Bacterial blight disease caused by Pseudomonas cichorii on chrysanthemum in Egypt

Chrysanthemum (Dendranthema grandiflorum) is one of the important cut flowers and pot plants which belong to family Asteraceae. The aim of this study is to isolate and identify causal pathogens of Chrysanthemum bacterial blight disease, samples showing typical symptoms of bacterial blight disease on leaves, buds, stems or flowers obtained from different geographical areas of Egypt were used. The most conspicuous symptoms in infected leaves appear as water-soaked spots then become brown and dry. Ten bacterial isolates were isolated from different parts of Chrysanthemum which collected from different localities. In this respect, the bacterial isolates designated as Wl-1 and Wb-2 which were isolated from leaves and buds respectively of chrysanthemum white variety in Qualubia (Moshtohor). Meanwhile, the isolates coded as Ps-3 and Pf-4 were isolated from stems and flowers of chrysanthemum purple variety respectively in the same governorate (El-kanater El-khairia), the bacterial isolates coded as Ws-5 and Wf-6 were isolated from stems and flowers of chrysanthemum white variety planted in Menoufia governorate (Shebeen El-kom). While, the isolates coded as Yl-7 and Yb-8 were isolated from leaves and buds of chrysanthemum yellow variety respectively in Giza governorate (Kirdasah). The isolate Rs- 9 and Rf-10 were isolated from stems and flowers of chrysanthemum red variety from the same governorate (Elmariotia). Identification of isolated bacteria using the traditional techniques according to their inspected morphological, cultural characteristics, biochemical and physiological characteristics, these traditional tests revealed that these isolates may be belong to three genera i.e., Wl-1, Pf-4 and Rs-9 could be identified as Pseudomonas cichorii, while, the isolate Yb-8 could be identified as Pseudomonas fluorescens while the isolate Wf-6 was identified as Bacillus subtilis. The present study examined the level of genetic diversity and its molecular variation of the bacterial blight disease caused by Pseudomonas cichorii, three isolates from five geographic regions in Egypt. In addition, the isolates were pathogenic to eight plants from different plant families by artificial inoculations. This bacterium has a wide host range and this work is important for cataloging plant pathogenic bacteria that occur throughout special conditions may become epidemic in Egypt

Novel Antiviral and Antibacterial Durable Polyester Fabrics Printed with Selenium Nanoparticles (SeNPs)

The COVID-19 pandemic has clearly shown the importance of developing advanced protective equipment, and new antiviral fabrics for the protection and prevention of life-threatening viral diseases are needed. In this study, selenium nanoparticles (SeNPs) were combined with polyester fabrics using printing technique to obtain multifunctional properties, including combined antiviral and antibacterial activities as well as coloring. The properties of the printed polyester fabrics with SeNPs were estimated, including tensile strength and color fastness. Characterization of the SeNPs was carried out using TEM and SEM. The results of the analysis showed good uniformity and stability of the particles with sizes range from 40–60 nm and 40–80 nm for SeNPs 25 mM and 50 mM, respectively, as well as uniform coating of the SeNPs on the fabric. In addition, the SeNPs—printed polyester fabric exhibited high disinfection activity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with an inhibition percentage of 87.5%. Moreover, a toxicity test of the resulting printed fabric revealed low cytotoxicity against the HFB4 cell line. In contrast, the treated fabric under study showed excellent killing potentiality against Gram-positive bacteria (Bacillus cereus) and Gram-negative bacteria (Pseudomonas aeruginosa, Salmonella typhi, and Escherichia coli). This multifunctional fabric has high potential for use in protective clothing applications by providing passive and active protection pathways

Computer simulation for the seismic behaviour of bridge expansion joints enhanced with SMA: Case study

This paper concerns the study of the expansion joints of Benha Bridge under seismic loads. The seismic behaviour of its bridge expansion joints has been enhanced by shape memory alloy (SMA) dampers as a form of energy-dissipation device. SMA can dissipate a part of the input energy from the earthquake by generating a counterforce for the induced seismic force, allowing the opportunity to control the joint width and overcome pounding and unseating problems. In this research, MATLAB programs are developed to calculate the joint width responses in the two cases of without SMA and with SMA control devices embedded between adjacent bridge frames, which are modeled as a single degree of freedom system for each frame. Benha Bridge is the subject of this case study, which aims to check and validate the SMA parameter values that their charts have introduced from earlier research and to improve the seismic behaviour of bridge expansion joints by eliminating unseating and pounding problems. The results show that SMA dampers are able to control the joint width and absorb the excess forces caused by nearby expansion joint overlap. Furthermore, SMA design charts are applicable for bridges of multiple frames when they are homogenous in time periods

Anti-Alzheimer Activity of Combinations of Cocoa with Vinpocetine or Other Nutraceuticals in Rat Model: Modulation of Wnt3/β-Catenin/GSK-3β/Nrf2/HO-1 and PERK/CHOP/Bcl-2 Pathways

Alzheimer’s disease (AD) is a devastating illness with limited therapeutic interventions. The aim of this study is to investigate the pathophysiological mechanisms underlying AD and explore the potential neuroprotective effects of cocoa, either alone or in combination with other nutraceuticals, in an animal model of aluminum-induced AD. Rats were divided into nine groups: control, aluminum chloride (AlCl3) alone, AlCl3 with cocoa alone, AlCl3 with vinpocetine (VIN), AlCl3 with epigallocatechin-3-gallate (EGCG), AlCl3 with coenzyme Q10 (CoQ10), AlCl3 with wheatgrass (WG), AlCl3 with vitamin (Vit) B complex, and AlCl3 with a combination of Vit C, Vit E, and selenium (Se). The animals were treated for five weeks, and we assessed behavioral, histopathological, and biochemical changes, focusing on oxidative stress, inflammation, Wnt/GSK-3β/β-catenin signaling, ER stress, autophagy, and apoptosis. AlCl3 administration induced oxidative stress, as evidenced by elevated levels of malondialdehyde (MDA) and downregulation of cellular antioxidants (Nrf2, HO-1, SOD, and TAC). AlCl3 also upregulated inflammatory biomarkers (TNF-α and IL-1β) and GSK-3β, leading to increased tau phosphorylation, decreased brain-derived neurotrophic factor (BDNF) expression, and downregulation of the Wnt/β-catenin pathway. Furthermore, AlCl3 intensified C/EBP, p-PERK, GRP-78, and CHOP, indicating sustained ER stress, and decreased Beclin-1 and anti-apoptotic B-cell lymphoma 2 (Bcl-2) expressions. These alterations contributed to the observed behavioral and histological changes in the AlCl3-induced AD model. Administration of cocoa, either alone or in combination with other nutraceuticals, particularly VIN or EGCG, demonstrated remarkable amelioration of all assessed parameters. The combination of cocoa with nutraceuticals attenuated the AD-mediated deterioration by modulating interrelated pathophysiological pathways, including inflammation, antioxidant responses, GSK-3β-Wnt/β-catenin signaling, ER stress, and apoptosis. These findings provide insights into the intricate pathogenesis of AD and highlight the neuroprotective effects of nutraceuticals through multiple signaling pathways

Impact of the International Nosocomial Infection Control Consortium (INICC)’s multidimensional approach on rates of ventilator-associated pneumonia in intensive care units in 22 hospitals of 14 cities of the Kingdom of Saudi Arabia

To analyze the impact of the International Nosocomial Infection Control Consortium (INICC) Multidimensional Approach (IMA) and use of INICC Surveillance Online System (ISOS) on ventilator-associated pneumonia (VAP) rates in Saudi Arabia from September 2013 to February 2017. A multicenter, prospective, before–after surveillance study on 14,961 patients in 37 intensive care units (ICUs) of 22 hospitals. During baseline, we performed outcome surveillance of VAP applying the definitions of the CDC/NHSN. During intervention, we implemented the IMA and the ISOS, which included: (1) a bundle of infection prevention practice interventions, (2) education, (3) outcome surveillance, (4) process surveillance, (5) feedback on VAP rates and consequences and (6) performance feedback of process surveillance. Bivariate and multivariate regression analyses were performed using generalized linear mixed models to estimate the effect of intervention. The baseline rate of 7.84 VAPs per 1000 mechanical-ventilator (MV)-days―with 20,927 MV-days and 164 VAPs―, was reduced to 4.74 VAPs per 1000 MV-days―with 118,929 MV-days and 771 VAPs―, accounting for a 39% rate reduction (IDR 0.61; 95% CI 0.5–0.7; P 0.001). Implementing the IMA was associated with significant reductions in VAP rates in ICUs of Saudi Arabia