1 research outputs found
Spatial distribution, enzymatic activity, and insecticide resistance status of Aedes aegypti and Aedes albopictus from dengue hotspot areas in Kuala Lumpur and Selangor, Malaysia
The effectiveness of insecticide-based dengue control interventions is very much influenced
by the insecticide resistance status of the mosquito at the targeted areas. This study aims to
determine the insecticide resistance status and the enzymatic activity. WHO adult bioassays
conducted on Ae. aegypti and Ae. albopictus from 12 dengue hotspots outbreak areas in Kuala
Lumpur and Selangor towards insecticides currently and historically used for mosquito control
in Malaysia which include two pyrethroids, one organoclorine, one organophosphate and one
carbamate. Biochemical enzyme assays were conducted and the activity of enzymes α�Esterase, MFO, GST and AChE were examined. Kruskal-Wallis H, Mann-Whitney U and
ANOVA test were used to determine the significant difference of the mortality between
insecticides and localities, the enzymes activity between the field and the lab strains, and the
enzymes activity within all field strains. Ae. aegypti from all sites have developed resistance
towards all tested insecticides based on WHO adult bioassays; permethrin, DDT, malathion
and propoxur. The result of biochemical enzyme assays demonstrated that the activity of
enzymes was altered. α-esterase and MFO were altered in both species from all areas. GST was
altered in both species as well except in Ae. albopictus from sites Bandar Rinching and Taman
Gombak Setia. AChE was found significantly demoted in Ae. aegypti from Sri Nilam Shah
Alam and Ae. albopictus from Flat Sri Labuan Cheras only. The resistance detected might be
the result of activity by either single or several enzymes combined. The development of
resistance is mainly via metabolic mechanism