Chemical Decellularization Methods and Its Effects on Extracellular Matrix

Background:  Extracellular matrix (ECM) produced by tissue decellularization processes as a biological scaffold due to its unique properties compared to other scaffolds for migration and implantation of stem cells have been used successfully in the field of tissue engineering and regenerative medicine in the last years. The objective of this manuscript was to provide an overview of the chemical decellularization methods, evaluation of decellularized ECM and the potential effect of the chemical decellularization agents on the biochemical composition.Methods: We searched in Google Scholar, PubMed, Scopus, and Science Direct. The literature search was done by using the following keywords: “ECM, biologic scaffold, decellularization, chemical methods, tissue engineering.” We selected articles have been published from 2000 to 2016, and 15 full texts and 97 abstracts were reviewed.Results:Employing an optimization method to minimize damage to the ECM ultrastructure as for a result of the lack of reduction in mechanical properties and also the preservation of essential proteins such as laminin, fibronectin, Glycosaminoglycans (GAGs), growth factor is required. Various methods include chemical, physical and enzymatic technics were studied. However, on each of these methods can have undesirable effects on ECM.Conclusion: It is suggested that instead of the Sodium dodecyl sulfate (SDS) which have high strength degradation, we can use zwitterionic separately or in combination with SDS. Tributyl phosphate (TBP) due to its unique properties can be used in decellularization process

chemical methods in decellularization process and its effects on extracellular matrix (ECM): narrative review

Objective :Extracellular matrix (ECM)produced by tissue decellularization processes As a biological scaffold Due to its unique properties compared to other scaffolds for migration and implantation of stem cells have been used successfully in the field of tissue engineering and regenerative medicine at the last years. Methods: for this review we searched in google scholar, medline, scopus, sciencedirect .we used extracellular matrix(ECM),biologic scaffold ,decellularization ,chemical methods, tissue engineering as keywords. We selected articles have been published from almost 2000 to 2016Results: 15 full texts and 97 abstract was reviewed. To obtain a intact ECM with preservation of ECM bioactivity. Employing an optimization method to minimize damage to the ECM Ultrastructure As a result of the lack of reduction in mechanical properties and also preservation of important proteins such as laminin, fibronectin, GAGs, growth factor is required. Various methods, including methods of chemical, physical and enzymatic studied and tested. But on each of these methods can have undesirable effects on ECM .Theobjective of this manuscript is to provide an overview of the chemical decellularization methods , Evaluation of decellularizad ECM and the potential affect of the chemical decellularization agents on the biochemical composition, ultrastructure, and mechanical behavior of the ECM scaffold materials.production an intact ECM with preservation of biochemical composition and mechanical behavior requires a correct choice regardingagents and techniques used in decellularization process.Conclusion: It is suggested that insted of the SDS which have high strength degradation we can use zwitterionic separately or in combination with SDS.TBP due to its unique properties can be used in decellularization process

Efficacy of low-level laser therapy on orofacial pain: A literature review

Low-level laser therapy (LLLT) is a therapeutic approach to treat orofacial pain using a 600–1000 nm laser with a <500 mW power. The efficacy of LLLT is due to the chemical reactions causing an anti-inflammatory and analgesic effect on the affected regions. The aim of this study is to review the effects of low-level laser application on orofacial pain in the English articles released since 2017. Our search keywords were 'low-level laser therapy, temporomandibular disease (TMD), mucositis and orofacial pain'. The most relevant papers were clinical trial, review and meta-analysis articles. 26 out of 243 searched articles were selected from PubMed, Google Scholar and Science Direct and reviewed. Most of the studies reported the positive role of LLLT on orofacial pain relief regardless of their variable procedures; however, the exact mechanism of action still remains unclear. Some studies indicated that LLLT has significantly reduced pain, reduceamount of clicking and both masseter and temporal muscles activityin TMD. As an overall result, it is concluded that LLLT can be served as a therapeutic method for myofascial pain, mucositis and temporomandibular joint disorders and this is due to its analgesic features