Pendampingan Budidaya Pisang Berkelanjutan dan Manajemen Hama Penyakit di Sindangsari Kabupaten Serang

Banana is a native fruit of Indonesia, grown in all of the Indonesian archipelagos. Most of the national banana production was produced by small-scale and household farmers. Banten is the fifth largest province for national banana producers. Nevertheless, no region was developed only for bananas as the main crop. Pengabdian Kepada Masyarakat program aimed to give knowledge and guidance in sustainable banana cultivation and pest disease management on the banana to Farmers at Desa Sindangsari Banten. It is also the initial program of Universitas Sultan Ageng Tirtayasa to create a banana village in Banten. The program started with a survey of the socio-economy of people in the village, identification of local banana varieties, and pest disease incidence of bananas on the farm. Then, a Focus group discussion on sustainable banana cultivation and pest disease management was conducted, followed by a demonstration of how to plant bananas in good practice. At the end of the program, farmers were given two commercial varieties, Cavendish and Barangan, to be grown on their farms. Farmers follow the discussion enthusiastically, asking questions and describing the current state of their banana cultivation. Sustainable banana cultivation practices could be used in the village to improve the quality and production of household farmers' bananas. Thus, it will increase the value of the banana in the market and raise the farmers' income. Finally, it will promote bananas as the leading local commodity and make Sindangsari a banana village in Banten.Banana is a native fruit of Indonesia, grown in all of the Indonesian archipelagos. Most of the national banana production was produced by small-scale and household farmers. Banten is the fifth largest province for national banana producers. Nevertheless, no region was developed only for bananas as the main crop. Pengabdian Kepada Masyarakat program aimed to give knowledge and guidance in sustainable banana cultivation and pest disease management on the banana to Farmers at Desa Sindangsari Banten. It is also the initial program of Universitas Sultan Ageng Tirtayasa to create a banana village in Banten. The program started with a survey of the socio-economy of people in the village, identification of local banana varieties, and pest disease incidence of bananas on the farm. Then, a Focus group discussion on sustainable banana cultivation and pest disease management was conducted, followed by a demonstration of how to plant bananas in good practice. At the end of the program, farmers were given two commercial varieties, Cavendish and Barangan, to be grown on their farms. Farmers follow the discussion enthusiastically, asking questions and describing the current state of their banana cultivation. Sustainable banana cultivation practices could be used in the village to improve the quality and production of household farmers' bananas. Thus, it will increase the value of the banana in the market and raise the farmers' income. Finally, it will promote bananas as the leading local commodity and make Sindangsari a banana village in Banten

Genetic Diversity of Musa balbisiana Colla in Indonesia Based on AFLP Marker

Musa balbisiana Colla, known as Pisang Klutuk/Pisang Batu, is important for banana breeding program due to its B genome and often considered  to be resistant to pest, disease and drought.  Banana is a big and tall herb up to 7-9 m in height.The study was conducted to assess genetic diversity of 21 accessions of M. balbisiana cultivars/varieties from Indonesia based on Amplified Fragment Length Polymorphism (AFLP) markers in order to provide basic information to support banana breeding program. Analyses of similarity index of Nei and Li and clustering based on UPGMA was conducted using NTSYS program. Principal Component Analysis (PCA) was conducted by MINITAB 14. Twenty two primer pairs of AFLP markers produced 485 AFLP bands varying from 51-3206 bp in size and 46.18% of the AFLP bands were polymorphic. Genetic diversity among the 21 accessions of M. balbisiana was 13.8%. The genetic diversity of wild M. balbisiana was 12.9%, higher than the genetic diversity of cultivated M. balbisiana which was 11.5%. Cluster analysis based on UPGMA suggested that wild M. balbisiana and cultivated M. balbisiana could not be separated into different clusters. Both cluster analysis and PCA produced the same three groups of the accessions. The PCA analyses showed that 17 AFLP bands were responsible for the grouping

ANALISIS KERAGAMAN GENETIK Musa balbisiana Colla BERDASARKAN MARKA RAPD DAN ISSR

Wild Musa balbisiana Colla is one of the progenitors of cultivated bananas and plantains. It is originated in Asia, and distributed from India to Papua New Guinea. This study was conducted to assess the molecular diversity of 25 accessions of M. balbisiana based on Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) analyses. RAPD and ISSR fingerprints of these banana varieties were detected by amplifications of nine primers of RAPDs and six primers of ISSRs. RAPD primers produced 84 amplified fragments varying from 150 bp to 2300 bp in size. 21.43 % of the amplification bands were polymorphic. ISSR primers produced 61 amplified fragments varying from 250 bp to 2200 bp in size. 29.30 % of the amplification bands were polymorphic. Based on these results, the 25 accessions of Indonesian M. balbisiana showed a low genetic variation, with coefficient of similarity ranged from 0.81 to 0.99

Penampilan Random Amplified Polymorphic Dna Pada Azadirachta Indica a. Juss Dari Taman Nasional Baluran

Azadirachta indica A. Juss (Apocynaceae) is a large tree of the lowland tropical rain forest of Southeast Asia that occurs in Thailand, the Malay Peninsula, on the island of Java (East Java) and Lesser Sunda Islands. Its economic value was in its wood (timber), and as medicinal plant. The information on genetic diversity of the species is very limited. Hence studies were initiated and genetic diversity estimated using RAPD markers in 27 accessions of A.indica procured from three geographical regions of TN Baluran and Balai Litbang Kehutanan. Seven selected Operon primers (10 mer) generated a total of 133 consistent amplification products ranging from 132 bp to 5.6 Kb. The cluster analysis separated the 27 individuals into 2 clusters. The range of genetic dissimilarityvalue among samples was from 0.07 to 0.33, while genetic distance among populations was from 0.04 to 0.10. These values showed that A. indica from TN Baluran was not genetically diverse population

Analisis Keragaman Genetik Musa Balbisiana Colla Berdasarkan Marka Rapd Dan Issr [Genetic Variation Analyses of Musa Balbisiana Colla Based on Rapd and Issr Markers]

Wild Musa balbisiana Colla is one of the progenitors of cultivated bananas and plantains. It is originated in Asia, and distributed from India to Papua New Guinea. This study was conducted to assess the molecular diversity of 25 accessions of M. balbisiana based on Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) analyses. RAPD and ISSR fingerprints of these banana varieties were detected by amplifications of nine primers of RAPDs and six primers of ISSRs. RAPD primers produced 84 amplified fragments varying from 150 bp to 2300 bp in size. 21.43 % of the amplification bands were polymorphic. ISSR primers produced 61 amplified fragments varying from 250 bp to 2200 bp in size. 29.30 % of the amplification bands were polymorphic. Based on these results, the 25 accessions of Indonesian M. balbisiana showed a low genetic variation, with coefficient of similarity ranged from 0.81 to 0.99

Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers

Poerba YS, Ahmad F (2010) Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers. Biodiversitas 11: 118-123. This study was done to assess the molecular diversity of 36 accessions (18 cultivars) of the plantain and cooking bananas (Musa acuminata x M. balbisiana, AAB, ABB subgroups) based on Random amplified polymorphic DNA (RAPD) and and Inter Simple Sequence Repeats (ISSR) markers and to determine genetic relationships in the bananas. RAPD and ISSR fingerprinting of these banana varieties was carried out by five primers of RAPDs and two primers of ISSRs. RAPD primers produced 63 amplified fragments varying from 250 to 2500 bp in size. 96.82% of the amplification bands were polymorphic. ISSR primers produced 26 amplified fragments varying from 350 bp to 2000 bp in size. The results showed that 92.86% of the amplification bands were polymorphic. The range of genetic distance of 18 cultivars was from 0.06-0.67

Keragaman Genetik Kultivar Pisang Diploid (AA) Koleksi Cibinong Science Center Berdasarkan Marka RAPD dan ISSR

The banana (Musa acuminata Colla) is considered as an important crop plant due to its high economic value which also has good dietary source. Here, the genetic variation of 20 diploid (AA) banana cultivars from Cibinong Science Center collection were analyzed. Random amplified polymorphic DNAs (RAPDs) and Inter Simple Sequence Repeats fingerprinting of these banana cultivars were carried out by four primers of RPDSs and two primers of inter simple sequence repeats (ISSRs) led to DNA amplification. The amplification products of RPADs and ISSRs were polymorphic, 97.83% and 95%, respectively. Size of the bands was varied from 350bp to 2.0 kbp. The range of genetic distance was from 0.06 to 0.07. The molecular data showed that these banana varieties were diverse collection

Identifikasi Tingkat Ploidi Tanaman Pisang Menggunakan Flow Cytometer di Jawa Tengah

In Indonesia, banana plants are known to have high genetic diversity, both cultivated and wild bananas. Banana plants are naturally known to have several levels of ploidy, namely diploid, triploid, and tetraploid. This study aims to identify the level of ploidy in banana plants explored in Central Java. The research method used flow cytometer, which analyzes the size of the plant genome, thus providing an overview of the level of ploidy and genetic diversity in banana plant populations. The research samples were banana plant leaves from various exploration locations in Central Java. The identification results show that of the 30 banana plant leaf samples that were successfully analyzed, it is known that the largest number of samples from banana plants that have triploid ploidy level (2n = 3x), namely 15 numbers, diploid ploidy level (2n = 2x) as many as 14 numbers, and found 1 cultivated banana with tetraploid ploidy level (2n = 4x). The results of ploidy level identification can provide guidance for researchers and plant breeders in selecting the right genotype for the development of superior varieties.Di Indonesia tanaman pisang diketahui mempunyai keragaman genetik yang cukup tinggi, baik pisang budi daya maupun pisang liar. Secara alami tanaman pisang diketahui mempunyai beberapa tingkat ploidi yaitu diploid, triploid, dan tetraploid. Penelitian ini bertujuan untuk mengidentifikasi tingkat ploidi pada tanaman pisang hasil eksplorasi di Jawa Tengah. Metode penelitian menggunakan flow cytometer yaitu menganalisis ukuran genom tanaman, sehingga memberikan gambaran mengenai tingkat ploidi dan keragaman genetik pada populasi tanaman pisang. Sampel penelitian berupa daun tanaman pisang yang berasal dari berbagai lokasi eksplorasi di daerah Jawa Tengah. Hasil identifikasi menunjukkan bahwa dari 30 sampel daun tanaman pisang yang berhasil dianalisis, diketahui bahwa jumlah sampel terbanyak dari tanaman pisang yang memiliki tingkat ploidi triploid (2n=3x) yaitu 15 nomor, tingkat ploidi diploid (2n=2x) sebanyak 14 nomor, dan ditemukan 1 pisang budi daya dengan tingkat ploidi tetraploid (2n=4x). Hasil identifikasi tingkat ploidi dapat memberikan panduan bagi peneliti dan pemulia tanaman dalam pemilihan genotipe yang tepat untuk pengembangan varietas unggul

Genetic Variation Of Wild Musa Acuminata Colla From Indonesia Based On Rapd And Issr Markers

Musa acuminata Colla is one of the progenitors of cultivated bananas today, and Indonesia is the center of origin and diversity of M. acuminata. Genetic variation of wild M. acuminata from Indonesia has not been studied extensively. This study was conducted to assess genetic variation of wild M. acuminata from Indonesia based on 10 RAPD (Random Amplified Polymorphic DNA) and 10 ISSR (Inter Simple Sequence Repeats) markers. The genetic properties of 17 populations of wild M. acuminata were analyzed by Popgene 1.32 based on Nei\u27s unbiased measures of genetic identity and genetic distance. A total of 443 DNA bands were produced, 425 (95.94%) of which were polymorphic. Cluster analysis of combined data of RAPD and ISSR produced a dendrogram which separated the population of M. acuminata (A genome) from M. balbisiana (B genome), but not from M. schizocarpa (S genome). Genetic distance (Nei, 1978) of the 17 populations of wild M. acuminata ranged from 0.3676 to 0.1634. The highest genetic distance was observed between M. acuminata var rutilifes (from East Java) and M. acuminata var sumatrana (from West Sumatra). The percentages of polymorphic loci among the 17 populations of M. acuminata ranged from 9.93% to 39.73%. Nei\u27s (1973) gene diversity (h) ranged from 0.041 to 0.1418. M. acuminata var malaccensis population was the most diverse population among the 17 M. acuminata populations studied. The high level of genetic diversity of the wild M. acuminata from Indonesia emphasizes the need for conservation and preservation of the natural population and its use in banana breeding program