Effects of high intense exercise on Cu/Zn-superoxide dismutase (Cu/Zn-SOD) concentration in blood, and Cu/Zn-SOD mRNA expression in lymphocytes

The purpose of the present study was to investigate the effects of high intense exercise on Cu/Zn-superoxide dismutase (Cu/Zn-SOD) concentration in human plasma and Cu/Zn-SOD mRNA expression in human lymphocytes. A single bout of 80% VOamax exercise induced a significant increase of the Cu/Zn-SOD concentration in plasma (p<0.05), but did not affect the expression of Cu/Zn-SODmRNA in lymphocytes. The expression of Cu/Zn-SODmRNA in lymphocytes was measured at first, third and last day of a training camp for five days. The expression of Cu/Zn-SODmRNA in lymphocytes in last day of the camp was significantly increased, compared with those in both first and third day of the camp (p<0.01, and p<0.05, respectively). The results obtained in this study suggest that the increase in plasma Cu/Zn-SOD concentration after acute bout of exercise may be occurred by mechanisms of release from tissues into bloodstream. The results of this study also suggest that repeating high intense exercise may promote the expression of Cu/Zn-SODmRNA in lymphocytes and Cu/Zn-SOD activity in lymphocytes

Out of Equilibrium Quantum Field Theory --- Perturbation Theory and Generalized Boltzmann Equation

This paper describes perturbative framework, on the basis of the closed-time-path formalism, in terms of quasiparticle picture for studying quasiuniform relativistic quantum field systems near equilibrium and nonequilibrium quasistationary systems. Two calculational schemes are introduced, the one is formulated on the basis of the initial-particle distribution function and the one is formulated on the basis of the ``physical''-particle distribution function. It is shown that both schemes are equivalent and lead to a generalized kinetic or Boltzmann equation. Concrete procedure of computing a generic amplitude is presented.Comment: 34page

Identification and characterization of the RNA helicase activity of Japanese encephalitis virus NS3 protein

AbstractThe NS3 protein of Japanese encephalitis virus (JEV) contains motifs typical of RNA helicase/NTPase but no RNA helicase activity has been reported for this protein. To identify and characterize the RNA helicase activity of JEV NS3, a truncated form of the protein with a His-tag was expressed in Escherichia coli and purified. The purified JEV NS3 protein showed an RNA helicase activity, which was dependent on divalent cations and ATP. An Asp-285-to-Ala substitution in motif II of the JEV NS3 protein abolished the ATPase and RNA helicase activities. These results indicate that the C-terminal 457 residues are sufficient to exhibit the RNA helicase activity of JEV NS3

Long-term monitoring of the short period SU UMa-type dwarf nova, V844 Herculis

We report on time-resolved CCD photometry of four outbursts of a short-period SU UMa-type dwarf nova, V844 Herculis. We successfully determined the mean superhump periods to be 0.05584(64) days, and 0.055883(3) for the 2002 May superoutburst, and the 2006 April-May superoutburst, respectively. During the 2002 October observations, we confirmed that the outburst is a normal outburst, which is the first recorded normal outburst in V844 Her. We also examined superhump period changes during 2002 May and 2006 April-May superoutbursts, both of which showed increasing superhump period over the course of the plateau stage. In order to examine the long-term behavior of V844 Her, we analyzed archival data over the past ten years since the discovery of this binary. Although photometry is not satisfactory in some superoutbursts, we found that V844 Her showed no precursors and rebrightenings. Based on the long-term light curve, we further confirmed V844 Her has shown almost no normal outbursts despite the fact that the supercycle of the system is estimated to be about 300 days. In order to explain the long-term light curves of V844 Her, evaporation in the accretion disk may play a role in the avoidance of several normal outbursts, which does not contradict with the relatively large X-ray luminosity of V844 Her.Comment: 10 pages, 11 figures, accepted for PAS

Digital quantitative analysis of mast cell infiltration in interstitial cystitis

AimsTo evaluate the significance of mast cell infiltration in interstitial cystitis (IC) by comparison with equally inflamed controls using a digital quantification technique. MethodsBladder biopsy specimens from 31 patients with Hunner type IC and 38 patients with non-Hunner type IC were analyzed. Bladder biopsy specimens from 37 patients without IC, including 19 non-specific chronic cystitis (non-IC cystitis) specimens and 18 non-inflamed bladder (normal bladder) specimens, were used as controls. Mast cell tryptase-, CD3-, CD20-, and CD138-immunoreactive cells were quantified using digital image analysis software to evaluate both mast cell and lymphoplasmacytic cell densities. Mast cell and lymphoplasmacytic cell densities were counted independently in the entire lamina propria and detrusor areas and compared among the four groups. ResultsIn the lamina propria, there were no significant differences in mast cell and lymphoplasmacytic cell densities between Hunner type IC and non-IC cystitis or between non-Hunner type IC and normal bladder specimens. In the detrusor, the mast cell densities were not significantly different among the four groups. Mast cell density was correlated with lymphoplasmacytic cell density, but not with clinical parameters. ConclusionsMast cell density is not significantly different between IC specimens and non-IC control specimens with a similar degree of background inflammation. The intensity of mast cell infiltration generally correlated with that of lymphoplasmacytic cells. We conclude that mast cell count is of no value in the differential diagnosis between IC and other etiologies