foods; it is a common economic sector worldwide. In North Lebanon, food carts are mobile or centered in specific places serving common and well known RTE meals. In addition, the increasing incidence of foodborne illnesses associated with street foods sheds light on the importance of inspecting the practices of street vendors. Therefore, the aim of this study is to assess the food safety in 30 food carts using an observational checklist and to perform microbiological analysis for the detection of foodborne pathogens namely; yeast, mold, Listeria monocytogenes, Salmonella, Enterohemorrhagic E.coli, B-glucuronidase E.coli, Enterobacteriaceae and Clostridium perfringens in 10 samples of orange juice, 10 samples of cheese caek and 10 samples of meat shawarma; moreover to investigate the prevalence of Methicillin-resistant Staphylococcus aureus in RTE foods and street vendor’s hands. Out of 30 vendors, 100% were males, 80% with an age between 25-44, 80% of them had less than high school level and 66.7% had fixed stalls. When vendors were observed for food safety practices, 70% and 86.7% of them did not store raw materials separately nor in suitable form respectively, 43.5% cleaned their carts monthly, 96% shared utensils between many types of food, 70% did not clean the counter top surface before starting food preparation; moreover 96%, 76 % and 83% of vendors were not wearing net, gloves nor an appropriate uniform, respectively. Results showed that all tested samples were contaminated with at least one of the tested foodborne pathogens, unsatisfactory levels of yeast and mold were recorded in 10 and 9 orange juice samples, respectively. On the other hand, B-glucuronides E.coli exceeded standard limit in two samples of RTE food, one shawarma sample did not meet standard with respect to C. perfringens, Remarkably, unsatisfactory levels of S. aureus were detected in 55 % of RTE samples and in 30 % of vendor’s hands. S. aureus strains were susceptible to at least one of the used antibiotics, accordingly one isolated from vendor’s hand is considered as MRSA. Therefore, the unsatisfactory levels of yeast, mold and S. aureus in tested samples were induced by vendor’s violation of food safety practices. Consequently, the current proposes to improve the legislation needs to provide safe food for the end consumer

Prevalence and mortality rate of healthcare-associated infections among COVID-19 patients: a retrospective cohort community-based approach

BackgroundThe prevalence of HAI among COVID-19 patients ranged between 4.8% and 42.8% with the highest occurrence observed in critically ill patients. The present study aimed to evaluate the clinical features of HAI in severe and critical COVID-19 patients, their microbiological characteristics, and the attributable risk factors.MethodsThis is an analytical observational, retrospective single-center, cohort study that included 723 patients with severe-critical COVID-19 admitted to Saint George Hospital between September 2020 and February 2021. Data collection included demographic variables (sex, age), comorbidities, laboratory findings, HAI types and agents, COVID-19 treatment modalities, hospitalization settings, length of stay, and mortality rate. Data was analyzed using SPSS version 25.ResultsThe prevalence of patients developing HAI was 7.3% (53 of 723). Five types of nosocomial bacterial infections were tracked noting ventilator-associated pneumonia (41.26%), catheter-associated urinary tract infection (28.6%), hospital-acquired pneumonia (17.44%), catheter-related bloodstream infection (6.35%), and bloodstream infection (6.35%). Binary logistic analysis showed that HAI are statistically affected by four factors noting patients' age (p = 0.039), Length of Stay (p < 0.001), BIPAP (p = 0.019), and mechanical ventilation (p < 0.001). The risk of having HAI increases 3.930 times in case of mechanical ventilation, 2.366 times in case of BIPAP, 1.148 times when the LOS increases 1 day, and 1.029 times when the age is higher with 1 year.ConclusionSince the prevalence of HAI is high among severe and critical COVID-19 patients, it is important to prepare a treatment with diagnostic, preventative, and control measures for this infection