Fractionation of acacia honey affects its antioxidant potential in vitro

Objective: To investigate the effects of fractionation of acacia honey on its antioxidant potential in contrast with the pure honey from whole blood, brain and liver in vitro. Methods: Honey was partitioned into three fractions (dichloromethane, ethyl acetate and aqueous). Their immuno-modulatory effect on whole blood was assayed using Luminol-amplified chemiluminescence technique. Their antioxidant activities on rat brain and hepatic tissues which covers for catalase, SOD activities and lipid peroxidation. Results: Fractions of the honey enhanced the production of radicals with no significant (P>0.05) antioxidant activity on whole blood where as pure honey does. Pure honey significantly (P0.05) effects on lipid peroxidation. Conclusions: Fractionation of acacia honey negatively affects its antioxidant potential thereby making it a radical generating agent in contrast with the unfractionated