Isolation, identification and characterization of new luminous bacteria from Chah Bahar Port, southern marine habitat of Iran

Coastal region of Chah Bahar port, Sea of Oman, was screened for the presence of bioluminescence bacteria for the first time. Water samples were taken from surface and subsurface layers and immediately spread on nutrient seawater complete (SWC) agar. Luminous colonies were observed after an overnight incubation at 25°C. Among twenty luminous isolates, four of them were selected for preliminary bacterial identification based on morphological and physiological characteristics. 16S rRNA genes of selected bacteria were then sequenced in order to be submitted in GenBank database as new strains and performing phylogenetic analysis. Four different submitted bacterial strains are as follow, Vibrio sp. Persian 1, Vibrio sp. Persian 2, Vibrio sp. Persian 3, and Vibrio sp. Persian 4 with accession numbers of KC505639, KC765088, KC765089, and KC896417, respectively. Light emission of isolated luminous bacteria was measured using luminometer. Vibrio sp. Persian 1 was found as the best light emitter with counts per second/OD 600 nm equal to 10 × 10^6 RLU/Sec/OD. Isolated Vibrio species were tested for their ability to form biofilm. Vibrio sp. Persian 3 showed weak ability to produce biofilm while other species were considered as moderate biofilm producers

Isolation and identification of halophilic bacteria from Urmia Lake in Iran

Halophiles are in all three domains of life: Archaea, Bacteria and Eucarya. Halophilic microorganisms in addition to forming a major part of life biodiversity can have many biotechnological applications. The objective of this research is isolation and identification of halophilic bacteria from Urmia Lake in Iran and the study of its bacterial biodiversity. After sampling of brines from Urmia Lake from 10 stations and depth of approximately 30-50 cm, in April 2011 and transfer to the laboratory in the sterile conditions, samples were enriched and cultured on defined media, and incubated. After appearance of colonies, selected strains were studied based on morphology, physiology and biochemical characteristics. For phylogenetic identification, their genomic DNA were extracted and amplified by PCR technique. Therefore their sequences were determined by genetic experiment based on 16S rRNA gene sequence and their similarity were analysed in GenBank of EzTaxon database. Finally the phylogenetic tree was constructed. Studied strains belonged to three genera: Halomonas 50% (including H. andesensis LC6(T) [12.5%], H. gomseomensis M12(T) [12.5%], H. hydrothermalis Slthf2(T) [12.5%], H. boliviensis LC1(T) [6.25%] and H. janggokensis M24(T) [6.25%]), Salinivibrio 25% (including S. costicola subsp. alcaliphilus DSM 16359(T) [18.75%] and S. sharmensis BAG(T) [6.25%]) and Idiomarina 25% (including I. loihiensis L2TR(T) [25%])

Hemolytic Uremic Syndrome; Report of a Case With late Recovery Of Renal Function

Background and ObjectiveHemolytic uremic syndrome (HUS) is characterized by triad of microangiopathic hemolytic anemia, thrombocytopenia, and acute renal failure. It is more common in children younger than the age of 4 years and is the most common cause of acute renal failure in many parts of the world in this range of age. The classic form of the disease occurs after an episode of acute diarrhea which may lead to chronic renal failure in 9% of cases. Here in we report a case of HUS with recovery of renal function after 15 months of dialysis. Case report A 12 year old boy was admitted with major clinical symptoms including acute bloody diarrhea, followed by acute renal failure, thrombocytopenia and severe microangiopathic hemolytic anemia. Peripheral blood smear showed probability of HUS. Peritoneal dialysis was started and later followed by hemodialysis. Eventually after 15 months of dialysis he obtained normal renal function and now after 3 years he is in good health with normal renal function.Conclusion: Recovery of renal function in HUS is possible even after a prolonged period of renal failure.Keywords: Hemolytic-uremic syndrome, Children, Child, Late recovery

Relationship between antimicrobial therapy and scar formation in urinary tract infection in children

Background: Urinary infection is known as one of the most common bacterial infection in children. Punctual diagnosis and treatment along with the diagnosis of risk factors and long-term follow-up of the patients suffering from urinary infection are the most important factors in order to prevent scar formation in kidneys. This study was carried out in order to analyze time difference among symptoms onset and the start of urinary infection treatment and it has relation with scar formation in kidneys. Methods: This study was done by (Historical cohort ) method on 120 patients they were divided into 2 groups of 60. The treatment was started on time for the first group but for the second with delay. All of the patient suffered from urinary infection and they were DMSA scaned after six months. The scar formation amount in kidneys was compared in two groups and the results were analyzed by SPSS software. Finding: The result showed that 75% of the girls and 25% of the boys out of 120 infected children, suffered from urinary infection. No obvious relation between the scar formation appearance, parents social level and children genders was found. A meaningful relation was found between treatment delay and scar formation in kidney due to urinary infection (RR= 3, CI = 1.4–6.5). The Minimum And Maximum delay time in this treatment were 1 and 21 days respectively. The average of delay time between symptoms onset and treatment start has been 4.8 days. Based on this research, the delay time in treatment more than 6 days may cause kidney scar formation twice as much (RR=2.15, CI=1.1- 4.6). Conclusion: Scar formation risk increase with delay in antimicrobial therapy in UTI. It is strongly recommended to diagnose and begin the treatment as soon as possible to prevent kidney damage properly

Pseudotumor Cerebri after Kidney Transplantation

AbstractBackground and Objectives: Pseudotumor cerebri is defined by the increase of intracranial pressure. It has different atiologies but, many of its causes are idiopathic and typically present on young obese females. Cerebrospinal fluid (CSF) analysis was normal in this case study and there was no evidence of intracranial mass, venous sinus thrombosis, or obstruction in CSF stream.In this study, we have reported a case of Pseudotumor cerebri presented 7 years after a successful kidney transplant, under treatment by Cyclosporine, Methylprednisolon and Azathioprine(AZT).Case Report: The patient was a 17-year old obese female with a body mass index of 30kg/m2 having Pseudotumor cerebri 7 years after a successful kidney transplant. Brain imaging like CT scan & MRA (Magnetic Resonance Angiography) were normal. CSF analysis was normal, but the increase in CSF pressure had been detected. Repetitive lumber punctures was performed with simultaneous Acetazolamid administration. But her headaches were treated even after the continuation of Cyclosporine and Methylprednisolon, anemia, and renal failure. For patients with kidney transplant and headaches, it is necessary to rule out Pseudotumor cerebri as a differential diagnosis. Neurotoxicity of Cyclosporine is not rare and we have to pay close attention to neurologic side effect of this drug as well. After diagnosing Pseudotumor cerebri in such patients, it is necessary to limit the progression of symptoms and avoid the decrease in patient 's visual acuity.Keywords: Pseudotumor Cerebri; Neurotoxicity; Cyclosporin

Isolation and Characterization of Crude Oil Degrading Bacillus Spp.

Today, application of microorganisms for removing crude oil pollution from contaminated sites as bioremediation studies, was considered by scientists because other methods such as surfactant washing and incineration lead to production of more toxic compounds and they are non-economic. Fifteen crude oil degrading Bacillus   spp. were isolated from contaminated sites. Two isolated showed best growth in liquid media with 1-3% (v/v) crude oil and mineral salt medium, then studied for enzymatic activities on tested media. The results showed maximal increase in optical densities and total viable count concomitant with decrease in pH on fifth day of experimental period for bacillus S6. Typical generation time on mineral salt with 1% crude oil is varying between 18-20h, 25-26h respectively for bacillus S6 and S35. Total protein was monitored at determined time intervals as biodegradation indices. Increasing of protein concentration during the incubation period reveals that isolated bacillus can degrade crude oil and increase microbial biomass. These bacillus spp. reduced surface tension from 60 (mN/m) to 31 and 38 (mN/m), It means that these bacillus spp. can produce sufficient surfactant and have good potential of emulsification capacity. The results demonstrated that these bacillus spp. can utilize crude oil as a carbon and energy source