舞踊の映像記録を用いた振付方法の比較研究

関西学院大

舞踊の映像記録を用いた振付方法の比較研究 : フォーサイスから舞踏まで

関西学院大

MDM2 is a novel E3 ligase for HIV-1 Vif

The human immunodeficiency virus type 1 (HIV-1) Vif plays a crucial role in the viral life cycle by antagonizing a host restriction factor APOBEC3G (A3G). Vif interacts with A3G and induces its polyubiquitination and subsequent degradation via the formation of active ubiquitin ligase (E3) complex with Cullin5-ElonginB/C. Although Vif itself is also ubiquitinated and degraded rapidly in infected cells, precise roles and mechanisms of Vif ubiquitination are largely unknown. Here we report that MDM2, known as an E3 ligase for p53, is a novel E3 ligase for Vif and induces polyubiquitination and degradation of Vif. We also show the mechanisms by which MDM2 only targets Vif, but not A3G that binds to Vif. MDM2 reduces cellular Vif levels and reversely increases A3G levels, because the interaction between MDM2 and Vif precludes A3G from binding to Vif. Furthermore, we demonstrate that MDM2 negatively regulates HIV-1 replication in non-permissive target cells through Vif degradation. These data suggest that MDM2 is a regulator of HIV-1 replication and might be a novel therapeutic target for anti-HIV-1 drug

Simulation of livestock biomass resource recycling and energy utilization model based on dry type methane fermentation system

International Conference of Biomass and Bioenergy 19-20 August 2019, West Java, IndonesiaThis study was aimed at investigating the local livestock biomass volume for dairy farms in Town A, located in eastern Hokkaido, Japan, and at presenting a model of a biogas plant that enables maximizing the use of the available livestock biomass. Using a dairy farm with 250 animals in Town A as a model for a biogas plant based on dry-type methane fermentation system (dry-type biogas plant), we set the operational conditions to an average hydraulic retention time of 20 days, digestion temperature of 55°C, and methane gas yield of 0.12 Nm3 CH4/kg VSA. We compared the biogas production of our presented model with that of a wet-type biogas plant with the same number of animals. The results showed that the dry-type biogas plant produced biogas at 859 Nm3/day, while the wet-type biogas plant produced biogas at 666 Nm3/day. These results indicate that introducing dry-type biogas plants in all dairy farms in Town A would potentially enable semi-solid livestock manure to be processed, which is not amenable to ordinary composting, in addition to the conventional processes being carried out through biogas plants, as well as lead to an increase in the amount of biogas production