ACTIVAGE - D9.6 Final results report & sustainability plan (I to IX)

We have reached the end of a challenging and rewarding journey, after 45 months of ACTIVAGE, this is the last report that aims at providing a taste of the huge work done by the teams at the local sites. This is a testimony of the more than 45 partners that have participated in this WP. We started with 9 DS in 7 countries and we finish with 12 DS in 9 countries, having incorporated three new DS through the open call, and expanding ACTIVAGE vision and ecosystem to Portugal and Bulgaria. We have been able to reach the target of deploying AHA-IoT solutions for more than 7.200 users, we hit the mark of 7.776 and almost 97% of the expected deployments. All DS have been able to perform the evaluation, at local and global level, generating the evidence that ACTIVAGE vision is pertinent, relevant, and effective. Details are provided in the individual results and in D6.5. By the end of the project, still 80% of participants continue operation, and almost all DS have in place sustainability plans to continue providing ACTIVAGE services in one way or another. Even in the cases where no clear plans have been possible, there are opportunities for exploiting the services and the knowledge gained in the project

A new method for assaying propantheline and its degradation product, xanthene-9- carboxylic acid using high performance liquid chromatography

A rapid, specific, and precise high-performance liquid chromatographic method is described for the simultaneous analysis of propantheline bromide and its hydrolysis product, xanthene-9-carboxylic acid. Reversed-phase chromatography was conducted using a mobile phase of 40:60, acetonitrile-0.05 M phosphate buffer (pH 2.5) delivered at 2 ml/min. Detection was at 254 nm. Methantheline bromide (internal standard), propantheline bromide, and xanthene-9-carboxylic acid gave retention times of 4.1, 5.4, and 8.3 min, respectively. Within-day, between-day, and total precision (CV) for assay of 15 mg/10 ml propantheline bromide are 1.2, 1.1, and 1.6%, respectively (n = 20). Similar precision was obtained for xanthene-9-carboxylic acid. The limit of detection was 2 ng. The assay is useful for routine quality assurance of propantheline in dosage forms and for stability and kinetic studies