Prevalence and diagnostic test comparison of Brucellosis in cattle in Pabna and Mymensingh districts of Bangladesh

Present study was undertaken to determine the seroprevalence of brucellosis in cattle of Pabna and Mymensingh districts in Bangladesh. A total of 260 cattle sera samples were collected from Pahna and Mymensingh districts. The epidemiological data were collected by structured questionnaire. RBT and SAT were used as screening tests and further confirmed by 1-ELISA. The seroprevalence of Bruce/Ia ill cattle was estimated to be 4.23%, 3.07% and 2.31% by RBT, SAT and 1-ELISA, respectively. The comparison of the serological tests result revealed the highest prevalence in RBT than SAT and 1-EL ISA. The prevalence of Brucella was 2.5% in Pabna and 2.14% in Mymensingh. It was observed that, a higher prevalence of Bruce/la was found in female (2.67%) than in male (1.82%), natural breeding (2.67%) than artificial breeding (1.81%), in aged animals (3.33%) than young (1.25 degree percent). But these differences were not statistically significant. There exists significant difference between prevalence of Bruce/II in cattle with history of abortion than without history of abortion (P value=0.013)

Evaluation of avian influenza and Newcastle disease virus detection kit using field samples from domestic and Semi-domestic birds

The study was undertaken to evaluate sensitivity and specificity of rapid Avian Influenza (AI) and Newcastle Disease virus (NDV) combo antigen kits from field samples of domestic (broiler and layer chicken, native chicken) and semi-domestic (duck, goose, pigeon and quail) birds of Bangladesh. Samples were collected from naturally infected AI suspected domestic and semi-domestic birds of five different outbreak areas in Bangladesh. From each area two birds were selected for sampling, and from each bird three types of samples (tracheal, cloacal and oro-nasal swabs) were collected. A total of 210 field samples from a total of 70 birds were collected and tested using AI and NDV combo antigen rapid diagnostic kits in the study. All three different samples from a bird showed similar pattern of reaction. Out of 210 samples, 15 samples (5 birds), 63 samples (21 birds) and 27 samples (9 birds) were positive for AIV, NDV and both for AIV and NDV, respectively; whereas the remaining birds were negative for either AIV or NDV in this screening test. Among the five AIV positive, a layer chicken from wet market in Mymensingh, Netrokona, Gibandha and Kurigram and a native chicken from wet market in Kurigram area was positive to AIV. The semi-domestic birds are either positive to NDV or free from both AIV and NDV. This study revealed that the AIV and NDV rapid diagnostic kits could be effectively use to diagnose the respective virus in trachea, oro-nasal and cloacal samples simultaneously. AIV-NDV combo Ag test result clearly indicates that the test kit designed for AIV and NDV could diagnose the disease rapidly with less effort and higher scientific know how which could be used for the detection of AIV and NDV using field samples in large scale

Tube agglutination test is superior than other serological tests for diagnosis of brucellosis in small ruminants.

Brucella spp. are small, non-motile Gram-negative coccobacilli known to cause disease in a number of vertebrate species including humans and brucellosis is one of the world's major zoonoses, alongside bovine tuberculosis and rabies. There are about 33.55 million goats and 1.16 million sheep in Bangladesh. The sheep and goats can significantly play an important role in the economic well being of the resource-poor farmer in Bangladesh. Sexually matured 362 female small ruminants(300 goats and 62 sheep) were examined. Approximately 3-5 ml of blood was collected from the jugular vein of each animal and sera samples were prepared. Samples were then tested for brucellosis by using Rose Bengal test(RBT), plate agglutination test(PAT) and tube agglutination test(TAT). Among 362 small ruminants, irrespective of species(sheep or goat), diagnosed highest in TAT, 2.21%(n=8) and lowest both by RBT & PAT, 1.93%(n=7) and it is concluded that TAT is superior than RBT and PAT