MYCL promotes iPSC-like colony formation via MYC Box 0 and 2 domains

iPS細胞作製過程における初期化因子MYCLのタンパク質ドメインの機能解析. 京都大学プレスリリース. 2021-12-20.Protein domain structures affect the quality of stem cells. 京都大学プレスリリース. 2021-12-20.Human induced pluripotent stem cells (hiPSCs) can differentiate into cells of the three germ layers and are promising cell sources for regenerative medicine therapies. However, current protocols generate hiPSCs with low efficiency, and the generated iPSCs have variable differentiation capacity among different clones. Our previous study reported that MYC proteins (c-MYC and MYCL) are essential for reprogramming and germline transmission but that MYCL can generate hiPSC colonies more efficiently than c-MYC. The molecular underpinnings for the different reprogramming efficiencies between c-MYC and MYCL, however, are unknown. In this study, we found that MYC Box 0 (MB0) and MB2, two functional domains conserved in the MYC protein family, contribute to the phenotypic differences and promote hiPSC generation in MYCL-induced reprogramming. Proteome analyses suggested that in MYCL-induced reprogramming, cell adhesion-related cytoskeletal proteins are regulated by the MB0 domain, while the MB2 domain regulates RNA processes. These findings provide a molecular explanation for why MYCL has higher reprogramming efficiency than c-MYC

MYCLはMYC Box 0及び2ドメインを介してiPS細胞様コロニーの形成を促進する

京都大学新制・課程博士博士(医科学)甲第23817号医科博第138号新制||医科||9(附属図書館)京都大学大学院医学研究科医科学専攻(主査)教授 川口 義弥, 教授 藤田 恭之, 教授 江藤 浩之学位規則第4条第1項該当Doctor of Medical ScienceKyoto UniversityDFA