8 research outputs found
Antidiabetic Potentials of Essential Oil Extracted from the Leaves of Hoslundia opposita
Comparison of chemical composition, antioxidant and antibacterial activity of Callistemon citrinus skeels
Optimization studies for encapsulation and controlled release of curcumin drug using Zn+2 cross-linked alginate and carboxy methylcellulose blend
Herbal medicine use and linked suspected adverse drug reactions in a prospective cohort of Ugandan inpatients
Chemical composition of 8 <it>eucalyptus</it> species' essential oils and the evaluation of their antibacterial, antifungal and antiviral activities
<p>Abstract</p> <p>Background</p> <p>In 1957, Tunisia introduced 117 species of <it>Eucalyptus</it>; they have been used as fire wood, for the production of mine wood and to fight erosion. Actually, <it>Eucalyptus</it> essential oil is traditionally used to treat respiratory tract disorders such as pharyngitis, bronchitis, and sinusitis. A few investigations were reported on the biological activities of <it>Eucalyptus</it> oils worldwide. In Tunisia, our previous works conducted in 2010 and 2011 had been the first reports to study the antibacterial activities against reference strains. At that time it was not possible to evaluate their antimicrobial activities against clinical bacterial strains and other pathogens such as virus and fungi.</p> <p>Methods</p> <p>The essential oils of eight <it>Eucalyptus</it> species harvested from the Jbel Abderrahman, Korbous (North East Tunisia) and Souinet arboreta (North of Tunisia) were evaluated for their antimicrobial activities by disc diffusion and microbroth dilution methods against seven bacterial isolates: <it>Haemophilus influenzae</it>, <it>Klebsiella pneumoniae</it>, <it>Pseudomonas aeruginosa</it>, <it>Staphylococcus aureus</it>, <it>Streptococcus agalactiae</it>, <it>Streptococcus pneumoniae</it> and <it>Streptococcus pyogenes</it>. In addition, the bactericidal, fungicidal and the antiviral activities of the tested oils were carried out.</p> <p>Results</p> <p>Twenty five components were identified by GC/FID and GC/MS. These components were used to correlate with the biological activities of the tested oils. The chemical principal component analysis identified three groups, each of them constituted a chemotype. According to the values of zone diameter and percentage of the inhibition (zdi, % I, respectively), four groups and subgroups of bacterial strains and three groups of fungal strains were characterized by their sensitivity levels to <it>Eucalyptus</it> oils. The cytotoxic effect and the antiviral activity varied significantly within <it>Eucalyptus</it> species oils.</p> <p>Conclusions</p> <p><it>E. odorata</it> showed the strongest activity against <it>S. aureus</it>, <it>H. influenzae</it>, <it>S. agalactiae</it>, <it>S. pyogenes</it>, <it>S. pneumoniae</it> and against all the tested fungal strains. In addition, <it>E. odorata</it> oil showed the most cytotoxic effect. However, the best antiviral activity appeared with <it>E. bicostata</it>. Virus pretreatment with <it>E. bicostata</it> essential oil showed better antiviral activity (IC<sub>50</sub> = 0.7 mg/ml, SI = 22.8) than cell-pretreatment (IC<sub>50</sub> = 4.8 mg/ml, SI = 3.33). The essential oil of <it>E. astringens</it> showed antiviral activity only when incubated with virus prior to cell infection. This activity was dose-dependent and the antiviral activity diminished with the decreasing essential oil concentration.</p