液肥濃度と無機養分がキクの黄斑発生に及ぼす影響

　Yellow-leaf-spot, a physiological abnormality occurring in leaves of several chrysanthemum (Chrysanthemum ×morifolium) cultivars harvested from September to October, is a very serious problem in Japan, of which causes have not been well established. Water stress, high temperature, high irradiation or nutrient stresses are possible physiological factors which may lead to yellow-leaf-spot. In the present study, effects of nutrient levels and mineral composition on the occurrence of yellow-leaf-spot were investigated. ‘Seikou-no-makoto’ and ‘Seikou-no-masaru’ plants were grown in 5 nutrient solutions (N 0, 60, 120, 180, 240 ppm based on Enshi-shoho). In ‘Seikou-no-masaru’ no yellow-leaf-spot occurred. However, in ‘Seikou-no-makoto’, the nodal position with spotted leaves and rate of yellow-leaf-spot increased as nutrient levels increased. ‘Seikou-no-makoto’ plants were supplied with 6 different nutrient solutions containing 3 times N, P, K, Ca, Mg or Fe in 1/3 concentration of Enshi-shoho solution for 3 or 14 days. The nodal position with spotted leaves and rate of yellow-leaf-spot was not affected by mineral composition. The nodal position with spotted leaves and rate of yellow-leaf-spot increased with increasing days of application. Both cultivare were supplied with 7 different nutrient solutions with lacked N, P, K, Ca, Mg, Fe or only microelement (no mineral) in 1/2 Enshi-shoho solution for 10 days. In ‘Seikou-no-masaru’, no yellow-leaf-spot occurred. It occurred only in ‘Seikou-nomakoto’. yellow-leaf-spot occurred in control, P, K, Ca, Mg, Fe deficiency and no mineral, but only slightly in all cases. These results suggest that the occurrence of yellow-leaf-spot was dependent on genotype, and that excessive or deficiency specific elemental mineral stress had no significant effect.９月から10月収穫の作型のキク（Chrysanthemum×morifolium）の葉身で発生する黄斑は水ストレス，高温，強日射，養分ストレスが発生要因として考えられている．本実験では無機養分の濃度，バランスが黄斑の発生に及ぼす影響を調査した．'精興の誠'を５種類の濃度の液肥（園試処方Ｎ０，60，120，180，240，300 ppm）で栽培した場合，液肥濃度が高くなるにつれ，黄斑が発生する範囲，程度共に増大する傾向が見られた．'精興の誠'と'精興の勝'に園試処方１/３濃度を基準とし，Ｎ，Ｐ，Ｋ，Ca，Mg，Fe それぞれを基準の３倍になるように作成した液肥を３または14日間与えた場合，'精興の勝'では黄斑発生は見られなかった．'精興の誠'では黄斑発生は見られたが，発生範囲，発生度ともに処理の影響は見られなかった．また，それぞれの無機養分を欠如させた液肥を作成し10日間与えた場合も黄斑発生に影響は見られなかった．これらの結果から，黄斑発生には遺伝的要因が関与しており，特定の無機養分の過不足により引き起こされるものではないと考えられた

Effect of Medium and Planting Data on the Occurrence of Leaf-yellow-spot in Chrysanthemum 'Seikounomakoto'

Effects of medium and planting data on the occurrence of leaf-yellow-spot in chrysanthemum 'Seikounomakoto' Dendranthema grandiflora Kitamura were investigated. Long duration was required until occurrence of leaf yellow-spot. Moreover, advance of leaf-yellow-spot was slow. The occurrence of leaf-yellow-spot was remarkably increased by sandy loam soil, but decreased by peatmoss and sand medium. Regardless of medium, pattern of occurrence of leaf-yellow-spot was similar. The occurrence of leaf-yellow-spot was attected by planting date, and was remarkably increased by high temperature and irradiation, but decreased by low temperature affected the occurence of leaf-yellow-spo

SNP HiTLink: a high-throughput linkage analysis system employing dense SNP data

<p>Abstract</p> <p>Background</p> <p>During this recent decade, microarray-based single nucleotide polymorphism (SNP) data are becoming more widely used as markers for linkage analysis in the identification of loci for disease-associated genes. Although microarray-based SNP analyses have markedly reduced genotyping time and cost compared with microsatellite-based analyses, applying these enormous data to linkage analysis programs is a time-consuming step, thus, necessitating a high-throughput platform.</p> <p>Results</p> <p>We have developed SNP HiTLink (SNP High Throughput Linkage analysis system). In this system, SNP chip data of the Affymetrix Mapping 100 k/500 k array set and Genome-Wide Human SNP array 5.0/6.0 can be directly imported and passed to parametric or model-free linkage analysis programs; MLINK, Superlink, Merlin and Allegro. Various marker-selecting functions are implemented to avoid the effect of typing-error data, markers in linkage equilibrium or to select informative data.</p> <p>Conclusion</p> <p>The results using the 100 k SNP dataset were comparable or even superior to those obtained from analyses using microsatellite markers in terms of LOD scores obtained. General personal computers are sufficient to execute the process, as runtime for whole-genome analysis was less than a few hours. This system can be widely applied to linkage analysis using microarray-based SNP data and with which one can expect high-throughput and reliable linkage analysis.</p

36-month clinical outcomes of patients with venous thromboembolism:GARFIELD-VTE

Background: Venous thromboembolism (VTE), encompassing both deep vein thrombosis (DVT) and pulmonary embolism (PE), is a leading cause of morbidity and mortality worldwide. Methods: GARFIELD-VTE is a prospective, non-interventional observational study of real-world treatment practices. We aimed to capture the 36-month clinical outcomes of 10,679 patients with objectively confirmed VTE enrolled between May 2014 and January 2017 from 415 sites in 28 countries. Findings: A total of 6582 (61.6 %) patients had DVT alone, 4097 (38.4 %) had PE ± DVT. At baseline, 98.1 % of patients received anticoagulation (AC) with or without other modalities of therapy. The proportion of patients on AC therapy decreased over time: 87.6 % at 3 months, 73.0 % at 6 months, 54.2 % at 12 months and 42.0 % at 36 months. At 12-months follow-up, the incidences (95 % confidence interval [CI]) of all-cause mortality, recurrent VTE and major bleeding were 6.5 (7.0–8.1), 5.4 (4.9–5.9) and 2.7 (2.4–3.0) per 100 person-years, respectively. At 36-months, these decreased to 4.4 (4.2–4.7), 3.5 (3.2–2.7) and 1.4 (1.3–1.6) per 100 person-years, respectively. Over 36-months, the rate of all-cause mortality and major bleeds were highest in patients treated with parenteral therapy (PAR) versus oral anti-coagulants (OAC) and no OAC, and the rate of recurrent VTE was highest in patients on no OAC versus those on PAR and OAC. The most frequent cause of death after 36-month follow-up was cancer (n = 565, 48.6 %), followed by cardiac (n = 94, 8.1 %), and VTE (n = 38, 3.2 %). Most recurrent VTE events were DVT alone (n = 564, 63.3 %), with the remainder PE, (n = 236, 27.3 %), or PE in combination with DVT (n = 63, 7.3 %). Interpretation: GARFIELD-VTE provides a global perspective of anticoagulation patterns and highlights the accumulation of events within the first 12 months after diagnosis. These findings may help identify treatment gaps for subsequent interventions to improve patient outcomes in this patient population.</p

木材のDimensional Stabilityに関する研究(第11報) : 強制腐朽試験に供した塩化水素 : ホルムアルデヒド処理材の組織内における菌糸の行動

この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました

Determination of anionic surfactants using newly synthesized n-dialkyl-2,2'-thiacyanine dyes

For the spectrophotometric determination of anionic surfactants in aqueous media, cationic dyes with a 2,2'-thiacyanine skeleton were newly synthesized. Carbon numbers of 4 and 6 in the n-dialkyl chain combined at the N position of 2,2'-thiacyanine are new compounds. Fundamental information, such as the (p)K(a) values, was obtained. These two compounds and purchased diethyl- and dipropyl-2,2'-thiacyanine were examined for the spectrophotometric determination of an anionic surfactant, DBS. Of these, 3,3'-dihexyl-2,2'-thiacyanine (DHT) showed the best sensitivity. The calibration graph for DBS with DHT was linear up to 1×10(−5) M, and the detection limit was 4×10(−7) M. By a hydrophobic interaction, even a cationic surfactant, Zephiramine, and a nonionic surfactant, Triton X-100, could react with DHT. The ion-association constants for these surfactants with DHT were obtained.水溶液一相系でイオン会合反応を利用する陰イオン界面活性剤直接定量のための陽イオン性染料を新規に合成した．基本骨格はシアニンとし，二つのNに炭素数の異なるn -アルキル鎖を導入した四級塩染料を合成した．アルキル鎖の炭素数4と6のものは新規合成化合物であり，(p)K(a)を求めた．炭素数2と3の市販品と合成した陽イオン試薬を用い，吸光光度法により陰イオン界面活性剤（DBS）との最適反応条件を検討した．3,3'-ジヘキシル-2,2'-チアシアニン（DHT，C6の試薬）を用いて，10(－6) MオーダーのDBSを定量することができ，検出下限は4×10(－7) Mであった．また，疎水性相互作用により，非イオン性界面活性剤，陽イオン界面活性剤とも反応することが分かり，DHTとのイオン会合定数を求めた

液肥濃度と無機養分がキクの黄斑発生に及ぼす影響

　Yellow-leaf-spot, a physiological abnormality occurring in leaves of several chrysanthemum (Chrysanthemum ×morifolium) cultivars harvested from September to October, is a very serious problem in Japan, of which causes have not been well established. Water stress, high temperature, high irradiation or nutrient stresses are possible physiological factors which may lead to yellow-leaf-spot. In the present study, effects of nutrient levels and mineral composition on the occurrence of yellow-leaf-spot were investigated. ‘Seikou-no-makoto’ and ‘Seikou-no-masaru’ plants were grown in 5 nutrient solutions (N 0, 60, 120, 180, 240 ppm based on Enshi-shoho). In ‘Seikou-no-masaru’ no yellow-leaf-spot occurred. However, in ‘Seikou-no-makoto’, the nodal position with spotted leaves and rate of yellow-leaf-spot increased as nutrient levels increased. ‘Seikou-no-makoto’ plants were supplied with 6 different nutrient solutions containing 3 times N, P, K, Ca, Mg or Fe in 1/3 concentration of Enshi-shoho solution for 3 or 14 days. The nodal position with spotted leaves and rate of yellow-leaf-spot was not affected by mineral composition. The nodal position with spotted leaves and rate of yellow-leaf-spot increased with increasing days of application. Both cultivare were supplied with 7 different nutrient solutions with lacked N, P, K, Ca, Mg, Fe or only microelement (no mineral) in 1/2 Enshi-shoho solution for 10 days. In ‘Seikou-no-masaru’, no yellow-leaf-spot occurred. It occurred only in ‘Seikou-nomakoto’. yellow-leaf-spot occurred in control, P, K, Ca, Mg, Fe deficiency and no mineral, but only slightly in all cases. These results suggest that the occurrence of yellow-leaf-spot was dependent on genotype, and that excessive or deficiency specific elemental mineral stress had no significant effect.９月から10月収穫の作型のキク（Chrysanthemum×morifolium）の葉身で発生する黄斑は水ストレス，高温，強日射，養分ストレスが発生要因として考えられている．本実験では無機養分の濃度，バランスが黄斑の発生に及ぼす影響を調査した．'精興の誠'を５種類の濃度の液肥（園試処方Ｎ０，60，120，180，240，300 ppm）で栽培した場合，液肥濃度が高くなるにつれ，黄斑が発生する範囲，程度共に増大する傾向が見られた．'精興の誠'と'精興の勝'に園試処方１/３濃度を基準とし，Ｎ，Ｐ，Ｋ，Ca，Mg，Fe それぞれを基準の３倍になるように作成した液肥を３または14日間与えた場合，'精興の勝'では黄斑発生は見られなかった．'精興の誠'では黄斑発生は見られたが，発生範囲，発生度ともに処理の影響は見られなかった．また，それぞれの無機養分を欠如させた液肥を作成し10日間与えた場合も黄斑発生に影響は見られなかった．これらの結果から，黄斑発生には遺伝的要因が関与しており，特定の無機養分の過不足により引き起こされるものではないと考えられた