Soğan sak nematodu (ditylenchus dipsaci)’nun sarımsak bitkisinde moleküler karakterizasyonu

TEZ 635.25/AKAsKaynakça: 61-65 ss.[Özet Yok

Molecular characterization of Ditylenchus dipsaci on Onion in Turkey

WOS:000428804400013Ditylenchus dipsaci is a species complex including diploid and polyploid individuals. The onion race of D. dipsaci is a sensu stricto group and has a wide range of host spectrum. Identification of the D. dipsaci onion race is difficult using morphological and morphometrical methods. Species specific primers are mostly used in molecular approaches for identification of D. dipsaci populations. Fifty one morphologically selected Ditylenchus spp. populations from onion production areas in Turkey were subjected to molecular identification using four D. dipsaci species specific primer sets (PF1-PR1, PF2-PR2, DdpS1-rDNA2, DitNF1- rDNA2, H05-H06) targeting 5.8S and 18S rDNA, ITS1 and flanking ITS regions. Thirty nine percent of the nematode samples were positive with four primers tested, while four of the nematode samples gave specific bands with H05-H06 primers. Ditylenchus dipsaci sensu stricto was identified with specific primer sets in Adana, Hatay, Tekirdag, Bursa, Aksaray, Karaman, Eskisehir and Ankara provinces in Mediterranean, Trace, Aegean and Central Regions in Turkey.Turkish Scientific and Technical Research Council (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [215O468]This research has been financially supported by Turkish Scientific and Technical Research Council (TUBITAK) (Project No: 215O468)

Molecular characterization of Ditylenchus dipsaci on garlic in Turkey

WOS:000492175100001Garlic plant, which has an important place in the world economy as well as in human nutrition, is grown extensively in Turkey. The stem and bulb nematode, Ditylenchus dipsaci (Kuhn) is one of the most important biotic stresses and is under quarantine as it significantly interrupts garlic production wherever it emerges. Thus, being up to date regarding the distribution and population of the stem and bulb nematode, which is locally found in garlic cultivation areas, identification of the races found on the garlic plant at the molecular level and knowing the host spectrum of the races found at the region are of great importance. The objective of this study is to perform species diagnosis using the species-specific molecular markers at ITS-rDNA regions of the stem and bulb nematode isolates collected from the garlic cultivation areas in Turkey in 2016 and 2017. In the study, molecular screening data of the nematodes isolated from the plant and soil samples were analyzed phylogenetically. In this study, upon molecular screening using 9 different species-specific SSR and SCAR primers, it was found that 34 nematode samples of 53 are D. dipsaci. Nematode samples were from the garlic fields in Kastamonu, Amasya, Aksaray, Tekirdag, Tokat, Balikesir, Bursa, Hatay, Gaziantep, Kahramanmaras, Adiyaman, and Kirklareli provinces. Identification of stem and bulb nematodes found in the areas of garlic cultivation with this study will be useful for conscious and efficient control applications to this nematode.Turkish Scientific and Technical Research Council (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [215O468]This research was funded by Turkish Scientific and Technical Research Council (TUBITAK) (Project No.: 215O468)

Occurrence and abundance of nematodes on onion in Turkey and their relationship with soil physicochemical properties

WOS:000500527700006The distribution of plant-feeding and free-living nematodes in large scale onion production areas in five geographical regions in Turkey was investigated in 2016 and 2017. Ditylenchus spp. and Tylenchus spp. were widely distributed. The stem and bulb nematode, Ditylenchus dipsaci, was found in 48 locations from 13 provinces. Other plant-feeding nematode genera were Pratylenchus, Paratylenchus and Pratylenchoides. Pratylenchus thornei was the most widely distributed root-lesion nematode species in onion fields in 11 locations from seven provinces. Pratylenchus neglectus was present in three locations and P. vulnus was in four locations. Aphelenchus spp. and Aphelenchoides spp. were the principal fungal-feeding nematodes in onion-growing areas. The most abundant bacterial-feeding nematode genera were Acrobeloides, Cephalobus, Eucephalobus and Rhabditis. Acrobeles and Wilsonema genera were low in occurrence and abundance. Nematodes from Dorylaimida and predator nematodes, Mononchus spp., were also found. The numbers of Ditylenchus from plant samples were significantly correlated positively to silt content, and significantly correlated negatively to organic matter and calcium content.Turkish Scientific and Technical Research Council (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [215O468]This research has been financially supported by Turkish Scientific and Technical Research Council (TUBITAK) (Project no: 215O468)