Dietary Intake, Serum Hormone Concentrations, Amenorrhea and Bone Mineral Density of Physique Athletes and Active Gym Enthusiasts

As the diet, hormones, amenorrhea, and bone mineral density (BMD) of physique athletes (PA) and gym enthusiasts (GE) are little-explored, we studied those in 69 females (50 PA, 19 GE) and 20 males (11 PA, 9 GE). Energy availability (EA, kcal·kgFFM−1·d−1 in DXA) in female and male PA was ~41.3 and ~37.2, and in GE ~39.4 and ~35.3, respectively. Low EA (LEA) was found in 10% and 26% of female PA and GE, respectively, and in 11% of male GE. In PA, daily protein intake (g/kg body mass) was ~2.9–3.0, whereas carbohydrate and fat intakes were ~3.6–4.3 and ~0.8–1.0, respectively. PA had higher protein and carbohydrate and lower fat intakes than GE (p < 0.05). Estradiol, testosterone, IGF-1, insulin, leptin, TSH, T4, T3, cortisol, or BMD did not differ between PA and GE. Serum IGF-1 and leptin were explained 6% and 7%, respectively, by EA. In non-users of hormonal contraceptives, amenorrhea was found only in PA (27%) and was associated with lower fat percentage, but not EA, BMD, or hormones. In conclusion, off-season dietary intakes, hormone levels, and BMD meet the recommendations in most of the PA and GE. Maintaining too-low body fat during the off-season may predispose to menstrual disturbances

Dietary Intake, Serum Hormone Concentrations, Amenorrhea and Bone Mineral Density of Physique Athletes and Active Gym Enthusiasts

As the diet, hormones, amenorrhea, and bone mineral density (BMD) of physique athletes (PA) and gym enthusiasts (GE) are little-explored, we studied those in 69 females (50 PA, 19 GE) and 20 males (11 PA, 9 GE). Energy availability (EA, kcal·kgFFM−1·d−1 in DXA) in female and male PA was ~41.3 and ~37.2, and in GE ~39.4 and ~35.3, respectively. Low EA (LEA) was found in 10% and 26% of female PA and GE, respectively, and in 11% of male GE. In PA, daily protein intake (g/kg body mass) was ~2.9–3.0, whereas carbohydrate and fat intakes were ~3.6–4.3 and ~0.8–1.0, respectively. PA had higher protein and carbohydrate and lower fat intakes than GE (p < 0.05). Estradiol, testosterone, IGF-1, insulin, leptin, TSH, T4, T3, cortisol, or BMD did not differ between PA and GE. Serum IGF-1 and leptin were explained 6% and 7%, respectively, by EA. In non-users of hormonal contraceptives, amenorrhea was found only in PA (27%) and was associated with lower fat percentage, but not EA, BMD, or hormones. In conclusion, off-season dietary intakes, hormone levels, and BMD meet the recommendations in most of the PA and GE. Maintaining too-low body fat during the off-season may predispose to menstrual disturbances

Priming the Motor Cortex With Anodal Transcranial Direct Current Stimulation Affects the Acute Inhibitory Corticospinal Responses to Strength Training

Synaptic plasticity in the motor cortex (M1) is associated with strength training and can be modified by transcranial direct current stimulation (tDCS). The M1 responses to strength training increase when anodal-tDCS is applied during training due to gating. An additional approach to improve the M1 responses to strength training, which has not been explored, is to use anodal-tDCS to prime the M1 before a bout of strength training. We examined the priming effects of anodal-tDCS of M1 on the acute corticospinal responses to strength training. In a randomized double-blinded cross-over design, changes in isometric strength, corticospinal-excitability and inhibition (assessed as area under the recruitment curve [AURC] using transcranial magnetic stimulation [TMS]) were analysed in 13 adults exposed to 20-min of anodal and sham-tDCS followed by a strength training session of the right elbow-flexors. We observed a significant decrease in isometric elbow-flexor strength immediately following training (11-12%; P < 0.05) which was not different between anodal-tDCS and sham-tDCS. TMS revealed a 24% increase in AURC for corticospinal-excitability following anodal-tDCS and strength training; this increase was not different between conditions. However, there was a 14% reduction in AURC for corticospinal inhibition when anodal-tDCS was applied prior to strength training when compared to sham-tDCS and strength training (all P < 0.05). Priming anodal-tDCS had a limited effect in facilitating corticospinal-excitability following an acute bout of strength training. nterestingly, the interaction of anodal-tDCS and strength training appears to affect the excitability of intracortical inhibitory circuits of the M1 via non-homeostatic mechanisms

Resistance Training Acutely Impairs Agility and Spike-Specific Performance Measures in Collegiate Female Volleyball Players Returning from the Off-Season

[EN]This study examined the acute effects of resistance training (RT) on volleyball-specific performance. Sixteen female volleyball players undertook their initial, pre-season RT bout. Countermovement jump (CMJ), delayed onset of muscle soreness (DOMS), and sport-specific performances (i.e., run-up jump, agility, and spiking speed and accuracy) were measured before, 24 (T24), and 48 (T48) hours after RT. A significant increase in DOMS was observed at T24 and T48 (~207.6% ± 119.3%; p < 0.05; ES = 1.8 (95% CI: 0.94–2.57)), whilst agility was significantly impaired at T48 (1.7% ± 2.5%; p < 0.05; ES = 0.30 (95% CI: −0.99–0.40)). However, there were no differences in CMJ (~−2.21% ± 7.6%; p > 0.05; ES = −0.11 (95% CI: −0.80–0.58)) and run-up jump (~−1.4% ± 4.7%; p > 0.05; ES = −0.07 (95% CI: −0.76–0.63)). Spiking speed was significantly reduced (−3.5% ± 4.4%; p < 0.05; ES = −0.28 (95% CI: −0.43–0.97)), although accuracy was improved (38.3% ± 81.4%: p < 0.05) at T48. Thus, the initial, preseason RT bout compromised agility and spiking speed for several days post-exercise. Conversely, spiking accuracy improved, suggesting a speed–accuracy trade-off. Nonetheless, at least a 48-h recovery may be necessary after the initial RT bout for athletes returning from the off-season or injury

Immunoscore in mismatch repair-proficient and -deficient colon cancer

The aim of this study was to investigate immune response and its prognostic significance in colon carcinomas using the previously described Immunoscore (IS). A population-based series of 779 colorectal cancers, operated on between 2000 and 2010, were classified according to tumour, node, metastasis (TNM) status, mismatch repair (MMR), and BRAF mutation status. Rectal cancer cases (n = 203) were excluded as a high proportion of these patients received preoperative neoadjuvant chemoradiotherapy. Tissue microarray (TMA) samples collected from the tumour centre and invasive front were immunostained for CD3 and CD8. Lymphocytes were then digitally calculated to categorize IS from grade 0 to 4. Samples adequate for IS were available from 510 tumours. IS was significantly associated with AJCC/UICC stage, T stage, lymph node and distant metastases, perineural and lymphovascular invasion, MMR status, and BRAF mutation status. For IS0, IS1, IS2, IS3 and IS4, respectively, the 5-year disease-free survival (DFS) rates were 59, 68, 78, 83 and 94% (p Peer reviewe

The Effects of Intensive Weight Reduction on Body Composition and Serum Hormones in Female Fitness Competitors

Worries about the potential negative consequences of popular fat loss regimens for aesthetic purposes in normal weight females have been surfacing in the media. However, longitudinal studies investigating these kinds of diets are lacking. The purpose of the present study was to investigate the effects of a 4-month fat-loss diet in normal weight females competing in fitness-sport. In total 50 participants finished the study with 27 females (27.2 +/- 4.1 years) dieting for a competition and 23 (27.7 +/- 3.7 years) acting as weight-stable controls. The energy deficit of the diet group was achieved by reducing carbohydrate intake and increasing aerobic exercise while maintaining a high level of protein intake and resistance training in addition to moderate fat intake. The diet led to a similar to 12% decrease in body weight (P <0.001) and a similar to 3550% decrease in fat mass (DXA, bioimpedance, skinfolds, P <0.001) whereas the control group maintained their body and fat mass (diet x group interaction P <0.001). A small decrease in lean mass (bioimpedance and skinfolds) and in vastus lateralis muscle cross-sectional area (ultrasound) were observed in diet (P <0.05), whereas other results were unaltered (DXA: lean mass, ultrasound: triceps brachii thickness). The hormonal system was altered during the diet with decreased serum concentrations of leptin, triiodothyronine (T3), testosterone (P <0.001), and estradiol (P <0.01) coinciding with an increased incidence of menstrual irregularities (P <0.05). Body weight and all hormones except T3 and testosterone returned to baseline during a 34 month recovery period including increased energy intake and decreased levels aerobic exercise. This study shows for the first time that most of the hormonal changes after a 3550% decrease in body fat in previously normal-weight females can recover within 34 months of increased energy intake.Peer reviewe

Molecular Pathways Mediating Immunosuppression in Response to Prolonged Intensive Physical Training, Low-Energy Availability, and Intensive Weight Loss

Exercise and exercise-induced weight loss have a beneficial effect on overall health, including positive effects on molecular pathways associated with immune function, especially in overweight individuals. The main aim of our study was to assess how energy deprivation (i.e., "semi-starvation") leading to substantial fat mass loss affects the immune system and immunosuppression in previously normal weight individuals. Thus, to address this hypothesis, we applied a high-throughput systems biology approach to better characterize potential key pathways associated with immune system modulation during intensive weight loss and subsequent weight regain. We examined 42 healthy female physique athletes (age 27.5 +/- 4.0 years, body mass index 23.4 +/- 1.7 kg/m(2)) volunteered into either a diet group (n = 25) or a control group (n = 17). For the diet group, the energy intake was reduced and exercise levels were increased to induce loss of fat mass that was subsequently regained during a recovery period. The control group was instructed to maintain their typical lifestyle, exercise levels, and energy intake at a constant level. For quantification of systems biology markers, fasting blood samples were drawn at three time points: baseline (PRE), at the end of the weight loss period (MID 21.1 +/- 3.1 weeks after PRE), and at the end of the weight regain period (POST 18.4 +/- 2.9 weeks after MID). In contrast to the control group, the diet group showed significant (false discovery ratePeer reviewe

Effects of upper body eccentric versus concentric strength training and detraining on maximal force, muscle activation, hypertrophy and serum hormones in women

Effects of eccentric (ECC) versus concentric (CON) strength training of the upper body performed twice a week for 10 weeks followed by detraining for five weeks on maximal force, muscle activation, muscle mass and serum hormone concentrations were investigated in young women (n = 11 and n = 12). One-repetition bench press (1RM), maximal isometric force and surface electromyography (EMG) of triceps brachii (TB), anterior deltoid (AD) and pectoralis major (PM), cross-sectional area (CSA) of TB (Long (LoH) and Lateral Head (LaH)) and thickness of PM, as well as serum concentrations of free testosterone, cortisol, follicle-stimulating hormone, estradiol and sex hormone-binding globulin were measured. ECC and CON training led to increases of 17.2 ± 11.3% (p \u3c 0.001) and 13.1 ± 5.7% (p \u3c 0.001) in 1RM followed by decreases of-6.6 ± 3.6% (p \u3c 0.01) and-8.0 ± 4.5% (p \u3c 0.001) during detraining, respectively. Isometric force increased in ECC by 11.4 ± 9.6 % (p \u3c 0.05) from week 5 to 10, while the change in CON by 3.9±6.8% was not significant and a between group difference was noted (p \u3c 0.05). Maximal total integrated EMG of trained muscles increased only in the whole subject group (p \u3c 0.05). CSA of TB (LoH) increased in ECC by 8.7 ± 8.0% (p \u3c 0.001) and in CON by 3.4 ± 1.6% (p \u3c 0.01) and differed between groups (p \u3c 0.05), and CSA of TB (LaH) in ECC by 15.7 ± 8.0% (p \u3c 0.001) and CON by 9.7 ± 6.6% (p \u3c 0.001). PM thickness increased in ECC by 17.7 ± 10.9% (p \u3c 0.001) and CON by 14.0 ± 5.9% (p \u3c 0.001). Total muscle sum value (LoH + LaH + PM) increased in ECC by 12.4 ± 6.9% (p \u3c 0.001) and in CON by 7.1 ± 2.9% (p \u3c 0.001) differing between groups (p \u3c 0.05) and decreased during detraining in ECC by-6.5 ± 4.3% (p \u3c 0.001) and CON by-6.1 ± 2.8% (p \u3c 0.001). The post detraining combined sum value of CSA and thickness was in ECC higher (p \u3c 0.05) than at pre training. No changes were detected in serum hormone concentrations, but baseline free testosterone levels in the ECC and CON group combined correlated with changes in 1RM (r = 0.520, p \u3c 0.016) during training. Large neuromuscular adaptations of the upper body occurred in women during ECC, and CON training in 10 weeks. Isometric force increased only in response to ECC, and total muscle sum value increased more during ECC than CON training. However, no changes occurred in serum hormones, but individual serum-free testosterone baseline concentrations correlated with changes in 1RM during strength training in the entire group. Both groups showed significant decreases in neuromuscular performance and muscle mass during detraining, while post detraining muscle sum value was only in ECC significantly higher than at pre training

Kohti digitaalista ja älykästä rautatieliikennettä : Digirata-valmisteluvaiheen loppuraportti

VALMISTELUVAIHE Suomen rautatieliikenteen kasvun ja kilpailukyvyn mahdollistaa, Digirata-selvityksessä linjattu, radioverkkoteknologiaan perustuva ETCS. ETCS korvaa nykyiset junien kulkua turvaavat järjestelmät sekä mahdollistaa rautateiden digitalisaation. Digiradan valmisteluvaiheessa matka kohti modernia rautatieliikennettä on alkanut. Vaikka eurooppalaisen sääntelyn kehitys voi tuoda matkalle haasteita, tulee Suomi Digirata-hankkeen myötä olemaan merkittävä tekijä kehityskulussa kohti digitalisaatiota ja toimimaan sunnannäyttäjänä esimerkiksi rautateiden radioverkkokehityksessä. Tavoiteltava teknologia on vielä osittain kehityksessä, minkä vuoksi Digiradan seuraavat vaiheet ovat kriittisen tärkeitä onnistumisen varmistamiseksi. Tavoite on asetettu kunnianhimoiseksi kansainvälisestikin tarkasteltuna. Rautateiltä vaaditaan tulevaisuudessa entistä enemmän ketteryyttä ja joustavuutta – Digiradan mahdollistama kuljetusmäärien merkittävä lisääminen ja matkaketjujen tehostaminen rautateillä parantaisi myös Suomen kansainvälistä kilpailukykyä. Turvallisuus on myös erittäin tärkeä osa rautatieliikennettä ja se on keskeinen tekijä Digiradan ytimessä. Matka kohti muutosta on aloitettu ja osa kipupisteistä on jo tunnistettu. Haasteeseen vastaa yhtenäinen rautatiesektori, joka rakentaa tehokkaan vastauksen tulevaisuuden kuljetustarpeisiin. Yhteyshenkilöt liikenne- ja viestintäministeriössä: Suvi Kankare, puh. 0295 34 2105, [email protected] Katariina Säynäjärvi, puh. 0295 34 2029, [email protected] Janne Hauta, puh. 0295 34 2322, [email protected]

Increased cross-education of muscle strength and reduced corticospinal inhibition following eccentric strength training

AIM: Strength training of one limb results in a substantial increase in the strength of the untrained limb, however, it remains unknown what the corticospinal responses are following either eccentric or concentric strength training and how this relates to the cross-education of strength. The aim of this study was to determine if eccentric or concentric unilateral strength training differentially modulates corticospinal excitability, inhibition and the cross-transfer of strength. METHODS: Changes in contralateral (left limb) concentric strength, eccentric strength, motor-evoked potentials, short-interval intracortical inhibition and silent period durations were analyzed in groups of young adults who exercised the right wrist flexors with either eccentric (N=9) or concentric (N=9) contractions for 12 sessions over 4weeks. Control subjects (N=9) did not train. RESULTS: Following training, both groups exhibited a significant strength gain in the trained limb (concentric group increased concentric strength by 64% and eccentric group increased eccentric strength by 62%) and the extent of the cross-transfer of strength was 28% and 47% for the concentric and eccentric group, respectively, which was different between groups (P=0.031). Transcranial magnetic stimulation revealed that eccentric training reduced intracortical inhibition (37%), silent period duration (15-27%) and increased corticospinal excitability (51%) compared to concentric training for the untrained limb (P=0.033). There was no change in the control group. CONCLUSION: The results show that eccentric training uniquely modulates corticospinal excitability and inhibition of the untrained limb to a greater extent than concentric training. These findings suggest that unilateral eccentric contractions provide a greater stimulus in cross-education paradigms and should be an integral part of the rehabilitative process following unilateral injury to maximize the response