24 research outputs found
Insegurança alimentar como indicador de iniqüidade: análise de inquérito populacional
Structural variants can be more informative for disease diagnostics, prognostics and translation than current SNP mapping and exon sequencing
Characterization of tin (II) sulphide thin film synthesized by successive chemical solution deposition
A method for rapidly screening functionality of actin mutants and tagged actins
<p>Recombinant production and biochemical analysis of actin mutants has been hampered by the fact that actin has an absolute requirement for the eukaryotic chaperone CCT to reach its native state. We therefore have developed a method to rapidly screen the folding capacity and functionality of actin variants, by combining <it>in vitro</it> expression of labelled actin with analysis on native gels, band shift assays or copolymerization tests. Additionally, we monitor, using immuno-fluorescence, incorporation of actin variants in cytoskeletal structures in transfected cells. We illustrate the method by two examples. In one we show that tagged versions of actin do not always behave native-like and in the other we study some of the molecular defects of three &bgr;-actin mutants that have been associated with diseases