Effect of chromium toxicity on germination and early seedling growth in melon (Cucumis melo L.)

This study was conducted to determine and compare the inhibitory effects of chromium on seed germination and early seedling growth of melon (Cucumis melo L.). Chromium applications were controls; 2.5, 5, 10, 25, 50, 75, 100, 200 and 300 mgl-1 Cr in germination stage, and controls; 2.5, 5, 10, 20, 30, 40, 50, 60 and 70 mgl-1 Cr in early seedling stage. Excess chromium was limited to germination rate, germination index, mean germination time and germination uniformity index values in germination level. Radicle length, radicle fresh and dry weight, hypocotyll length, hypocotyll fresh and dry weight, growth tolerance index and seedling relative growth rate was negatively affected by the increased chromium concentrations at the seedling stage. Response of seedlings to chromium was more than that of seed germination. This event is based on the impermeability of seed coats and selectivity of embryos against chromium

Uncertainty analysis for seismic hazard in Northern and Central Italy

In this study we examine uncertainty and parametric sensitivity of Peak Ground Acceleration (PGA) and 1-Hz Spectral Acceleration (1-Hz SA) in probabilistic seismic hazard maps (10% probability of exceedance in 50 years) of Northern and Central Italy. The uncertainty in hazard is estimated using a Monte Carlo approach to randomly sample a logic tree that has three input-variables branch points representing alternative values for bvalue, maximum magnitude (Mmax) and attenuation relationships. Uncertainty is expressed in terms of 95% confidence band and Coefficient Of Variation (COV). The overall variability of ground motions and their sensitivity to each parameter of the logic tree are investigated. The largest values of the overall 95% confidence band are around 0.15 g for PGA in the Friuli and Northern Apennines regions and around 0.35 g for 1-Hz SA in the Central Apennines. The sensitivity analysis shows that the largest contributor to seismic hazard variability is uncertainty in the choice of ground-motion attenuation relationships, especially in the Friuli Region (∼0.10 g) for PGA and in the Friuli and Central Apennines regions (∼0.15 g) for 1-Hz SA. This is followed by the variability of the b-value: its main contribution is evident in the Friuli and Central Apennines regions for both 1-Hz SA (∼0.15 g) and PGA (∼0.10 g). We observe that the contribution of Mmax to seismic hazard variability is negligible, at least for 10% exceedance in 50-years hazard. The overall COV map for PGA shows that the uncertainty in the hazard is larger in the Friuli and Northern Apennine regions, around 20-30%, than the Central Apennines and Northwestern Italy, around 10-20%. The overall uncertainty is larger for the 1-Hz SA map and reaches 50- 60% in the Central Apennines and Western Alps

Ocular surface epithelia contain ABCG2-dependent side population cells exhibiting features associated with stem cells.

When cell populations are incubated with the DNA-binding dye Hoechst 33342 and subjected to flow cytometry analysis for Hoechst 33342 emissions, active efflux of the dye by the ABCG2/BCRP1 transporter causes certain cells to appear as a segregated cohort, known as a side population (SP). Stem cells from several tissues have been shown to possess the SP phenotype. As the lack of specific surface markers has hindered the isolation and subsequent biochemical characterization of epithelial stem cells this study sought to determine the existence of SP cells and expression of ABCG2 in the epithelia of the ocular surface and evaluate whether such SP cells had features associated with epithelial stem cells. Human and rabbit limbal-corneal and conjunctival epithelial cells were incubated with Hoechst 33342, and analyzed and sorted by flow cytometry. Sorted cells were subjected to several tests to determine whether the isolated SP cells displayed features consistent with the stem cell phenotype. Side populations amounting to \u3c1% of total cells, which were sensitive to the ABCG2-inhibitor fumitremorgin C, were found in the conjunctival and limbal epithelia, but were absent from the stem cell-free corneal epithelium. Immunohistochemistry was used to establish the spatial expression pattern of ABCG2. The antigen was detected in clusters of conjunctival and limbal epithelia basal cells but was not present in the corneal epithelium. SP cells were characterized by extremely low light side scattering and contained a high percentage of cells that: showed slow cycling prior to tissue collection; exhibited an initial delay in proliferation after culturing; and displayed clonogenic capacity and resistance to phorbol-induced differentiation; all features that are consistent with a stem cell phenotype