3 research outputs found
Guillain–Barré syndrome in Bangladesh: The role TLR4 Asp299Gly and Thr399Ile polymorphisms
Toll-like receptor-4 299Gly allele is associated with Guillain-Barre syndrome in Bangladesh
Objective: TLR4 plays an important role in the pathogenesis of Guillain-Barre
syndrome (GBS). The relationships between TLR4 polymorphisms and susceptibility to GBS are poorly understood. We investigated the frequency and
assessed the association of two single nucleotide polymorphisms (SNPs) in the
extracellular domain of TLR4 (Asp299Gly and Thr399Ile) with disease susceptibility and the clinical features of GBS in a Bangladeshi cohort. Methods: A total
of 290 subjects were included in this study: 141 patients with GBS and 149
unrelated healthy controls. The TLR4 polymorphisms Asp299Gly and Thr399Ile
were genotyped using polymerase chain reaction-restriction fragment length
polymorphism (PCR-RFLP) assay. Results: The minor 299Gly allele was significantly associated with GBS susceptibility (P = 0.0137, OR = 1.97, 95%
CI = 1.17–3.31), and was present at a significantly higher frequency in patients
with the acute motor axonal neuropathy (AMAN) subtype of GBS (P = 0.0120,
OR = 2.37, 95% CI = 1.26–4.47) than acute inflammatory demyelinating
polyneuropathy (AIDP) subtype (P = 0.961, OR = 1.15, 95% CI = 0.38–3.48);
when compared to healthy controls. The genotype frequency of the Asp299Gly
polymorphism was not significantly different between patients with GBS and
healthy controls. The Asp299-Thr399 haplotype was associated with a significantly lower risk of developing GBS (P = 0.0451, OR = 0.63, 95% CI = 0.40–
0.99). No association was observed between the Thr399Ile polymorphism and
GBS disease susceptibility. Interpretation: The TLR4 minor 299Gly allele was
associated with increased susceptibility to GBS and the axonal GBS subtype in
the Bangladeshi population. However, no associations were observed between
the genotypes of the Asp299Gly and Thr399Ile SNPs and antecedent C. jejuni
infection or disease severity in Bangladeshi patients with GBS