Response of New Yellow Lupin Varieties to Inoculation with <i>Bradyrhizobium</i> sp. <i>Lupinus</i> under Central European Conditions

The aim of a two-factorial field experiment was to determine how the inoculation of seeds/soil with preparations of Bradyrhizobium sp. Lupinus (Nitragina—seed inoculation, Nitroflora I—seed inoculation, Nitroflora II—soil inoculation, HiStick® Lupin—seed inoculation) affected plant development, seed chemical composition and yield of two yellow lupin varieties (Bursztyn, Puma). This experiment was carried out with four replications in 2018 and 2019 in Poland. Precipitation during both vegetation periods was similar to or lower than the long-term mean. Average seed yield of Puma was significantly greater than Bursztyn (by 0.22 t ha−1). According to the correlation coefficients, seed yield was mainly related to plant height, dry mass of nodules per plant and mass of 1000 seeds. Our results suggest that legumes, such as lupin, should always be inoculated with Bradyrhizobium, especially if they are cultivated for the first time in a field. For optimal results, the highest-quality preparations should be used. In our study, the best results were observed after HiStick® Lupin inoculation, which resulted in the highest protein content, seed yield and protein yield across all treatments

QUALITY AND HYGIENIC CONDITIONS OF WHITE LUPIN SILAGE, AFFECTED BY FORAGE STAGE OF GROWTH AND USE OF SILAGE ADDITIVES

ABSTRACT A two-factor field experiment with white lupin cv. Butan was carried out. The first factor was the green forage harvest date (the flat pod stage -Cut 1 and the stage of green ripe seeds -Cut 2), while the second one -application of silage additives: biological (strains of lactic acid bacteria) and chemical (a mixture of organic acids), and the control treatment (without additives). In Cut 2 higher fresh matter (FM) and dry matter (DM) yields were obtained. Silage inoculated with the biological additive contained a significantly greater count of lactic acid bacteria. Both additives reduced counts the Clostridium bacteria, yeasts and mould fungi. The silage with the chemical additive had a three-fold higher content of water-soluble carbohydrates (WSC), while the biological additive increased lactic acid (LA) levels. White lupin can be used as a silage raw material, but plants before ensiling should be partially wilted and silage additives should be applied

The effect of soil type and soil additives on the selected growth parameters and yield of flowerheads of Calendula officinalis L.

Introduction: Soil additives, which usually contain nutrients and microorganism, can improve soil conditions for plants. There are still few papers dedicated to the application of soil additives in herb plants, especially in calendula

QUALITY AND HYGIENIC CONDITIONS OF WHITE LUPIN SILAGE, AFFECTED BY FORAGE STAGE OF GROWTH AND USE OF SILAGE ADDITIVES

ABSTRACT A two-factor field experiment with white lupin cv. Butan was carried out. The first factor was the green forage harvest date (the flat pod stage -Cut 1 and the stage of green ripe seeds -Cut 2), while the second one -application of silage additives: biological (strains of lactic acid bacteria) and chemical (a mixture of organic acids), and the control treatment (without additives). In Cut 2 higher fresh matter (FM) and dry matter (DM) yields were obtained. Silage inoculated with the biological additive contained a significantly greater count of lactic acid bacteria. Both additives reduced counts the Clostridium bacteria, yeasts and mould fungi. The silage with the chemical additive had a three-fold higher content of water-soluble carbohydrates (WSC), while the biological additive increased lactic acid (LA) levels. White lupin can be used as a silage raw material, but plants before ensiling should be partially wilted and silage additives should be applied

QUALITY AND HYGIENIC CONDITIONS OF WHITE LUPIN SILAGE, AFFECTED BY FORAGE STAGE OF GROWTH AND USE OF SILAGE ADDITIVES

ABSTRACT A two-factor field experiment with white lupin cv. Butan was carried out. The first factor was the green forage harvest date (the flat pod stage -Cut 1 and the stage of green ripe seeds -Cut 2), while the second one -application of silage additives: biological (strains of lactic acid bacteria) and chemical (a mixture of organic acids), and the control treatment (without additives). In Cut 2 higher fresh matter (FM) and dry matter (DM) yields were obtained. Silage inoculated with the biological additive contained a significantly greater count of lactic acid bacteria. Both additives reduced counts the Clostridium bacteria, yeasts and mould fungi. The silage with the chemical additive had a three-fold higher content of water-soluble carbohydrates (WSC), while the biological additive increased lactic acid (LA) levels. White lupin can be used as a silage raw material, but plants before ensiling should be partially wilted and silage additives should be applied

Grzyby zasiedlające nasiona trzech gatunków łubinów uprawianych w różnych rejonach Polski

The health status of seeds of three lupine species (white lupine, narrow-leaved lupine, and yellow lupine) from different regions of Poland was investigated. Seeds were analyzed by microscopic method and PCR. The examined lupine seeds were colonized by saprotrophic fungi of the genera Alternaria, Cladosporium, and Penicillium, and pathogenic fungi of the genera Fusarium, Botrytis, Mycosphaerella, and Colletotrichum. The relative frequency (RF) of fungi detected on lupine seeds from the regions of Kujawy, Wielkopolska, Lower Silesia, and Warmia and Mazury was determined. The highest RF values of pathogenic fungi were noted in Lower Silesia in 2012 and 2013, and in Warmia and Mazury in 2011. The RF values of pathogenic and saprotrophic fungi on lupine seeds harvested in different regions of Poland were affected by weather conditions. PCR analyses revealed the presence of Tri genes in the seeds of narrow-leaved lupine. The analyzed seeds were relatively free of pathogenic fungi and could be used for sowing and feed production.Badania dotyczyły oceny zdrowotności nasion trzech gatunków łubinów (biały, wąskolistny, żółty) pochodzących z różnych rejonów Polski. Analizy wykonano metodą mikroskopową oraz PCR. Wykazano, że nasiona badanych łubinów były zasiedlone przez grzyby saprotroficzne: Alternaria spp. Cladosporium spp. Penicillium spp. oraz patogeniczne z rodzajów: Fusarium, Botrytis, Mycosphaerella i Colletotrichum. Obliczono wskaźnik częstotliwości występowania grzybów (RF) w nasionach łubinów pochodzących z Kujaw, Wielkopolski, Dolnego Śląska oraz Warmii i Mazur. Wykazano, że najwyższe wartości wskaźnika częstotliwości występowania gatunków patogenicznych dotyczyły nasion łubinów pochodzących z rejonu Dolnego Śląska w roku 2012 i 2013 oraz z Warmii i Mazur w roku 2011. Stwierdzono, że na częstotliwość występowania grzybów patogenicznych i spartotroficznych na nasionach pozyskanych z różnych regionów Polski wpływ miały warunki pogodowe. Wykazano ponadto obecność genów Tri w nasionach łubinu wąskolistnego metodą PCR. Stwierdzono, że nasiona badanych łubinów były w niewielkim stopniu zasiedlone przez grzyby patogeniczne i mogą stanowić dobry surowiec do produkcji pasz oraz jako materiał nasienny

Optimizing the Amount of Nitrogen and Seed Inoculation to Improve the Quality and Yield of Soybean Grown in the Southeastern Baltic Region

The cultivation of soybeans, especially where this species has not been grown in large areas, requires the determination of the optimal mineral nitrogen fertilization and seed inoculation with Bradyrhizobium japonicum. The purpose of the study was to determine the optimal dose of mineral N fertilization and seed inoculation treatments with B. japonicum under field conditions in the southeastern Baltic region. The objective of this study was to achieve nitrogen supply and/or inoculation with B. japonicum: check-0 kg N ha−1, 30 kg N ha−1, 60 kg N ha−1, HiStick® Soy + 0 kg N ha−1, Nitroflora + 0 kg N ha−1, HiStick® Soy + 30 kg N ha−1, HiStick® Soy + 60 kg N ha−1, Nitroflora + 30 kg N ha−1, Nitroflora + 60 kg N ha−1. Higher yields of seeds, protein and fat were found after application HiStick® Soy compared to Nitorflora. The inoculation with B. japonicum together with nitrogen fertilization improved crude protein content in seeds, biometrical features, yield components and especially the seed yield of ‘Aldana’ soybean. The highest seed yield was found after the application of HiStick® Soy and nitrogen fertilization in doses 30 kg N ha−1 or 60 kg N ha−1. Compared to the control, combined B. japonicum inoculation and nitrogen fertilization in soybean cultivation proved to be a significant factor in improving the productivity of this species in southeastern Baltic conditions