Supersymmetric Gauge Theories on Noncommutative Superspace

We study four dimensional supersymmetric gauge theory on the noncommutative superspace, recently proposed by Seiberg. We construct the gauge-invariant action of N=1 super Yang-Mills theory with chiral and antichiral superfields, which has N=1/2 supersymmetry on the noncommutative superspace. We also construct the action of N=2 super Yang-Mills theory. It is shown that this theory has only N=1/2 supersymmetry.Comment: 13 pages, LaTeX; v3: eq.(22) and related errors correcte

Deformed Supersymmetry in Non(anti)commutative N=2 Supersymmetric U(1) Gauge Theory

We study N=2 supersymmetric U(1) gauge theory in non(anti)commutative N=2 harmonic superspace with the chirality preserving non-singlet deformation parameter. By solving the Wess-Zumino gauge preserving conditions for the analytic superfield, we construct the deformed N=(1,0) supersymmetry transformation for component fields up to the first order in the deformation parameter.Comment: 12 pages, LaTe

N=2 Supersymmetric U(1) Gauge Theory in Noncommutative Harmonic Superspace

We study N=2 supersymmetric U(1) gauge theory in the noncommutative harmonic superspace with nonanticommutative fermionic coordinates. We examine the gauge transformation which preserves the Wess-Zumino gauge by harmonic expansions of component fields. The gauge transformation is shown to depend on the deformation parameters and the anti-holomorphic scalar field. We compute the action explicitly up to the third order in component fields and discuss the field redefinitions so that the component fields transform canonically.Comment: 42 pages, LaTeX, v2: references added, v3: minor correction

Non(anti)commutative N=(1,1/2) Supersymmetric U(1) Gauge Theory

We study a reduction of deformation parameters in non(anti)commutative N=2 harmonic superspace to those in non(anti)commutative N=1 superspace. By this reduction we obtain the exact gauge and supersymmetry transformations in the Wess-Zumino gauge of non(anti)commutative N=2 supersymmetric U(1) gauge theory defined in the deformed harmonic superspace. We also find that the action with the first order correction in the deformation parameter reduces to the one in the N=1 superspace by some field redefinition. We construct deformed N=(1,1/2) supersymmetry in N=2 supersymmetric U(1) gauge theory in non(anti)commutative N=1 superspace.Comment: 30 pages, LaTeX, V2: a reference adde

Characterization of a Novel Murine Colon Carcinoma Subline with High-Metastatic Activity Established by in Vivo Selection Method

The establishment of cancer cell lines, which have different metastatic abilities compared with the parental cell, is considered as an effective approach to investigate mechanisms of metastasis. A highly metastatic potential mouse colon cancer cell subline, Colon-26MGS, was derived from the parental cell line Colon-26 by in vivo selection using continuous subcutaneous implanting to immunocompetent mice. To clarify the mechanisms involved in the enhancement of metastasis, morphological characteristics, cell proliferation, and gene expression profiles were compared between Colon-26MGS and the parental cell. Colon-26MGS showed over 10 times higher metastatic ability compared with the parental cell, but there were no differences in morphological characteristics and in vitro proliferation rates. In addition, the Colon-26MGS-bearing mice exhibited no marked change of splenocyte population and lung pre-metastatic niche with tumor-free mice, but there were significant differences compared to Colon-26-bearing mice. RNA-seq analyses indicated that immune costimulatory molecules were significantly up-regulated in Colon-26MGS. These results suggest that Colon-26MGS showed not only higher metastatic activity, but also less induction property of host immune response compared to parental Colon-26. Colon-26MGS has proven to be a novel useful tool for studying multiple mechanisms involving metastasis enhancement

Loss of the Homeodomain Transcription Factor Prep1 Perturbs Adult Hematopoiesis in the Bone Marrow

<div><p>Prep1, a TALE-family homeodomain transcription factor, has been demonstrated to play a critical role in embryonic hematopoiesis, as its insufficiency caused late embryonic lethality associated with defective hematopoiesis and angiogenesis. In the present study, we generated hematopoietic- and endothelial cell-specific Prep1-deficient mice and demonstrated that expression of Prep1 in the hematopoietic cell compartment is not essential for either embryonic or adult hematopoiesis, although its absence causes significant hematopoietic abnormalities in the adult bone marrow. Loss of Prep1 promotes cell cycling of hematopoietic stem/progenitor cells (HSPC), leading to the expansion of the HSPC pool. Prep1 deficiency also results in the accumulation of lineage-committed progenitors, increased monocyte/macrophage differentiation and arrested erythroid maturation. Maturation of T cells and B cells is also perturbed in Prep-deficient mice. These findings provide novel insight into the pleiotropic roles of Prep1 in adult hematopoiesis that were unrecognized in previous studies using germline <i>Prep1</i> hypomorphic mice.</p></div

Impact of Prep1 loss on the differentiation of lymphoid-lineage cells.

<p>(<b>A, B</b>) Representative flow cytometric profiles of T cell progenitor populations stained with anti-CD4 and anti-CD8 (<b>A</b>) and the CD4⁻CD8⁻ cell population stained with anti-CD44 and anti-CD25 (<b>B</b>) in the thymus from <i>Tie2-Cre; Prep</i>1^<i>fl/fl</i> and control <i>Prep</i>1^<i>fl/fl</i> mice. Numbers in each quadrant indicate percentage of total cells analyzed. Bar graphs on the right depict absolute numbers of CD4⁻CD8⁻ (DN), CD4⁺CD8⁺ (DP), CD4⁺CD8⁻ (CD4 SP), CD4⁻CD8⁺ (CD8 SP), CD44⁺ CD25^- (DN1), CD44⁺ CD25⁺ (DN2), CD44^- CD25⁺ (DN3), and CD44^- CD25^- (DN4) cell populations in <i>Tie2-Cre; Prep</i>1^<i>fl/fl</i> (solid bars) and control <i>Prep1</i>^<i>flfl</i> littermate (open bars) mice (mean and SD; n = 4). (<b>C, D</b>) Representative flow cytometric profiles of B-lineage cells in the BM (<b>C</b>) and mature B and T cells in the spleen (<b>D</b>). Numbers indicate percentage of gated cells among the total cells analyzed; pro- and pre-B cells (B220^low IgM^-), immature B cells (B220^low IgM⁺), mature B cells (B220⁺ IgM⁺) in <b>C</b>, B cells (B220⁺ CD3^-), T cells (B220^- CD3⁺) and nonT/nonB cells (B220^- CD3^-). Bar graphs on the right depict absolute numbers of the indicated cell populations in the BM of two femurs and the whole spleen in <i>Tie2-Cre; Prep</i>1^<i>fl/fl</i> (solid bars) and control <i>Prep1</i>^<i>flfl</i> littermate (open bars) mice (mean and SD; n = 4).</p