International Journal of Molecular and Clinical Microbiology Association of bcsA and draE genes with biofilm formation on urinary catheter in uropathogenic Escherichia coli isolates

The production of biofilm by E. coli strain can cause serious problem for human health. Catheter is considered to be a suitable place for colonization of microorganisms, so that in 5-10% of patients who used the catheter for only one day and also in all patients who used it for over 28 days, the colonization of bacteria is observed on catheter. The aim of this study was to compare biofilm formation in E. coli and its relationship with pathogenic factors. In this study 144 isolates of uropathogenic E.coli were used in order to compare their potential of biofilm formation on urinary catheter. In order to do this, a piece of the catheter was sliced and put in the tubes containing E. coli suspension, as well as in broth medium (as witness). After incubation at 37 o C for 24 hours, the number of live bacteria involved in biofilm formation was counted by surface cultivation method. The result showed that among the 144 studied E.coli isolates, 130 (89.7%) had cellulose synthesized gene (bcsA), and 22(15.2%) had Dr adhesine gene (draE). There was a significant relationship between bcsA gene and biofilm formation potential on urinary catheter

Effects Of Zno Nanoparticles on Initial Adhesion and fimH Gene Expression Level of Uropathogenic Eschercia coli

Introduction: Uropathogenic Escherichia coli (UPEC) strains are the main causes of urinary tract infections. Adhesion is one of the main and primary steps of UPEC pathogenicity. Type 1 fimbriae is one of the bacterial surface structures that plays an important role in bacterial adhesion. The aim of this study was to evaluate effects of ZnO nanoparticles on the initial adhesion and fimH gene expression level of UPEC. Materials and Method: Four UPEC isolates were used in this study. All isolates were exposed to sub-minimum inhibitory concentration of ZnO nanoparticles (1250 μg/ml). Expression of the fimH gene was evaluated by Real-time PCR. Result: According to the results, presence of nanoparticles reduced the fimH expression level in all four isolates. The highest and lowest rates of down-expression were 1.4-fold and 16.37-fold, respectively. Moreover, these results were consistent with phenotypic observations. Conclusion: However, it is recommended to conduct further studies on gene expression and bacterial adhesion to surfaces to prove whether ZnO nanoparticles could completely prevent UPEC adhesion

Etude de la relation structure-fonction des RNases J chez B. subtilis et du chloroplaste de Chlamydomonas rheinhardtii

PARIS7-Bibliothèque centrale (751132105) / SudocPARIS-BIUP (751062107) / SudocSudocFranceF

Evaluation of the implementation of the nosocomial infection control program in the emergency department of teaching hospitals of Gorgan, Iran

Background and Objective: In hospitals, the emergency department is a complex healthcare environment, and it is necessary to attention to nosocomial infections. This study aimed to evaluate the implementation of nosocomial infection program in the emergency department. Materials and Methods: This descriptive study was conducted in hospitals of Gorgan, Iran, in 2022, using the WHO checklist with 96 questions in 9 section staff; patients, environment, equipment, drug, prevention of infections wound, urinary, respiratory, and blood. Data were collected via observation and interviews. A checklist containing 45 questions was used to determine the impact of staff awareness on the infection control program, in 4 sections oftheoretical knowledge, performing procedures, immunization, and environmental hygiene. Results: The results show that 82.46% of the infection control program was satisfactory. The percentage of scores for different sections of nosocomial infection program including staff, patients, environment, equipment, drug, infection prevention of wound, urinary, respiratory and blood  was 79.31, 77.78,  81.25,  83.34, 100, 44.34, 75.00, 87.88 and 100, respectively. Moreover, the percentage awareness score for emergency medicine specialists, general practitioners, medical interns, and nurses was 100, 95.83, 87.5, and 79.92, respectively. Conclusion: The implementation quality of the infection control program was acceptable and satisfactory. However, some factors were lower than the desired level, which could be dangerous for medical staff, patients, and visitors. The medical staff believed that the support of the senior managers of the University was essential for the implementation of an infection control program, and also holding training courses, and accelerating data sharing

RNase Y, a novel endoribonuclease, initiates riboswitch turnover in Bacillus subtilis

In contrast to Escherichia coli, initiation of mRNA decay in Gram-positive organisms is poorly understood. We studied the fate of the highly structured RNAs generated by premature transcription termination of S-adenosylmethionine (SAM)-dependent riboswitches in Bacillus subtilis. An essential protein of earlier unknown function, YmdA, was identified as a novel endoribonuclease (now called RNase Y) that was capable of preferential cleaving in vitro of the 5′ monophosphorylated yitJ riboswitch upstream of the SAM-binding aptamer domain. Antiterminated full-length yitJ mRNA was not a substrate for RNase Y in vivo and in vitro, transcripts capable of forming the antiterminator were only cleaved in the presence of SAM. Turnover of 10 other SAM-dependent riboswitches was also initiated by RNase Y. Depletion of this ribonuclease increased the half-life of bulk mRNA more than two-fold. This indicates that RNase Y might be not only important for riboswitch RNA turnover but also as a key player in the initiation of mRNA decay in B. subtilis. About 40% of the sequenced eubacterial species have an RNase Y orthologue