A Comparative Study on the Flexural Behaviour of Rubberized and Hybrid Rubberized Reinforced Concrete Beams

This paper aims to investigate the flexural behaviour of the rubberized and hybrid rubberized reinforced concrete beams. A total of fourteen beams, 150×200 mm in cross-section with 1000 mm in length, were subject to a laboratory test over an effective span of 900 mm. The sand river aggregate was replaced by 10%, 12.5%, and 15% of crumb rubber (volume).   The hybrid structure contained two double layers: 1) rubberized reinforcement concrete at the top layer of the beam and 2) reinforcement concrete at the bottom layer of the concrete beam. The static responses by the flexural test of all the beams were evaluated in terms of their fresh properties, failure patterns, total energy, flexural strength, stiffness, and ultimate deflection, modulus of rupture, strain capacity, and ductility index. The results showed that there were improvements when the hybrid beams were used in most cases such as failure pattern, ultimate load, stiffness, modulus of rupture, and stress. The rubberized concrete beams showed improvements in the strain capacity as illustrated in strain gauges and stress-strain curves, toughness, ultimate deflection, and ductility index. The findings of the study revealed an improved performance with the use of the hybrid beams. This has resulted in the implementation of innovative civil engineering applications in the engineering sustainable structures

Performance Evaluation of Path Loss in Mobile Channel for Karada district in Baghdad City

In this work Hata, Lee, Walfisch-Ikegami and Free Space Loss models have been compared with practical path loss based on series of measurements in Karada district in Baghdad for GSM900 downlink band. Hata model showed the closest path loss predictions with mean square error = 10.8 dB, but these results are far from good prediction results and need enhancement. Hata model was optimized using Least Squares method based on measured received signal power. The optimized Hata model showed much better results from the original Hata with mean square error = 6.96 dB. The simulation and calculations were implemented using MATLAB R2009b. The measurements were done using Field Test Display (FTD) with a compatible hand phone to measure signal strength. A Ground Positioning System (GPS) was used to measure the distance from transmitter

Untying a nanoscale knotted polymer structure to linear chains for efficient gene delivery in vitro and to the brain

The purpose of this study was to develop a platform transfection technology, for applications in the brain, which could transfect astrocytes without requiring cell specific functionalization and without the common cause of toxicity through high charge density. Here we show that a simple and scalable preparation technique can be used to produce a “knot” structured cationic polymer, where single growing chains can crosslink together via disulphide intramolecular crosslinks (internal cyclizations). This well-defined knot structure can thus “untie” under reducing conditions, showing a more favorable transfection profile for astrocytes compared to 25 kDa-PEI (48-fold), SuperFect® (39-fold) and Lipofectamine®2000 (18-fold) whilst maintaining neural cell viability at over 80% after four days of culture. The high transfection/lack of toxicity of this knot structured polymer in vitro, combined with its ability to mediate luciferase transgene expression in the adult rat brain, demonstrates its use as a platform transfection technology which should be investigated further for neurodegenerative disease therapies

3D Bioprinting of stimuli-responsive polymers synthesised from DE-ATRP into soft tissue replicas

Synthetic polymers possess more reproducible physical and chemical properties than their naturally occurring counterparts. They have also emerged as an important alternative for fabricating tissue substitutes because they can be molecularly tailored to have vast array of molecular weights, block structures, active functional groups, and mechanical properties. To this date however, there has been very few successful and fully functional synthetic tissue and organ substitutes and with the rapidly spreading 3D printing technology beginning to reshape the tissue engineering and regenerative field, the need for an effective, safe, and bio printable biomaterial is becoming more and more urgent. Here, we have developed a synthetic polymer from controlled living radical polymerization that can be printed into well-defined structures. The polymer showed low cytotoxicity before and after printing. Additionally, the incorporation of gelatine-methacrylate coated PLGA microparticles within the hydrogel provided cell adhesion surfaces for cell proliferation. The results point to possible application of the microparticle seeded, synthetic hydrogel as a direct printable tissue or organ substitute

A Fluorescently Labeled, Hyperbranched Polymer Synthesized from DE-ATRP for the Detection of DNA Hybridization

Journal articleThe early detection of oligonucleotide biomarkers of disease, such as microRNAs, has been established as a fundamental factor in cancer diagnosis. As the levels of these small molecules (microRNAs) in blood have recently been found to be significantly affected in cancer patients, they offer a means of early stage detection of cancer. Towards the goal of creating a novel method of DNA hybridization detection, we report the detection of specific sequences of small oligonucleotides in a model experiment carried out in serum. The results shown here display the versatility of the DE-ATRP method in synthesizing a specific polymer structure capable of changing its physical properties in the presence of double stranded DNA. The polymer was labeled and used to detect single-stranded DNA in serum successfully.peer-reviewe

Untying a nanoscale knotted polymer structure to linear chains for efficient gene delivery in vitro and to the brain

The purpose of this study was to develop a platform transfection technology, for applications in the brain, which could transfect astrocytes without requiring cell specific functionalization and without the common cause of toxicity through high charge density. Here we show that a simple and scalable preparation technique can be used to produce a "knot" structured cationic polymer, where single growing chains can crosslink together via disulphide intramolecular crosslinks (internal cyclizations). This well-defined knot structure can thus "untie" under reducing conditions, showing a more favorable transfection profile for astrocytes comp-red to 25 kDa-PEI (48-fold), SuperFect (R) (39-fold) and Lipofectamine (R) 2000 (18-fold) whilst maintaining neural cell viability at over 80% after four days of culture. The high transfection/lack of toxicity of this knot structured polymer in vitro, combined with its ability to mediate luciferase transgene expression in the adult rat brain, demonstrates its use as - platform transfection technology which should be investigated further for neurodegenerative disease therapies

Toxicological features of catha edulis (Khat) on livers and kidneys of male and female sprague-dawley rats: a subchronic study

Hepato- and nephrotoxicity of Khat consumption (Catha edulis Forskal) have been evoked. Therefore, this study was conducted to evaluate such possible hepatorenal toxicity in female and male Sprague-Dawley rats (SD rats) focusing primarily on liver and kidney. In addition, female and male rats were investigated separately. Accordingly, forty-eight SD-rats (100-120 g) were distributed randomly into four groups of males and female (n = 12). Normal controls (NCs) received distilled water, whereas test groups received 500 mg/kg (low dose (LD)), 1000 mg/kg (medium dose (MD)), or 2000 mg/kg (high dose (HD)) of crude extract of Catha edulis orally for 4 weeks. Then, physical, biochemical, hematological, and histological parameters were analyzed. Results in Khat-fed rats showed hepatic enlargement, abnormal findings in serum aspartate aminotransferase (AST), and alkaline phosphatase (ALP) of male and female SD-rats and serum albumin (A) and serum creatinine (Cr) of female as compared to controls. In addition, histopathological abnormalities confirmed hepatic and renal toxicities of Khat that were related to heavy Khat consumption. In summary, Khat could be associated with hepatic hypertrophy and hepatotoxicity in male and female SD-rats and nephrotoxicity only in female SD-rats